PDB-5tbw: Crystal structure of chlorolissoclimide bound to the yeast 80S ri...

Basic information

• Entry: Database: PDB / ID: 5tbw
• Title: Crystal structure of chlorolissoclimide bound to the yeast 80S ribosome

Components

• (40S ribosomal protein ...) x 32
• (60S ribosomal protein ...) x 40
• (Suppressor protein ...) x 2
• (Ubiquitin-40S ribosomal protein ...) x 2
• 18S ribosomal RNA
• 25S ribosomal RNA
• 5.8S ribosomal RNA
• 5S ribosomal RNA
• 60S acidic ribosomal protein P0
• Guanine nucleotide-binding protein subunit beta-like protein
• Ubiquitin-60S ribosomal protein L40

• Keywords: RIBOSOME / Protein translation inhibitor / complex

Function / homology

Function:

triplex DNA binding / ribosome hibernation / translation elongation factor binding / regulation of translational initiation in response to stress / Platelet degranulation / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Protein methylation / RMTs methylate histone arginines / positive regulation of translational fidelity / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / Protein hydroxylation / GDP-dissociation inhibitor activity / : / pre-mRNA 5'-splice site binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / translational elongation / response to cycloheximide / telomeric DNA binding / mRNA destabilization / TOR signaling / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / protein-RNA complex assembly / ribosomal small subunit export from nucleus / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / positive regulation of protein kinase activity / rescue of stalled ribosome / translational termination / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation repressor activity / maturation of LSU-rRNA / ribosomal large subunit biogenesis / DNA-(apurinic or apyrimidinic site) endonuclease activity / cellular response to amino acid starvation / ribosome assembly / telomere maintenance / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / macroautophagy / modification-dependent protein catabolic process / ribosomal small subunit biogenesis / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / cytoplasmic stress granule / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / ribosome binding / ribosome biogenesis / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / negative regulation of apoptotic process / perinuclear region of cytoplasm / mitochondrion / DNA binding / RNA binding / zinc ion binding / nucleoplasm / metal ion binding

Domain/homology:

Stm1-like, N-terminal / Stm1 / Hyaluronan/mRNA-binding protein / Hyaluronan / mRNA binding family / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S12e / 50S ribosomal protein L10, insertion domain superfamily / : / Small (40S) ribosomal subunit Asc1/RACK1 / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal L29e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / : / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 40S Ribosomal protein S10 / 60S ribosomal protein L35 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein S7e / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site

Component:

Chlorolissoclimide / osmium (III) hexammine / : / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein eS17A / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS17B / Large ribosomal subunit protein eL8A / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS10 / Suppressor protein STM1 / Small ribosomal subunit protein uS11B / Small ribosomal subunit protein eS26B / Small ribosomal subunit protein uS14A / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å
• Authors: Konst, Z.A. / Szklarski, A.R. / Pellegrino, S. / Michalak, S.E. / Meyer, M. / Zanette, C. / Cencic, R. / Nam, S. / Horne, D.A. / Pelletier, J. / Mobley, D.L. / Yusupova, G. / Yusupov, M. / Vanderwal, C.D.

Funding support

United States, France, 4items

Organization	Grant number	Country
National Institutes of Health	GM-086483	United States
UC Cancer Research Coordinating Committee	UCCRCC- 55179	United States
European Research Council	294312	France
French National Research Agency	ANR-15-CE11-0021-01	France

• Citation: Journal: Nat Chem / Year: 2017; Title: Synthesis facilitates an understanding of the structural basis for translation inhibition by the lissoclimides.; Authors: Konst, Z.A. / Szklarski, A.R. / Pellegrino, S. / Michalak, S.E. / Meyer, M. / Zanette, C. / Cencic, R. / Nam, S. / Voora, V.K. / Horne, D.A. / Pelletier, J. / Mobley, D.L. / Yusupova, G. / Yusupov, M. / Vanderwal, C.D.

History

Deposition	Sep 13, 2016	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jul 26, 2017	Provider: repository / Type: Initial release
Revision 1.1	Nov 1, 2017	Group: Author supporting evidence / Database references / Derived calculations Category: citation / pdbx_audit_support / struct_conn / struct_conn_type Item: _citation.journal_abbrev / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _pdbx_audit_support.funding_organization
Revision 1.2	Aug 8, 2018	Group: Author supporting evidence / Data collection / Database references / Source and taxonomy Category: citation_author / entity_src_gen / pdbx_audit_support Item: _citation_author.identifier_ORCID / _entity_src_gen.gene_src_common_name / _entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id / _entity_src_gen.pdbx_gene_src_scientific_name / _pdbx_audit_support.funding_organization
Revision 1.3	Jan 17, 2024	Group: Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

