10HA
Crystal Structure of Honeybee (Apis mellifera) omega class glutathione S-transferase AmGST-O1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.143, 78.385, 107.966 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 61.090 - 2.050 |
| R-factor | 0.2221 |
| Rwork | 0.221 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.090 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.089 | 0.540 |
| Rmeas | 0.107 | 0.644 |
| Rpim | 0.059 | 0.347 |
| Number of reflections | 63482 | 4529 |
| <I/σ(I)> | 6.09 | 1.79 |
| Completeness [%] | 98.4 | 98.8 |
| Redundancy | 3.2 | 3.3 |
| CC(1/2) | 0.966 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium acetate and 20% PEG 3,350 |
