100D

CRYSTAL STRUCTURE OF THE HIGHLY DISTORTED CHIMERIC DECAMER R(C)D(CGGCGCCG)R(G)-SPERMINE COMPLEX-SPERMINE BINDING TO PHOSPHATE ONLY AND MINOR GROOVE TERTIARY BASE-PAIRING

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.9 Å)

Cell axes23.98040.77044.840
Cell angles90.0090.0090.00
SpacegroupP 21 21 21
Resolution limits8.00 - 1.90
the highest resolution shell value -
R-factor0.145
R-work0.14500 (0.00000*)
RMSD bond length0.015
RMSD bond angle3.800

Data Collection Statistics

Resolution limits - 1.90
the highest resolution shell value -
Number of reflections2365
Rmerge_l_obs0.058*

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP7unknown*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
111WATER
211MPD
311NA CACODYLATE
411SPERMINE_HCL
512WATER
612MPD
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropdecamer duplex1 (mM)
21dropsodium cacodylate40 (mM)
31dropspermine tetrachloride2.0 (mM)
41reservoirMPD45 (%)
Annotated Information is extracted from Literature Info*