10HF

[17,17,7-7N_3T] Isosceles tensegrity triangle with 17, 17 and 7 base pairs between junctions, three turn edges

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000010
Spacegroup name	P 1
Unit cell lengths	91.665, 99.533, 100.317
Unit cell angles	83.09, 62.81, 66.03

Refinement procedure

Resolution	44.470 - 7.240
R-factor	0.1427
Rwork	0.142
R-free	0.16190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.797
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.944	88.944	8.357
High resolution limit [Å]	7.236	19.506	7.236
Number of reflections	2402	199	201
<I/σ(I)>	4.9
Completeness [%]	83.5
Redundancy	3.7
CC(1/2)	0.983	0.978	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM MOPS, 1.25 M magnesium sulfate