10FV

Crystal Structure of Human Fgr SH3-SH2-High Affinity Linker Mutant 2 (HAL2) Domains.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	43.393, 35.127, 60.846
Unit cell angles	90.00, 100.64, 90.00

Refinement procedure

Resolution	42.650 - 1.800
Rwork	0.194
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.629
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.650	42.650	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.029	0.011	0.621
Rmeas	0.041	0.016	0.879
Rpim	0.029	0.011	0.621
Number of reflections	16998	155	999
<I/σ(I)>	9.7	28.7	1.1
Completeness [%]	100.0	99.2	100
Redundancy	1.9	1.7	2
CC(1/2)	0.999	0.999	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298.15	0.1 M trisodium citrate pH 5.5, 2.5 M ammonium sulfate