Assembly

Deposited unit

1: 25S ribosomal RNA

3: 5S ribosomal RNA

4: 5.8S ribosomal RNA

j: 60S ribosomal protein L2-A

k: 60S ribosomal protein L3

l: 60S ribosomal protein L4-A

m: 60S ribosomal protein L5

n: 60S ribosomal protein L6-A

o: 60S ribosomal protein L7-A

p: 60S ribosomal protein L8-A

q: 60S ribosomal protein L9-A

r: 60S ribosomal protein L10

s: 60S ribosomal protein L11-B

t: 60S ribosomal protein L13-A

u: 60S ribosomal protein L14-A

v: 60S ribosomal protein L15-A

w: 60S ribosomal protein L16-A

x: 60S ribosomal protein L17-A

y: 60S ribosomal protein L18-A

z: 60S ribosomal protein L19-A

0: 60S ribosomal protein L20-A

2: 60S ribosomal protein L21-A

5: 60S ribosomal protein L22-A

6: 60S ribosomal protein L23-A

7: 60S ribosomal protein L24-A

8: 60S ribosomal protein L25

9: 60S ribosomal protein L26-A

AA: 60S ribosomal protein L27-A

AB: 60S ribosomal protein L28

AC: 60S ribosomal protein L29

AD: 60S ribosomal protein L30

AE: 60S ribosomal protein L31-A

AF: 60S ribosomal protein L32

AG: 60S ribosomal protein L33-A

AH: 60S ribosomal protein L34-A

AI: 60S ribosomal protein L35-A

AJ: 60S ribosomal protein L36-A

AK: 60S ribosomal protein L37-A

AL: 60S ribosomal protein L38

AM: 60S ribosomal protein L39

AN: Ubiquitin-60S ribosomal protein L40

AO: 60S ribosomal protein L41-A

AP: 60S ribosomal protein L42-A

AQ: 60S ribosomal protein L43-A

i: Suppressor protein STM1

AR: 25S ribosomal RNA

AS: 5S ribosomal RNA

AT: 5.8S ribosomal RNA

CD: 60S ribosomal protein L2-A

CE: 60S ribosomal protein L3

CF: 60S ribosomal protein L4-A

CG: 60S ribosomal protein L5

CH: 60S ribosomal protein L6-A

CI: 60S ribosomal protein L7-A

CJ: 60S ribosomal protein L8-A

CK: 60S ribosomal protein L9-A

CL: 60S ribosomal protein L10

CM: 60S ribosomal protein L11-B

CN: 60S ribosomal protein L13-A

CO: 60S ribosomal protein L14-A

CP: 60S ribosomal protein L15-A

CQ: 60S ribosomal protein L16-A

CR: 60S ribosomal protein L17-A

CS: 60S ribosomal protein L18-A

CT: 60S ribosomal protein L19-A

CU: 60S ribosomal protein L20-A

CV: 60S ribosomal protein L21-A

CW: 60S ribosomal protein L22-A

CX: 60S ribosomal protein L23-A

CY: 60S ribosomal protein L24-A

CZ: 60S ribosomal protein L25

DA: 60S ribosomal protein L26-A

DB: 60S ribosomal protein L27-A

DC: 60S ribosomal protein L28

DD: 60S ribosomal protein L29

DE: 60S ribosomal protein L30

DF: 60S ribosomal protein L31-A

DG: 60S ribosomal protein L32

DH: 60S ribosomal protein L33-A

DI: 60S ribosomal protein L34-A

DJ: 60S ribosomal protein L35-A

DK: 60S ribosomal protein L36-A

DL: 60S ribosomal protein L37-A

DM: 60S ribosomal protein L38

DN: 60S ribosomal protein L39

DO: Ubiquitin-60S ribosomal protein L40

DP: 60S ribosomal protein L41-A

DQ: 60S ribosomal protein L42-A

DR: 60S ribosomal protein L43-A

p0: 60S acidic ribosomal protein P0

sM: Suppressor protein STM1

A: 18S ribosomal RNA

B: 40S ribosomal protein S0-A

C: 40S ribosomal protein S1-A

D: 40S ribosomal protein S2

E: 40S ribosomal protein S3

F: 40S ribosomal protein S4-A

G: 40S ribosomal protein S5

H: 40S ribosomal protein S6-A

I: 40S ribosomal protein S7-A

J: 40S ribosomal protein S8-A

K: 40S ribosomal protein S9-A

L: 40S ribosomal protein S10-A

M: 40S ribosomal protein S11-A

N: 40S ribosomal protein S12

O: 40S ribosomal protein S13

P: 40S ribosomal protein S14-B

Q: 40S ribosomal protein S15

R: 40S ribosomal protein S16-A

S: 40S ribosomal protein S17-A

T: 40S ribosomal protein S18-A

U: 40S ribosomal protein S19-A

V: 40S ribosomal protein S20

W: 40S ribosomal protein S21-A

X: 40S ribosomal protein S22-A

Y: 40S ribosomal protein S23-A

Z: 40S ribosomal protein S24-A

a: 40S ribosomal protein S25-A

b: 40S ribosomal protein S26-B

c: 40S ribosomal protein S27-A

d: 40S ribosomal protein S28-A

e: 40S ribosomal protein S29-A

f: 40S ribosomal protein S30-A

g: Ubiquitin-40S ribosomal protein S31

h: Guanine nucleotide-binding protein subunit beta-like protein

sR: 18S ribosomal RNA

Rb: Guanine nucleotide-binding protein subunit beta-like protein

s0: 40S ribosomal protein S0-A

s1: 40S ribosomal protein S1-A

s2: 40S ribosomal protein S2

s3: 40S ribosomal protein S3

s4: 40S ribosomal protein S4-A

s5: 40S ribosomal protein S5

s6: 40S ribosomal protein S6-A

s7: 40S ribosomal protein S7-A

s8: 40S ribosomal protein S8-A

s9: 40S ribosomal protein S9-A

c0: 40S ribosomal protein S10-A

c1: 40S ribosomal protein S11-A

c2: 40S ribosomal protein S12

c3: 40S ribosomal protein S13

c4: 40S ribosomal protein S14-B

c5: 40S ribosomal protein S15

c6: 40S ribosomal protein S16-A

c7: 40S ribosomal protein S17-A

c8: 40S ribosomal protein S18-A

c9: 40S ribosomal protein S19-A

d0: 40S ribosomal protein S20

d1: 40S ribosomal protein S21-A

d2: 40S ribosomal protein S22-A

d3: 40S ribosomal protein S23-A

d4: 40S ribosomal protein S24-A

d5: 40S ribosomal protein S25-A

d6: 40S ribosomal protein S26-B

d7: 40S ribosomal protein S27-A

d8: 40S ribosomal protein S28-A

d9: 40S ribosomal protein S29-A

e0: 40S ribosomal protein S30-A

e1: Ubiquitin-40S ribosomal protein S31

hetero molecules

Summary:

• 6.29 MDa, 159 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	6,294,772	2645
Polymers	5,951,521	159
Non-polymers	343,252	2486
Water	0

1

1: 25S ribosomal RNA

3: 5S ribosomal RNA

4: 5.8S ribosomal RNA

j: 60S ribosomal protein L2-A

k: 60S ribosomal protein L3

l: 60S ribosomal protein L4-A

m: 60S ribosomal protein L5

n: 60S ribosomal protein L6-A

o: 60S ribosomal protein L7-A

p: 60S ribosomal protein L8-A

q: 60S ribosomal protein L9-A

r: 60S ribosomal protein L10

s: 60S ribosomal protein L11-B

t: 60S ribosomal protein L13-A

u: 60S ribosomal protein L14-A

v: 60S ribosomal protein L15-A

w: 60S ribosomal protein L16-A

x: 60S ribosomal protein L17-A

y: 60S ribosomal protein L18-A

z: 60S ribosomal protein L19-A

0: 60S ribosomal protein L20-A

2: 60S ribosomal protein L21-A

5: 60S ribosomal protein L22-A

6: 60S ribosomal protein L23-A

7: 60S ribosomal protein L24-A

8: 60S ribosomal protein L25

9: 60S ribosomal protein L26-A

AA: 60S ribosomal protein L27-A

AB: 60S ribosomal protein L28

AC: 60S ribosomal protein L29

AD: 60S ribosomal protein L30

AE: 60S ribosomal protein L31-A

AF: 60S ribosomal protein L32

AG: 60S ribosomal protein L33-A

AH: 60S ribosomal protein L34-A

AI: 60S ribosomal protein L35-A

AJ: 60S ribosomal protein L36-A

AK: 60S ribosomal protein L37-A

AL: 60S ribosomal protein L38

AM: 60S ribosomal protein L39

AN: Ubiquitin-60S ribosomal protein L40

AO: 60S ribosomal protein L41-A

AP: 60S ribosomal protein L42-A

AQ: 60S ribosomal protein L43-A

i: Suppressor protein STM1

A: 18S ribosomal RNA

B: 40S ribosomal protein S0-A

C: 40S ribosomal protein S1-A

D: 40S ribosomal protein S2

E: 40S ribosomal protein S3

F: 40S ribosomal protein S4-A

G: 40S ribosomal protein S5

H: 40S ribosomal protein S6-A

I: 40S ribosomal protein S7-A

J: 40S ribosomal protein S8-A

K: 40S ribosomal protein S9-A

L: 40S ribosomal protein S10-A

M: 40S ribosomal protein S11-A

N: 40S ribosomal protein S12

O: 40S ribosomal protein S13

P: 40S ribosomal protein S14-B

Q: 40S ribosomal protein S15

R: 40S ribosomal protein S16-A

S: 40S ribosomal protein S17-A

T: 40S ribosomal protein S18-A

U: 40S ribosomal protein S19-A

V: 40S ribosomal protein S20

W: 40S ribosomal protein S21-A

X: 40S ribosomal protein S22-A

Y: 40S ribosomal protein S23-A

Z: 40S ribosomal protein S24-A

a: 40S ribosomal protein S25-A

b: 40S ribosomal protein S26-B

c: 40S ribosomal protein S27-A

d: 40S ribosomal protein S28-A

e: 40S ribosomal protein S29-A

f: 40S ribosomal protein S30-A

g: Ubiquitin-40S ribosomal protein S31

h: Guanine nucleotide-binding protein subunit beta-like protein

hetero molecules

Summary:

• defined by author
• 3.13 MDa, 79 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	3,134,510	1272
Polymers	2,969,078	79
Non-polymers	165,431	1193
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

