10KY
X-ray structure of the Bacteroides fragilis Nramp/MntH divalent transition metal transporter WT in an inward-open, state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 69.119, 102.272, 119.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.140 - 2.480 |
| R-factor | 0.2615 |
| Rwork | 0.259 |
| R-free | 0.29850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.389 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.140 | 2.540 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.341 | |
| Rmeas | 0.398 | |
| Rpim | 0.205 | |
| Number of reflections | 30582 | 30582 |
| <I/σ(I)> | 4.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 298 | 0.2 M ammonium sulfate, 0.02 M sodium chloride, 0.02 M sodium acetate pH 4.0, 33% PEG 200 |
