10AJ
Crystal Structure of Human WRN helicase with compound 1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.130, 58.180, 131.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.940 - 2.420 |
| Rwork | 0.211 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.940 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.078 | 1.550 |
| Rpim | 0.027 | |
| Number of reflections | 17771 | 870 |
| <I/σ(I)> | 15.3 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 7.7 |
| CC(1/2) | 0.999 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290 | protocol was established using PROTEROS Standard Protocols |
