100D
CRYSTAL STRUCTURE OF THE HIGHLY DISTORTED CHIMERIC DECAMER R(C)D(CGGCGCCG)R(G)-SPERMINE COMPLEX-SPERMINE BINDING TO PHOSPHATE ONLY AND MINOR GROOVE TERTIARY BASE-PAIRING
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 263 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.980, 40.770, 44.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.145 |
| Rwork | 0.145 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.800 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.058 * |
| Number of reflections | 2365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | decamer duplex | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 2.0 (mM) | |
| 4 | 1 | reservoir | MPD | 45 (%) |