AR: 25S ribosomal RNA

AS: 5S ribosomal RNA

AT: 5.8S ribosomal RNA

CD: 60S ribosomal protein L2-A

CE: 60S ribosomal protein L3

CF: 60S ribosomal protein L4-A

CG: 60S ribosomal protein L5

CH: 60S ribosomal protein L6-A

CI: 60S ribosomal protein L7-A

CJ: 60S ribosomal protein L8-A

CK: 60S ribosomal protein L9-A

CL: 60S ribosomal protein L10

CM: 60S ribosomal protein L11-B

CN: 60S ribosomal protein L13-A

CO: 60S ribosomal protein L14-A

CP: 60S ribosomal protein L15-A

CQ: 60S ribosomal protein L16-A

CR: 60S ribosomal protein L17-A

CS: 60S ribosomal protein L18-A

CT: 60S ribosomal protein L19-A

CU: 60S ribosomal protein L20-A

CV: 60S ribosomal protein L21-A

CW: 60S ribosomal protein L22-A

CX: 60S ribosomal protein L23-A

CY: 60S ribosomal protein L24-A

CZ: 60S ribosomal protein L25

DA: 60S ribosomal protein L26-A

DB: 60S ribosomal protein L27-A

DC: 60S ribosomal protein L28

DD: 60S ribosomal protein L29

DE: 60S ribosomal protein L30

DF: 60S ribosomal protein L31-A

DG: 60S ribosomal protein L32

DH: 60S ribosomal protein L33-A

DI: 60S ribosomal protein L34-A

DJ: 60S ribosomal protein L35-A

DK: 60S ribosomal protein L36-A

DL: 60S ribosomal protein L37-A

DM: 60S ribosomal protein L38

DN: 60S ribosomal protein L39

DO: Ubiquitin-60S ribosomal protein L40

DP: 60S ribosomal protein L41-A

DQ: 60S ribosomal protein L42-A

DR: 60S ribosomal protein L43-A

p0: 60S acidic ribosomal protein P0

sM: Suppressor protein STM1

sR: 18S ribosomal RNA

Rb: Guanine nucleotide-binding protein subunit beta-like protein

s0: 40S ribosomal protein S0-A

s1: 40S ribosomal protein S1-A

s2: 40S ribosomal protein S2

s3: 40S ribosomal protein S3

s4: 40S ribosomal protein S4-A

s5: 40S ribosomal protein S5

s6: 40S ribosomal protein S6-A

s7: 40S ribosomal protein S7-A

s8: 40S ribosomal protein S8-A

s9: 40S ribosomal protein S9-A

c0: 40S ribosomal protein S10-A

c1: 40S ribosomal protein S11-A

c2: 40S ribosomal protein S12

c3: 40S ribosomal protein S13

c4: 40S ribosomal protein S14-B

c5: 40S ribosomal protein S15

c6: 40S ribosomal protein S16-A

c7: 40S ribosomal protein S17-A

c8: 40S ribosomal protein S18-A

c9: 40S ribosomal protein S19-A

d0: 40S ribosomal protein S20

d1: 40S ribosomal protein S21-A

d2: 40S ribosomal protein S22-A

d3: 40S ribosomal protein S23-A

d4: 40S ribosomal protein S24-A

d5: 40S ribosomal protein S25-A

d6: 40S ribosomal protein S26-B

d7: 40S ribosomal protein S27-A

d8: 40S ribosomal protein S28-A

d9: 40S ribosomal protein S29-A

e0: 40S ribosomal protein S30-A

e1: Ubiquitin-40S ribosomal protein S31

hetero molecules

Summary:

• defined by author
• 3.16 MDa, 80 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	3,160,262	1373
Polymers	2,982,442	80
Non-polymers	177,820	1293
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	304.800, 288.200, 437.680
Angle α, β, γ (deg.)	90.000, 98.710, 90.000
Int Tables number	4
Space group name H-M	P1211

Components

RNA chain , 4 types, 8 molecules 1 AR 3 AS 4 AT A sR

#1: RNA chain

1 AR 25S ribosomal RNA /

Details:

Mass: 1017859.125 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast)

#2: RNA chain

3 AS 5S ribosomal RNA /

Details:

Mass: 38951.105 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1039024011

#3: RNA chain

4 AT 5.8S ribosomal RNA /

Details:

Mass: 50682.922 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1043567390

#48: RNA chain

A sR 18S ribosomal RNA /

Details:

Mass: 579761.938 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 874346701

60S ribosomal protein ... , 40 types, 80 molecules j CD k CE l CF m CG n CH o CI p CJ q CK r CL s CM t CN u CO v CP w CQ x CR ...

#4: Protein

j CD 60S ribosomal protein L2-A / Ribosome / L5 / RP8 / YL6

Details:

Mass: 27217.289 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL2A, RPL5B, YFR031C-A, YFR031BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX45

#5: Protein

k CE 60S ribosomal protein L3 / / Maintenance of killer protein 8 / RP1 / Trichodermin resistance protein / YL1

Details:

Mass: 43719.602 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL3, MAK8, TCM1, YOR063W, YOR29-14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14126

#6: Protein

l CF 60S ribosomal protein L4-A / Ribosome / L2 / RP2 / YL2

Details:

Mass: 39027.926 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL4A, RPL2, RPL2A, YBR031W, YBR0315 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P10664

#7: Protein

m CG 60S ribosomal protein L5 / / L1 / L1a / Ribosomal 5S RNA-binding protein / YL3

Details:

Mass: 33633.633 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL5, RPL1, RPL1A, YPL131W, LPI14W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26321

#8: Protein

n CH 60S ribosomal protein L6-A / Ribosome / L17 / RP18 / YL16

Details:

Mass: 19869.369 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL6A, RPL17A, YL16A, YML073C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02326

#9: Protein

o CI 60S ribosomal protein L7-A / Ribosome / L6 / RP11 / YL8

Details:

Mass: 25240.377 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL7A, RPL6A, RPL8A, YL8A, YGL076C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05737

#10: Protein

p CJ 60S ribosomal protein L8-A / Ribosome / L4 / L4-2 / L7a-1 / Maintenance of killer protein 7 / RP6 / YL5

Details:

Mass: 25814.998 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL8A, MAK7, RPL4A, YHL033C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P17076

#11: Protein

q CK 60S ribosomal protein L9-A / Ribosome / L8 / RP24 / YL11

Details:

Mass: 21605.061 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL9A, RPL8A, RPL9, YGL147C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05738

#12: Protein

r CL 60S ribosomal protein L10 / / L9 / Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein

Details:

Mass: 25279.271 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL10, GRC5, QSR1, YLR075W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41805

#13: Protein

s CM 60S ribosomal protein L11-B / Ribosome / L16 / RP39 / YL22

Details:

Mass: 19266.082 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL11B, RP39B, RPL16B, YGR085C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E757

#14: Protein

t CN 60S ribosomal protein L13-A / Ribosome

Details:

Mass: 21885.234 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL13A, YDL082W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12690

#15: Protein

u CO 60S ribosomal protein L14-A / Ribosome

Details:

Mass: 14976.794 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL14A, YKL006W, YKL153 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36105

#16: Protein

v CP 60S ribosomal protein L15-A / Ribosome / L13 / RP15R / YL10 / YP18

Details:

Mass: 24351.162 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL15A, RPL10A, RPL13A, YL10A, YLR029C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05748

#17: Protein

w CQ 60S ribosomal protein L16-A / Ribosome / L13a / L21 / RP22 / YL15

Details:

Mass: 22028.951 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL16A, RPL13, RPL21A, YIL133C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26784

#18: Protein

x CR 60S ribosomal protein L17-A / Ribosome / L20A / YL17

Details:

Mass: 20458.322 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL17A, RPL17, RPL20A, YKL180W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05740

#19: Protein

y CS 60S ribosomal protein L18-A / Ribosome / RP28

Details:

Mass: 20478.059 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL18A, RP28A, YOL120C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX49

#20: Protein

z CT 60S ribosomal protein L19-A / Ribosome / L23 / RP15L / RP33 / YL14

Details:

Mass: 21631.121 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL19A, RPL23A, YL14A, YBR084C-A, YBR084BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX82

#21: Protein

0 CU 60S ribosomal protein L20-A / Ribosome / L18a

Details:

Mass: 20478.852 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL20A, RPL18A, RPL18A2, YMR242C, YM9408.04C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX23

#22: Protein

2 CV 60S ribosomal protein L21-A / Ribosome

Details:

Mass: 18148.066 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL21A, URP1, YBR191W, YBR1401 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02753

#23: Protein

5 CW 60S ribosomal protein L22-A / Ribosome / L1c / RP4 / YL31

Details:

Mass: 11263.863 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL22A, YLR061W, L2168 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05749

#24: Protein

6 CX 60S ribosomal protein L23-A / Ribosome / L17a / YL32

Details:

Mass: 14362.755 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL23A, RPL17A, RPL17AA, YBL087C, YBL0713 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX41

#25: Protein

7 CY 60S ribosomal protein L24-A / Ribosome / L30 / RP29 / YL21

Details:

Mass: 11369.424 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL24A, RP29, RPL30A, YGL031C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04449

#26: Protein

8 CZ 60S ribosomal protein L25 / / RP16L / YL25 / YP42'

Details:

Mass: 13771.176 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL25, YOL127W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04456

#27: Protein

9 DA 60S ribosomal protein L26-A / Ribosome / L33 / YL33

Details:

Mass: 14134.588 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL26A, RPL26, RPL33A, YLR344W, L8300.4 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05743

#28: Protein

AA DB 60S ribosomal protein L27-A / Ribosome

Details:

Mass: 15437.166 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL27A, RPL27, YHR010W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C2H6

#29: Protein

AB DC 60S ribosomal protein L28 / / L27a / L29 / RP44 / RP62 / YL24

Details:

Mass: 16630.471 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL28, CYH2, YGL103W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P02406

#30: Protein

AC DD 60S ribosomal protein L29 / / YL43

Details:

Mass: 6560.688 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL29, YL43, YFR032C-A, YFR032BW / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05747

