10BT
X-ray Crystal Structure of A High-Affinity Monoclonal Antibody Sequesters Xylazine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.919887 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.178, 121.034, 95.621 |
| Unit cell angles | 90.00, 112.45, 90.00 |
Refinement procedure
| Resolution | 41.990 - 1.990 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.21.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.187 | 2.341 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.157 | 0.849 |
| Rmeas | 0.179 | 0.962 |
| Rpim | 0.083 | 0.449 |
| Total number of observations | 310426 | 15433 |
| Number of reflections | 68933 | 3447 |
| <I/σ(I)> | 4.3 | 1.6 |
| Completeness [%] | 53.7 | |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | J002007, F05_10 100 mM Tris base, pH 8, 8 (%w/v) PEG 20000, 100 mM Sodium chloride |
