10CW
Crystal structure of Thermoproteus neutrophilus Rpp30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-06 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9211 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.758, 66.368, 66.056 |
| Unit cell angles | 90.00, 113.81, 90.00 |
Refinement procedure
| Resolution | 32.970 - 1.430 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.23240 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20190315) |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.600 |
| High resolution limit [Å] | 1.400 | 1.430 |
| Rmerge | 0.217 | 0.218 |
| Rmeas | 0.227 | 0.234 |
| Number of reflections | 60247 | 3012 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 74.4 | 16.3 |
| Redundancy | 10.2 | 7.2 |
| CC(1/2) | 0.998 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 303 | Crystals were grown by vapor diffusion equilibrated with 100 mM CHES, pH 9.5, 35% (v/v) PEG 400 at 303 K. Crystals were sufficiently cryo-protected by the crystallization solution containing 35% (v/v) PEG 400 without further supplementation prior to flash freezing |