#31: Protein

AD DE 60S ribosomal protein L30 / / L32 / RP73 / YL38

Details:

Mass: 10558.344 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL30, RPL32, YGL030W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14120

#32: Protein

AE DF 60S ribosomal protein L31-A / Ribosome / L34 / YL28

Details:

Mass: 12649.754 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL31A, RPL34, RPL34A, YDL075W, D2478 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C2H8

#33: Protein

AF DG 60S ribosomal protein L32 /

Details:

Mass: 14478.054 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL32, YBL092W, YBL0838 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38061

#34: Protein

AG DH 60S ribosomal protein L33-A / Ribosome / L37 / RP47 / YL37

Details:

Mass: 12045.935 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL33A, RPL37A, YPL143W, LPI4W, P2625 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05744

#35: Protein

AH DI 60S ribosomal protein L34-A / Ribosome

Details:

Mass: 12608.831 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL34A, YER056C-A, YER056BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P87262

#36: Protein

AI DJ 60S ribosomal protein L35-A / Ribosome

Details:

Mass: 13811.444 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL35A, SOS1, YDL191W, D1249 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX84

#37: Protein

AJ DK 60S ribosomal protein L36-A / Ribosome / L39 / YL39

Details:

Mass: 11020.063 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL36A, RPL39A, YMR194W, YM9646.06 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05745

#38: Protein

AK DL 60S ribosomal protein L37-A / Ribosome / L43 / YL35 / YP55

Details:

Mass: 9746.199 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL37A, RPL35A, YLR185W, L9470.6 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P49166

#39: Protein

AL DM 60S ribosomal protein L38 /

Details:

Mass: 8714.363 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL38, YLR325C, L8543.2 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P49167

#40: Protein/peptide

AM DN 60S ribosomal protein L39 / / L46 / YL40

Details:

Mass: 6227.444 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL39, RPL46, SPB2, YJL189W, J0360 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04650

#42: Protein/peptide

AO DP 60S ribosomal protein L41-A / Ribosome / L47 / YL41

Details:

Mass: 3354.243 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL41A, RPL47A, YL41A, YDL184C, D1290 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX86

#43: Protein

AP DQ 60S ribosomal protein L42-A / Ribosome / L41 / YL27 / YP44

Details:

Mass: 12115.462 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL42A, RPL41A, SCL41A, YNL162W, N1722 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX27

#44: Protein

AQ DR 60S ribosomal protein L43-A / Ribosome / L37a / YL35

Details:

Mass: 9981.756 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL43A, YPR043W, YP9499.02 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX25

Protein , 3 types, 5 molecules AN DO p0 h Rb

#41: Protein

AN DO Ubiquitin-60S ribosomal protein L40

Details:

Mass: 6032.321 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL40A, UBI1, YIL148W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CH08

#46: Protein

p0 60S acidic ribosomal protein P0 / / A0 / L10E

Details:

Mass: 24044.625 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPP0, L10E, RPA0, RPL10E, RPLA0, YLR340W, L8300.8 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05317

#81: Protein

h Rb Guanine nucleotide-binding protein subunit beta-like protein / Receptor for activated C kinase / Receptor of activated protein kinase C 1 / RACK1

Details:

Mass: 34710.023 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: ASC1, CPC2, YMR116C, YM9718.15C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38011

Suppressor protein ... , 2 types, 2 molecules i sM

#45: Protein

i Suppressor protein STM1 / 3BP1 / GU4 nucleic-binding protein 2 / G4p2 protein / POP2 multicopy suppressor protein 4 / Ribosomal subunits association factor / AF / TOM1 suppressor protein 1 / Triplex-binding protein 1

Details:

Mass: 18223.033 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: STM1, MPT4, STO1, YLR150W, L9634.1 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39015

#47: Protein

sM Suppressor protein STM1 / 3BP1 / GU4 nucleic-binding protein 2 / G4p2 protein / POP2 multicopy suppressor protein 4 / Ribosomal subunits association factor / AF / TOM1 suppressor protein 1 / Triplex-binding protein 1

Details:

Mass: 10310.914 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: STM1, MPT4, STO1, YLR150W, L9634.1 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39015

40S ribosomal protein ... , 32 types, 62 molecules B s0 C s1 D s2 E s3 F s4 G s5 H s6 I s7 J s8 K s9 L c0 M c1 N c2 O c3 P c4 ...

#49: Protein

B s0 40S ribosomal protein S0-A / Ribosome / Nucleic acid-binding protein NAB1A

Details:

Mass: 22811.074 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS0A, NAB1, NAB1A, YST1, YGR214W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32905

#50: Protein

C s1 40S ribosomal protein S1-A / Ribosome / RP10A

Details:

Mass: 24498.365 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS1A, PLC1, RPS10A, YLR441C, L9753.9 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P33442

#51: Protein

D s2 40S ribosomal protein S2 / / Omnipotent suppressor protein SUP44 / RP12 / S4 / YS5

Details:

Mass: 23212.979 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS2, RPS4, SUP38, SUP44, YGL123W, G2893 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P25443

#52: Protein

E s3 40S ribosomal protein S3 / / RP13 / YS3

Details:

Mass: 24702.791 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS3, SUF14, YNL178W, N1653 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05750

#53: Protein

F s4 40S ribosomal protein S4-A / Ribosome / RP5 / S7 / YS6

Details:

Mass: 29338.133 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS4A, RPS7B, YJR145C, J2186 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX35

#54: Protein

G s5 40S ribosomal protein S5 / / RP14 / S2 / YS8

Details:

Mass: 22908.338 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS5, RPS2, YJR123W, J2045 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26783

#55: Protein

H s6 40S ribosomal protein S6-A / Ribosome / RP9 / S10 / YS4

Details:

Mass: 25895.072 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS6A, RPS101, RPS10B, YPL090C, LPG18C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX37

#56: Protein

I s7 40S ribosomal protein S7-A / Ribosome / RP30 / RP40

Details:

Mass: 21158.648 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS7A, RPS30, YOR096W, YOR3177W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26786

#57: Protein

J s8 40S ribosomal protein S8-A / Ribosome / RP19 / S14 / YS9

Details:

Mass: 22406.609 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS8A, RPS14A, YBL072C, YBL06.05, YBL0613 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX39

#58: Protein

K s9 40S ribosomal protein S9-A / Ribosome / RP21 / S13 / YP28 / YS11

Details:

Mass: 21210.662 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS9A, RPS13A, YS11A, YPL081W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: O13516

#59: Protein

L c0 40S ribosomal protein S10-A / Ribosome

Details:

Mass: 12757.445 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS10A, YOR293W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q08745

#60: Protein

M c1 40S ribosomal protein S11-A / Ribosome / RP41 / S18 / YS12

Details:

Mass: 17654.738 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS11A, RPS18A, YDR025W, YD9813.03 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX47

#61: Protein

N c2 40S ribosomal protein S12 /

Details:

Mass: 15488.631 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS12, YOR369C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P48589

#62: Protein

O c3 40S ribosomal protein S13 / / S27a / YS15

Details:

Mass: 16928.748 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS13, RPS13C, YDR064W, D4252, YD9609.18 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05756

#63: Protein

P c4 40S ribosomal protein S14-B / Ribosome / RP59B

Details:

Mass: 13560.528 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS14B, CRY2, YJL191W, J0354 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39516

#64: Protein

Q c5 40S ribosomal protein S15 / / RIG protein / RP52 / S21 / YS21

Details:

Mass: 15232.891 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS15, RPS21, YOL040C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q01855

#65: Protein

R c6 40S ribosomal protein S16-A / Ribosome / RP61R

Details:

Mass: 15746.292 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS16A, RP61R, YMR143W, YM9375.12 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX51

#66: Protein

S 40S ribosomal protein S17-A / Ribosome / RP51A

Details:

Mass: 14268.545 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS17A, RP51A, YML024W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P02407, UniProt: P14127*PLUS

#67: Protein

T c8 40S ribosomal protein S18-A / Ribosome

Details:

Mass: 16940.443 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS18A, YDR450W, D9461.35 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX55

#68: Protein

U c9 40S ribosomal protein S19-A / Ribosome / RP55A / S16a / YP45 / YS16A

Details:

Mass: 15810.930 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS19A, RP55A, RPS16AA, YOL121C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P07280

#69: Protein

V d0 40S ribosomal protein S20 /

Details:

Mass: 12575.547 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS20, URP2, YHL015W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38701

