10HS
[17,17,7-7N_234] Isosceles tensegrity triangle with 17, 17 and 7 base pairs between junctions and two, three, and four turn edges
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.007430 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.873, 102.070, 103.598 |
| Unit cell angles | 100.99, 96.52, 99.04 |
Refinement procedure
| Resolution | 42.070 - 6.830 |
| R-factor | 0.1228 |
| Rwork | 0.121 |
| R-free | 0.14420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.551 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.538 | 7.683 |
| High resolution limit [Å] | 6.797 | 6.797 |
| Number of reflections | 2571 | 199 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 80.8 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.234 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
