10FS

Crystal Structure of Human Fgr SH3-SH2-High Affinity Linker Mutant 1 (HAL1) Domains.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	44.443, 34.794, 61.677
Unit cell angles	90.00, 101.05, 90.00

Refinement procedure

Resolution	39.120 - 1.300
Rwork	0.182
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.900
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.120	39.120	1.320
High resolution limit [Å]	1.300	7.120	1.300
Rmerge	0.068	0.042	3.591
Rmeas	0.070	0.044	3.732
Rpim	0.019	0.012	1.005
Number of reflections	45366	309	2217
<I/σ(I)>	14.9	62.3	0.9
Completeness [%]	99.0	99.4	98.8
Redundancy	13.4	12.8	13.2
CC(1/2)	1.000	0.998	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298.15	0.2 M sodium chloride, 0.1 M MES monohydrate, pH 6.0, 20% (w/v) polyethylene glycol 6,000