10KV

Structure of hNSP4 bound to MCP4 at 2.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2023-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	71.150, 71.150, 149.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.580 - 2.750
R-factor	0.2511
Rwork	0.250
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	0.813
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.580	2.780
High resolution limit [Å]	2.749	2.749
Rmerge	0.083	1.559
Number of reflections	7962	24
<I/σ(I)>	13.4
Completeness [%]	75.1
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M MES pH 6, 0.2M NaCl, 15% PEG MME 2000