10FY
[112SE] Two turn tensegrity triangle with 1,1 and 2 bp sticky ends
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.923, 68.115, 69.672 |
| Unit cell angles | 97.10, 101.20, 98.55 |
Refinement procedure
| Resolution | 43.040 - 3.900 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.985 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.137 | 4.446 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 6774 | 339 |
| <I/σ(I)> | 8.1 | 1.1 |
| Completeness [%] | 84.8 | |
| Redundancy | 3.6 | |
| CC(1/2) | 1.000 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
