10CH

[011SE] Two turn tensegrity triangle with 0,1 and 1 bp sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000000
Spacegroup name	P 1
Unit cell lengths	67.618, 68.133, 69.809
Unit cell angles	96.73, 98.46, 99.85

Refinement procedure

Resolution	40.930 - 4.450
R-factor	0.2169
Rwork	0.216
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.995
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.927	4.936
High resolution limit [Å]	4.446	4.446
Number of reflections	4589	230
<I/σ(I)>	5.8
Completeness [%]	82.3
Redundancy	3.6	3.8
CC(1/2)	0.999	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM MOPS, 1.25 M magnesium sulfate