#70: Protein

W d1 40S ribosomal protein S21-A / Ribosome / S26 / YS25

Details:

Mass: 9758.829 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS21A, RPS25, RPS25A, RPS26A, YKR057W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C0V8

#71: Protein

X d2 40S ribosomal protein S22-A / Ribosome / RP50 / S24 / YP58 / YS22

Details:

Mass: 14518.867 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS22A, RPS24, RPS24A, YJL190C, J0355 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C0W1

#72: Protein

Y d3 40S ribosomal protein S23-A / Ribosome / RP37 / S28 / YS14

Details:

Mass: 15942.699 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS23A, RPS28A, YGR118W, G6178 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX29

#73: Protein

Z d4 40S ribosomal protein S24-A / Ribosome / RP50

Details:

Mass: 15231.650 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS24A, RPS24EA, YER074W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX31

#74: Protein

a d5 40S ribosomal protein S25-A / Ribosome / RP45 / S31 / YS23

Details:

Mass: 8001.377 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS25A, RPS31A, YGR027C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E792

#75: Protein

b d6 40S ribosomal protein S26-B / Ribosome

Details:

Mass: 11022.989 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS26B, YER131W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39939

#76: Protein

c d7 40S ribosomal protein S27-A / Ribosome / RP61 / YS20

Details:

Mass: 8762.195 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS27A, YKL156W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P35997

#77: Protein

d d8 40S ribosomal protein S28-A / Ribosome / S33 / YS27

Details:

Mass: 7116.281 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS28A, RPS33, RPS33A, YOR167C, O3600 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E7X9

#78: Protein

e d9 40S ribosomal protein S29-A / Ribosome / S36 / YS29

Details:

Mass: 6335.303 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS29A, RPS36A, YS29A, YLR388W, L8084.11 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41057

#79: Protein

f e0 40S ribosomal protein S30-A / Ribosome

Details:

Mass: 7006.346 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS30A, YLR287C-A, L8003.23, YLR287BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX33

#82: Protein

c7 40S ribosomal protein S17-A / Ribosome / RP51A

Details:

Mass: 13897.153 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS17A, RP51A, YML024W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P02407

Ubiquitin-40S ribosomal protein ... , 2 types, 2 molecules g e1

#80: Protein

g Ubiquitin-40S ribosomal protein S31

Details:

Mass: 8101.675 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS31, RPS37, UBI3, YLR167W, L9470.14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05759

#83: Protein

e1 Ubiquitin-40S ribosomal protein S31

Details:

Mass: 5704.610 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS31, RPS37, UBI3, YLR167W, L9470.14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05759

Non-polymers , 4 types, 2486 molecules

#84: Chemical

1-3401 1-3402 1-3403 1-3404 1-3405 1-3406 1-3407 1-3408 1-3409 1-3410 1-3411 1-3412 1-3413 1-3414 1-3415 1-3416 1-3417 1-3418 1-3419 1-3420 ...
ChemComp-OHX / osmium (III) hexammine / osmium(6+) hexaazanide

Details:

Mass: 286.365 Da / Num. of mol.: 1074 / Source method: obtained synthetically / Formula: H₁₂N₆Os

#85: Chemical

1-3732 1-3733 1-3734 1-3735 1-3736 1-3737 1-3738 1-3739 1-3740 1-3741 1-3742 1-3743 1-3744 1-3745 1-3746 1-3747 1-3748 1-3749 1-3750 1-3751 ...
ChemComp-MG / MAGNESIUM ION

Details:

Mass: 24.305 Da / Num. of mol.: 1394 / Source method: obtained synthetically / Formula: Mg

#86: Chemical

1-4210 AR-4246 ChemComp-7AL / Chlorolissoclimide

Details:

Mass: 383.910 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₂₀H₃₀ClNO₄

#87: Chemical

AK-101 AN-500 AP-501 AQ-501 DL-102 DO-201 DQ-202 DR-501 b-102 c-101 e-103 g-501 d6-103 d7-101 d9-103 e1-501
ChemComp-ZN / ZINC ION

Details:

Mass: 65.409 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Zn

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.22 ų/Da / Density % sol: 61.74 %
• Crystal grow: Temperature: 277.15 K / Method: vapor diffusion, hanging drop / pH: 7 ; Details: Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 1.148 Å
• Detector: Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 6, 2015
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1.148 Å / Relative weight: 1
• Reflection: Resolution: 3→100 Å / Num. obs: 1484770 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 9 % / B_iso Wilson estimate: 62.26 Ų / CC_1/2: 0.997 / R_merge(I) obs: 0.204 / R_rim(I) all: 0.216 / Χ²: 1.034 / Net I/σ(I): 10.63 / Num. measured all: 13350896

Reflection shell

Diffraction-ID: 1 / Rejects: 0

Resolution (Å)	Rmerge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. possible	Num. unique obs	CC1/2	Rrim(I) all	% possible all
3-3.1	1.124	1.39	752990	138792	138249	0.58	1.243	99.6
3.1-3.25	0.794	2.39	1198156	177483	177441	0.783	0.861	100
3.25-3.5	0.56	4.02	1829452	232502	232473	0.9	0.599	100
3.5-4	0.29	7.71	2533200	308427	308406	0.971	0.31	100
4-4.5	0.165	12.61	1541125	186406	186395	0.988	0.176	100
4.5-5	0.124	16.01	975695	119348	119333	0.993	0.133	100
5-6	0.219	18.98	1659204	135435	135422	0.994	0.228	100
6-8	0.188	22.37	1665493	107751	107750	0.996	0.195	100
8-10	0.111	28.55	588021	38518	38512	0.998	0.115	100
10-15	0.077	34.93	432469	28595	28589	0.998	0.079	100
15-20	0.066	37.53	99905	7011	7009	0.999	0.068	100
20-25	0.051	45.63	38165	2531	2532	0.999	0.053	100
25-50	0.04	51.75	34185	2349	2349	0.999	0.041	100
50-75	0.036	47.73	2528	250	246	0.999	0.038	98.4
75-100	0.021	37.88	273	61	51	1	0.023	83.6

Processing

Software

Name	Version	Classification
PHENIX		refinement
XSCALE		data scaling
PDB_EXTRACT	3.2	data extraction
XDS		data reduction
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 4V88

4v88

Resolution: 3→99.856 Å / SU ML: 0.36 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 22.84 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2267	29477	1.99 %
Rwork	0.1938	1455100	-
obs	0.1944	1484577	99.95 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso max: 298.35 Ų / B_iso mean: 74.4126 Ų / B_iso min: 14.76 Ų

Refinement step

Cycle: final / Resolution: 3→99.856 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	178135	222512	8974	0	409621
Biso mean	-	-	124.8	-	-
Num. residues	-	-	-	-	33045

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.006	436671
X-RAY DIFFRACTION	f_angle_d	1.043	647820
X-RAY DIFFRACTION	f_chiral_restr	0.044	79239
X-RAY DIFFRACTION	f_plane_restr	0.006	41520
X-RAY DIFFRACTION	f_dihedral_angle_d	17.305	194593

