10RC

Human SARM1 TIR domain bound to compound 22

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9530
Spacegroup name	P 2 21 21
Unit cell lengths	33.332, 85.794, 116.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.090 - 1.900
Rwork	0.214
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.090	1.960
High resolution limit [Å]	1.900	1.900
Number of reflections	27262	2644
<I/σ(I)>	8.2	1.1
Completeness [%]	99.7
Redundancy	7.3
CC(1/2)	0.999	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M Bis-Tris-Propane, pH 6.5, 0.2 M K-thiocyanate, 7.5% PEG 3350