10JY

Crystal structure of heme binding PAS domain from one component transcription factor, FG214 reduced with dithionite

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	63.030, 65.210, 105.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.320 - 1.650
R-factor	0.1889
Rwork	0.188
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.117
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.523	1.677
High resolution limit [Å]	1.648	1.648
Number of reflections	393593	20404
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.999	0.382

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Sodium formate, 0.2M Ammonium acetate, 0.2M Sodium citrate tribasic dihydrate, 0.2M Potassium sodium tartrate tetrahydrate, 0.2M Sodium oxamate, 0.2M 1,6-Hexanediol, 0.2M 1-Butanol 0.2M 1,2-Propanediol, 0.2M 2-Propanol, 0.2M 1,4-Butanediol, 0.2M 1,3-Propanediol, 0.4M Imidazole, 0.6M MES monohydrate, 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350