10KU

Structure of hNSP4-S218A bound to MCP3 at 2.09 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	110.041, 43.669, 124.030
Unit cell angles	90.00, 92.61, 90.00

Refinement procedure

Resolution	123.900 - 2.090
R-factor	0.2299
Rwork	0.228
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.798
Data reduction software	autoPROC
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1-4487_final: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.900	2.165
High resolution limit [Å]	2.090	2.090
Number of reflections	54559	2728
<I/σ(I)>	5.8	1.8
Completeness [%]	77.2
Redundancy	4.4
CC(1/2)	0.985	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	25% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.2 M LiSO4