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Num. reflection all	% reflection obs (%)
3-3.0341	0.3539	934	0.3202	47861	48795	99
3.0341-3.0698	0.3452	984	0.3009	48374	49358	100
3.0698-3.1072	0.3218	964	0.2893	48532	49496	100
3.1072-3.1466	0.3082	983	0.2839	48357	49340	100
3.1466-3.188	0.3252	978	0.2848	48346	49324	100
3.188-3.2316	0.3124	985	0.2648	48457	49442	100
3.2316-3.2778	0.2713	958	0.2376	48470	49428	100
3.2778-3.3267	0.2724	1004	0.2312	48368	49372	100
3.3267-3.3787	0.2648	962	0.218	48444	49406	100
3.3787-3.4341	0.2412	996	0.2088	48456	49452	100
3.4341-3.4933	0.2365	974	0.2054	48377	49351	100
3.4933-3.5569	0.2377	982	0.1974	48481	49463	100
3.5569-3.6253	0.2369	962	0.1956	48445	49407	100
3.6253-3.6993	0.2281	1040	0.1955	48408	49448	100
3.6993-3.7797	0.2267	1003	0.1881	48406	49409	100
3.7797-3.8677	0.209	1028	0.1763	48422	49450	100
3.8677-3.9644	0.2106	967	0.1724	48537	49504	100
3.9644-4.0716	0.2055	947	0.1682	48544	49491	100
4.0716-4.1914	0.2094	1020	0.1683	48429	49449	100
4.1914-4.3267	0.2028	947	0.1661	48546	49493	100
4.3267-4.4813	0.2012	983	0.1634	48542	49525	100
4.4813-4.6607	0.1893	938	0.1607	48681	49619	100
4.6607-4.8728	0.1923	1018	0.1589	48438	49456	100
4.8728-5.1297	0.1948	959	0.1621	48601	49560	100
5.1297-5.4511	0.1945	1005	0.161	48599	49604	100
5.4511-5.872	0.193	980	0.1635	48633	49613	100
5.872-6.4628	0.2071	974	0.1743	48650	49624	100
6.4628-7.3977	0.2168	1032	0.1849	48704	49736	100
7.3977-9.3193	0.2351	967	0.201	48892	49859	100
9.3193-99.9116	0.2247	1003	0.2226	49100	50103	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.1267	0.0095	-0.0451	0.0878	0.0075	0.146	0.0013	0.0153	-0.0179	0.0022	-0.0358	-0.037	0.0002	0.0169	-0.2207	0.1639	-0.023	-0.0012	0.2114	0.0472	0.1666	68.805	-1.3616	48.1469
2	0.1083	0.0258	0.1887	0.1051	0.0488	0.1812	-0.0215	0.1073	0.1033	-0.0805	0.004	0.249	-0.1212	-0.262	-0.1556	0.4344	0.2441	-0.007	0.4462	0.1483	0.4126	12.9811	67.6707	26.2738
3	0.1498	0.0403	0.0313	0.1544	0.0643	0.205	0.0292	-0.1436	-0.0158	0.0697	-0.0287	-0.1349	0.0668	0.1878	-0.0183	0.249	-0.0009	-0.0577	0.5156	0.1333	0.384	115.7958	-7.1976	82.879
4	0.0584	0.0224	0.0317	0.0905	0.0522	0.0825	-0.0164	0.0857	0.0871	-0.1225	-0.0224	-0.0606	-0.1292	0.0698	-0.0643	0.1884	0.0009	0.0816	0.3823	0.1192	0.2969	123.9082	4.5533	16.6878
5	0.1239	-0.0305	0.0426	0.0855	0.0289	0.0829	-0.0233	0.1032	-0.1672	-0.0086	-0.0473	-0.0358	0.1154	-0.104	-0.0185	0.3635	-0.1441	0.1034	0.2464	-0.101	0.3305	33.6443	-61.0482	37.7468
6	0.1183	0.0654	-0.0828	0.2011	-0.0302	0.1505	0.0797	-0.122	-0.0522	0.1151	-0.0674	-0.0592	-0.0494	0.054	-0.012	0.2795	-0.094	-0.0309	0.3381	-0.02	0.0592	58.5401	25.1525	103.4542
7	0.0362	-0.0644	0.0228	0.1173	0.0209	0.0556	-0.0807	0.0581	0.2659	0.0187	-0.0537	0.0661	-0.2303	-0.1629	-0.1279	0.6924	0.3175	-0.1024	0.2303	0.194	0.5379	26.3643	94.0226	28.9328
8	0.0319	-0.0325	0.0112	0.056	-0.0492	0.0544	-0.0002	-0.0154	-0.0303	0.1594	-0.0319	-0.0107	-0.0974	0.0013	-0.0011	0.341	-0.1146	0.0747	0.4694	-0.0371	0.2332	-3.9834	-11.9568	106.8693
9	0.0481	-0.0529	0.0094	0.0476	-0.0094	0.0403	-0.0208	0.0767	0.117	0.0756	0.007	0.0603	-0.0529	-0.0412	0	0.2786	0.0446	0.0246	0.3626	-0.0379	0.2583	4.9733	31.7507	77.7671
10	0.0694	-0.0493	-0.0043	0.0746	0.0202	0.0197	0.0903	0.0011	0.0139	-0.0694	-0.0425	-0.1986	-0.1041	0.166	-0.0015	0.3731	-0.2243	0.0352	0.7539	0.0708	0.6862	155.4902	40.5041	53.8519
11	0.0419	0.02	0.0378	0.0272	0.0051	0.0476	-0.0903	0.0588	-0.0548	-0.0475	0.0227	0.0766	0.1128	-0.0884	-0.0002	0.4228	-0.1729	0.015	0.7408	-0.2011	0.3588	-11.8101	-32.7871	18.689
12	0.0207	-0.0066	-0.0232	0.0317	0.0031	0.0801	0.026	0.1201	0.0042	-0.1543	0.0252	0.0572	-0.0639	-0.0763	0.0315	0.3304	0.0601	-0.0922	0.5458	0.0867	0.1732	10.5412	27.6599	13.2027
13	0.0057	-0.0147	-0.0069	0.0271	-0.0004	0.0269	-0.0849	0.1862	0.0739	-0.1657	0.0015	0.0481	-0.1385	0.07	-0.0002	0.8711	0.112	-0.03	0.7108	0.2491	0.4218	31.5347	79.5538	-7.7667
14	0.0403	0.0229	0.0029	0.0265	-0.0123	0.0002	0.1102	-0.133	0.0581	0.0735	-0.0044	-0.0771	-0.0512	0.0302	0.1463	0.2731	-0.2467	-0.0717	0.3847	-0.0213	0.3002	99.6922	55.6382	88.3863
15	0.01	0.0236	0.0109	0.017	-0.0039	0.0267	-0.0336	0.0152	0.0311	0.0748	-0.0711	0.0545	0.0534	-0.1456	-0.0002	0.2469	-0.0845	0.0501	0.4953	-0.0088	0.3174	-26.1683	-14.0134	65.2781
16	0.0481	-0.0256	-0.0048	0.0429	0.0098	0.0414	0.0154	-0.0354	0.0069	-0.0194	-0.0351	-0.0702	-0.0528	0.041	-0.0403	0.1669	-0.1724	-0.0093	0.4232	0.1012	0.318	120.1319	32.7415	62.1195
17	0.0362	0.0143	-0.0189	0.0034	-0.0058	0.0236	-0.0468	0.0373	-0.0072	0.0048	-0.0013	0.044	0.0729	-0.1281	-0.0036	0.2424	-0.1363	0.0513	0.3623	-0.0918	0.1919	3.6153	-29.8801	57.2522
18	0.0305	-0.0436	0.0398	0.0809	-0.0601	0.0669	-0.056	-0.0417	-0.0479	0.0793	-0.0476	0.0373	0.0453	0.0451	-0	0.3534	-0.0382	0.0191	0.3068	0.1145	0.3272	56.0984	-40.2703	83.1809
19	0.0433	-0.0568	-0.015	0.0764	-0.0087	0.0109	0.0302	-0.0011	0.0699	0.0083	-0.0173	-0.006	-0.0419	0.0297	0	0.3495	-0.0846	0.0326	0.2195	-0.0211	0.208	52.4976	50.3993	90.5865
20	0.0131	-0.0457	-0.0228	-0.0065	0.0057	0.036	0.0315	0.0671	-0.0927	0.0065	0.0085	-0.1218	0.078	-0.0321	-0.0007	0.4314	0.1657	0.1006	0.5188	-0.0042	0.6205	122.3856	-57.6024	10.7237
