10HD

[17,17,7-17N_332] Isosceles tensegrity triangle with 17, 17 and 7 bp between junctions and 3, 3, and 2 turns of DNA per edge

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000010
Spacegroup name	P 1
Unit cell lengths	68.035, 86.395, 101.431
Unit cell angles	106.32, 101.56, 111.48

Refinement procedure

Resolution	45.780 - 5.260
R-factor	0.1511
Rwork	0.149
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.889
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.073	5.927
High resolution limit [Å]	5.260	5.260
Number of reflections	4151	208
<I/σ(I)>	9.6
Completeness [%]	84.9
Redundancy	3.6
CC(1/2)	0.999	0.304

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM MOPS, 1.25 M magnesium sulfate