10JX

Crystal structure of heme binding PAS domain from one component transcription factor, FG214

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 21 21 21
Unit cell lengths	63.346, 64.932, 105.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.450 - 1.470
R-factor	0.1654
Rwork	0.164
R-free	0.19010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.451	1.493
High resolution limit [Å]	1.467	1.467
Number of reflections	75338	3680
<I/σ(I)>	9.8
Completeness [%]	99.9
Redundancy	7.7
CC(1/2)	0.992	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Sodium formate, 0.2M Ammonium acetate, 0.2M Sodium citrate tribasic dihydrate, 0.2M Potassium sodium tartrate tetrahydrate, 0.2M Sodium oxamate, 0.2M 1,6-Hexanediol, 0.2M 1-Butanol 0.2M 1,2-Propanediol, 0.2M 2-Propanol, 0.2M 1,4-Butanediol, 0.2M 1,3-Propanediol, 0.4M Imidazole, 0.6M MES monohydrate, 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350