21	0.0791	0.0045	-0.0744	0.0175	-0.0353	0.0257	-0.0578	0.0616	0.0513	-0.0865	0.0294	0.0067	0.0599	-0.1679	-0.0001	0.2673	0.0291	-0.0352	0.5474	-0.0077	0.3095	-12.3148	14.6086	47.5174
22	0.0041	-0.0056	-0.0021	0.0579	-0.0106	0.0152	0.0377	0.0551	0.0926	-0.0623	-0.0246	0.0606	-0.0461	-0.0927	-0	0.364	0.1043	-0.0142	0.3713	0.0459	0.3621	22.0983	55.5701	50.4441
23	0.0009	0.0016	0.0019	0.0067	-0.001	0.0012	0.0307	-0.0316	-0.0804	0.0214	-0.0045	0.0175	0.0208	0.0081	0	0.6947	0.2141	-0.0435	0.5362	0.0325	1.0076	114.2976	-89.5461	42.2746
24	0.0096	0.0074	0.0101	0.0256	-0.0061	0.0106	-0.0447	0.0701	-0.0716	-0.0377	-0.0103	0.0877	0.0337	-0.0473	0.0001	0.4808	-0.0232	0.0796	0.5092	-0.1233	0.3598	49.2944	-45.16	-0.3534
25	0.0078	0.002	-0.0136	0.0176	-0.0181	0.0308	0.0346	0.0179	0.0114	-0.0968	-0.0021	0.0292	0.0639	0.0024	0	0.585	-0.0458	0.0774	0.3963	-0.0826	0.5515	57.5451	-80.9768	3.6709
26	0.0699	-0.0465	0.0118	0.0284	-0.0263	0.0487	0.0049	0.0189	0.0717	0.0611	0.0202	-0.0902	-0.0362	0.1303	0.0032	0.2975	0.088	-0.0822	0.5807	0.1785	0.5441	134.3379	-24.5203	74.5396
27	0.0144	-0.0005	0.0038	0.0115	-0	0.0029	-0.0305	-0.098	-0.0238	0.0647	0.0336	-0.0083	-0.002	0.0581	0.0009	0.363	-0.0588	-0.167	0.6017	0.1932	0.1925	97.705	-11.3475	118.1043
28	0.0164	-0.0038	-0.0012	0.0001	0.0036	0.0048	-0.0434	0.004	-0.0069	0.0116	-0.0266	-0.0732	-0.009	0.0684	-0	0.3314	0.1251	0.1815	0.9315	0.0399	1.0004	173.8148	-26.9453	19.5728
29	0.0771	0.025	-0.0569	0.0426	-0.0359	0.0026	0.0545	-0.0352	0.0926	0.0236	-0.0312	0.0118	-0.1277	0.0364	-0.0004	0.2919	-0.1196	-0.0165	0.3018	-0.014	0.23	72.9275	54.7394	79.2622
30	0.0111	-0.0179	0.0186	0.0077	-0.0064	0.0237	0.0142	0.0197	0.0027	0.0034	0.0003	0.0069	-0.0573	0.0617	-0	0.3651	0.0495	0.0073	0.3498	0.0572	0.2869	42.2133	56.8558	63.731
31	0.0067	0.0023	0	0.0019	0.0009	0.0064	-0.064	0.0097	0.0529	-0.0728	-0.0235	0.0066	-0.0249	-0.0011	-0	0.4426	0.0804	0.2056	0.8659	-0.0096	0.6798	156.6656	-22.7511	-8.3252
32	0.0168	0.0019	0.0117	0.011	-0.0014	0.0059	-0.0235	-0.0144	-0.052	0.0029	-0.0129	-0.0246	0.0202	0.0007	-0	0.5345	0.0014	0.058	0.3231	0.0554	0.5318	73.8283	-71.9187	56.1765
33	0.0399	0.0264	0.0066	0.0272	-0.0098	0.0209	-0.0121	-0.1407	-0.0437	0.0857	-0.02	-0.0162	0.0341	-0.011	-0	0.2649	-0.0772	-0.0135	0.3593	0.0163	0.142	43.4592	0.4606	98.0546
34	0.025	0.0142	-0.0117	0.0088	0.0066	0.0211	-0.0531	0.0401	0.0236	0.0777	-0.0454	-0.0696	0.0136	0.019	0	0.2794	-0.0574	0.0472	0.4174	0.0245	0.2316	9.4778	-14.1094	84.2087
35	0.0214	-0.023	-0.0205	0.0503	0.055	0.0265	0.0252	-0.0095	-0.0204	0.0057	-0.0066	-0.0541	0.0415	0.0192	0	0.2827	0.1223	0.0767	0.5991	0.1268	0.5681	142.499	-26.971	28.6627
36	0.0379	-0.0058	-0.008	0.0032	-0.0092	0.0714	-0.0165	-0.0241	0.0233	0.0802	0.0701	-0.0721	-0.0294	0.1803	0.0014	0.2672	-0.0727	-0.1314	0.6401	0.0586	0.4008	129.4321	1.1092	95.1307
37	0.0246	0.0205	-0.0129	0.0414	-0.0021	0.0039	-0.0489	0.0714	0.09	-0.0079	-0.0805	-0.0594	-0.0734	0.0618	-0	0.4933	-0.2236	-0.0219	0.5431	0.1168	0.5122	120.8569	64.496	56.4549
38	0.0161	-0.0045	0.0109	0.0388	-0.0209	0.0095	0.0118	-0.0449	0.0818	0.0142	-0.0464	-0.0721	0.0255	0.087	0	0.2805	-0.0345	-0.0428	0.4339	0.0955	0.3287	108.7866	-2.246	71.4143
39	0.005	-0.0017	-0.0015	0.0042	0.0016	0.0004	-0.0055	-0.0076	-0.0196	0.0123	-0.0249	-0.0504	0.0124	-0.013	-0	0.5133	0.2325	-0.0589	0.7876	0.1167	0.8066	149.8928	-61.431	52.8835
40	0.008	-0.0013	-0.0003	0.0146	-0.0003	0.0054	0.009	-0.0049	-0.0267	0.0174	-0.041	-0.0688	0.0086	-0.0136	0	0.3211	0.0023	-0.0655	0.4842	0.1122	0.424	102.2362	-23.5541	76.2601
41	0.0114	0.0002	-0.0044	0.0113	-0.0097	0.01	0.0072	0.0037	0.0333	-0.0419	0.0395	0.0554	0.0124	-0.0068	-0	0.4224	-0.064	-0.0608	0.6595	-0.0884	0.3032	1.8358	-16.8696	7.721
42	0.0075	-0.0056	0.0004	0.0039	-0.0008	0.002	-0.004	0.0219	-0.0163	-0.0094	0.0024	-0.0197	-0.0149	-0.0026	-0	0.566	0.0848	0.0907	0.6341	0.0538	0.5042	87.3611	-4.8398	-22.3928
43	0.0251	0.0189	0.0108	0.0162	-0.01	0.0098	-0.0135	0.0564	0.0542	-0.1178	-0.0017	-0.0499	-0.0048	-0.0054	0.001	0.337	-0.0598	-0.0199	0.2459	0.1451	0.3421	73.5797	61.5354	32.1687
44	0.0121	-0.0015	0.0011	0.0026	-0.0113	0.0229	0.0035	0.0372	-0.0171	-0.0253	0.0092	-0.0697	-0.0073	0.0266	-0	0.2956	0.0491	0.1624	0.4575	0.0683	0.4618	125.5207	-13.3724	1.7661
45	0.0826	-0.0104	0.0523	0.0203	0.0072	0.058	0.0065	0.017	0.0163	0.0072	-0.0098	-0.0161	0.0078	-0.0201	0	0.9934	-0.0586	0.0321	0.9699	0.0525	0.9777	139.0114	-6.7601	116.1683
46	0.014	0.0105	-0.0355	0.086	0.0196	0.0354	-0.0022	-0.0062	-0.0828	0.1082	-0.0983	0.0421	0.0659	0.0105	-0.0067	0.721	0.1087	0.024	0.6534	0.1545	0.3094	49.2377	40.9749	-47.8154
47	0.1006	0.025	0.0058	0.113	-0.0137	0.1649	-0.0456	0.0322	0.0227	0.0048	0.0218	0.0389	-0.013	-0.1105	-0.0033	0.2198	-0.0002	-0.0377	0.2182	-0.0165	0.2745	198.5373	-2.6944	176.2428
48	-0.043	0.0027	0.0676	0.0263	-0.0297	0.1007	-0.0498	0.0843	0.0195	0.0007	0.1167	-0.3377	-0.0838	0.1096	0.0006	0.4124	-0.1408	-0.1452	0.1818	-0.1132	0.569	266.5653	57.5968	187.2309
49	0.1804	-0.0266	0.1169	0.1836	-0.0504	0.1836	-0.012	0.0814	-0.019	-0.0639	0.0336	0.1687	0.0417	-0.264	0.0167	0.2067	-0.0217	-0.0828	0.6423	0.0159	0.4632	144.4355	-0.409	153.5668
