10HO

Crystal structure of alkaline nuclease from Herpes simplex virus-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 21 21 21
Unit cell lengths	58.997, 80.233, 127.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.642 - 2.460
Rwork	0.181
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.247
Data reduction software	FAST_DP
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.642	2.560
High resolution limit [Å]	2.460	2.460
Rmerge	0.037	0.316
Rmeas	0.052
Rpim	0.037	0.446
Number of reflections	22700	2508
<I/σ(I)>	13.8	2.4
Completeness [%]	99.9	99.2
Redundancy	1.9	1.9
CC(1/2)	0.999	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277.15	Ammonium sulfate, Sodium acetate, PEG 4000, Glycerol and DMSO