10DT

Crystal Structure of Human Wild-Type Fgr SH3-SH2-Linker Domains.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	44.185, 34.867, 60.109
Unit cell angles	90.00, 100.75, 90.00

Refinement procedure

Resolution	38.550 - 1.800
Rwork	0.166
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.270
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.550	38.550	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.231	0.071	1.430
Rmeas	0.259	0.081	1.610
Rpim	0.115	0.037	0.722
Number of reflections	16655	152	994
<I/σ(I)>	5.5	9.9	1.7
Completeness [%]	98.6	98.8	97.4
Redundancy	4.5	4.2	4.3
CC(1/2)	0.981	0.991	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298.15	0.1 M Bis-Tris pH 5.5, 0.2 M NaCl, 25% (w/v) PEG 3350