10TC

Structure of WDR5 bound to RbBP5 (D376N)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2023-07-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	78.480, 105.711, 80.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.430 - 2.200
R-factor	0.1877
Rwork	0.185
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.200	2.200
Rmeas	0.080	0.380
Number of reflections	65288	3248
<I/σ(I)>	3.1
Completeness [%]	97.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.15 M Ammonium citrate, 18% PEG3350