50	0.0217	-0.0332	0.0089	0.0467	-0.0197	0.0224	-0.0386	-0.0055	0.0943	0.0813	0.0117	0.0315	-0.1153	-0.1808	-0.0139	0.2376	0.117	0.0582	0.485	-0.0692	0.3667	153.3356	7.2194	220.215
51	0.0771	0.052	-0.0014	0.0983	0.0037	0.0814	-0.0451	0.0573	-0.0726	0.0173	0.0108	-0.0061	0.1112	-0.0426	-0.0009	0.3863	-0.0478	0.0738	0.0587	-0.0515	0.3296	227.1212	-66.4215	175.308
52	0.0724	-0.0168	0.0467	0.1347	0.0636	0.1214	-0.0745	0.1119	0.0345	-0.0852	0.0261	0.0637	-0.0468	-0.034	-0.0667	0.2781	-0.019	-0.1298	0.3455	0.1159	0.2212	199.0552	26.5814	121.326
53	0.0237	0.0923	0.053	0.1412	0.0229	0.0665	-0.121	0.0905	0.0427	-0.1111	0.107	-0.1537	-0.1921	0.1245	-0.0009	0.6505	-0.2094	-0.2512	0.0658	0.0241	0.5176	256.8461	85.4386	188.372
54	0.0277	0.0012	0.0064	0.0332	0.0185	0.0166	-0.0573	0.1778	-0.0247	-0.0946	0.0165	0.0222	-0.052	0.0411	-0	0.3612	-0.1175	0.0672	0.499	-0.0767	0.2504	254.0414	-16.7442	101.6576
55	0.0133	-0.0046	-0.0005	0.0361	0.0113	0.0484	-0.0271	0.0884	0.0707	-0.0411	0.0554	-0.0494	-0.0485	0.0355	0.0034	0.275	-0.1268	-0.022	0.2164	0.0592	0.2585	257.5072	25.3154	134.0546
56	0.1103	0.0002	0.0228	0.049	-0.0046	0.0116	-0.0088	-0.0246	0.1271	-0.0002	-0.0242	0.1634	-0.0918	-0.1695	-0.0031	0.5068	0.4082	0.0328	0.8301	-0.0513	0.8861	119.1034	49.2044	193.2044
57	0.0201	0.0268	0.0331	0.0144	0.0041	0.0494	0.041	0.0893	-0.0978	0.0239	0.0406	-0.0537	0.0306	0.0636	0.0007	0.3419	0.0869	-0.0574	0.2504	-0.0271	0.4815	279.044	-44.6318	184.6973
58	0.0412	0.0035	0.0179	0.0327	0.0071	0.0359	0.0676	-0.0675	-0.0717	0.1982	0.0497	-0.3168	-0.0182	0.0577	0.1845	0.4151	0.0511	-0.533	0.2478	-0.1279	0.6982	265.9795	16.8767	198.2809
59	0.016	0.0185	-0.0137	0.038	-0.0337	0.1552	0.0619	-0.0437	0.0891	0.0879	0.0135	-0.1113	0.007	-0.086	0.0087	0.6605	-0.058	-0.1919	0.2439	-0.0362	0.4915	257.8554	69.8636	225.0048
60	0.0431	0.0054	0.0062	0.0349	0.0274	0.0078	0.0063	0.0858	0.04	0.0119	0.03	0.0677	-0.079	-0.0865	0.0743	0.398	0.2949	-0.2933	0.4443	0.1094	0.5179	166.6448	60.3259	146.9858
61	0.0509	-0.0333	-0.0303	0.0142	0.0042	0.0354	-0.1132	0.0823	0.0265	-0.0641	0.0583	-0.0141	0.1105	0.0227	-0	0.3291	-0.0551	0.0798	0.3868	-0.0684	0.4129	284.6031	-24.4732	136.7508
62	0.0218	0.021	0.0091	0.0027	0.034	0.0082	-0.0398	0.0026	0.0291	0.0471	0.0249	0.0901	-0.0835	-0.0885	-0.0083	0.2688	0.3158	-0.0787	0.4955	-0.0237	0.5271	150.1497	38.0954	176.4699
63	0.0475	-0.0301	0.0217	0.013	-0.0003	0.077	-0.0736	0.0829	-0.0617	0.0656	0.05	-0.0613	0.0854	0.0611	0.0014	0.1899	-0.0249	0.0184	0.1172	-0.0366	0.2082	255.58	-37.5459	150.8406
64	0.0711	-0.0027	0.0873	0.214	-0.0102	0.1277	-0.069	0.1621	0.0044	-0.2281	-0.0199	-0.1294	0.0256	0.0403	-0.036	0.3346	-0.1406	0.013	0.3183	-0.0681	0.3357	197.6739	-39.9127	137.5616
65	0.0419	0.0294	-0.0308	0.0519	0.0058	0.0162	-0.0658	0.1056	0.0703	-0.0224	-0.024	0.0589	-0.0878	0.001	-0.0399	0.4567	-0.0044	-0.145	0.2629	0.1252	0.3395	211.0092	50.0292	133.4039
66	0.0558	0.0427	0.0456	0.0044	-0.0037	0.0286	0.0327	-0.0147	-0.056	0.0334	0.0305	0.1378	0.0242	-0.0392	0.0052	0.378	-0.2163	0.0832	0.5498	0.0687	0.4792	148.346	-56.4794	222.4576
67	0.0723	-0.0114	-0.0668	0.0251	-0.006	0.0611	-0.0707	0.0758	0.0189	0.0546	0.0626	-0.0214	0.0126	0.0645	-0	0.239	-0.0624	-0.0598	0.2184	-0.0263	0.351	278.975	4.1542	158.5389
68	0.0436	-0.0514	0.0088	0.0543	-0.0337	0.0364	-0.0281	-0.0208	0.0312	0.0088	0.0299	-0.0124	-0.0993	-0.0168	0.0043	0.3501	-0.0881	-0.0938	0.1575	-0.0119	0.3901	250.3337	48.6724	165.5717
69	0.0077	-0.0021	0.0048	0.0083	0.0031	0.0025	0.0291	0.0343	-0.0857	-0.0313	-0.0062	0.0461	0.0024	-0.0203	0	0.7385	-0.4585	-0.09	0.754	0.0559	0.9546	144.7471	-85.1999	188.6674
70	0.005	-0.0001	0.0133	0.0227	-0.0021	0.0134	-0.0502	-0.0741	-0.037	0.0742	-0.018	-0.0366	0.0523	0.0601	-0	0.4931	-0.0284	0.0037	0.2034	0.049	0.322	222.9435	-51.6076	216.7396
71	-0.004	-0.012	0.0046	0.0222	0.0252	0.029	-0.0598	0.051	0.0705	-0.0147	0.019	0.0087	0.0631	-0.0377	0	0.5803	-0.0378	0.0589	0.2011	0.0116	0.4537	210.1488	-85.8196	213.1661
72	0.0418	0.0059	0.0208	0.022	-0.0312	0.0268	0.0201	-0.0172	0.0037	-0.0307	0.04	0.0909	-0.0079	-0.1613	0	0.2898	-0.0977	-0.069	0.7446	-0.0218	0.6084	126.3032	-16.102	165.2225
73	0.0063	0.0042	0.0025	0.0272	0.012	0.0137	-0.0682	-0.0189	-0.0472	-0.0196	0.0108	0.0318	0.0369	-0.1172	-0.0669	0.2891	-0.0938	-0.2177	0.5845	0.0124	0.3722	153.3067	-4.045	114.9379
74	0.0183	0.0082	-0.005	0.0015	-0.0013	0.0085	-0.0782	-0.0502	-0.0105	-0.0385	-0.0335	0.0831	0.0172	-0.0367	-0.0001	0.4152	-0.0953	0.2061	1.3051	0.0366	0.9356	100.5304	-17.9059	228.0236
75	0.0453	-0.0124	0.0338	0.0318	-0.0116	0.0466	-0.0414	-0.0091	0.0955	-0.0357	0.0399	0.0575	-0.0896	-0.065	0.0052	0.3884	0.109	-0.2354	0.2826	0.1314	0.4168	194.4343	55.8169	149.406
76	0.0136	0.0089	0.0255	0.012	0.0156	0.0477	-0.0481	0.0202	0.0303	-0.0068	0.0022	-0.0206	-0.0714	-0.0451	0	0.4912	-0.0574	-0.1721	0.2693	0.0484	0.3654	228.1427	53.2071	157.2159
77	0.0086	-0.0016	-0.008	0.004	-0.0021	0.0096	-0.026	-0.0066	0.0068	0.0137	-0.0472	0.0118	0.0001	-0.0296	-0	0.5583	0.005	0.1976	1.0212	-0.0105	0.6729	124.1549	-18.2481	251.037
78	0.0175	-0.009	0.0092	0.021	-0.0072	0.0071	0.0602	0.02	-0.0281	-0.0283	0.0087	0.006	-0.05	-0.033	0	0.5047	-0.1779	-0.0093	0.3241	-0.0431	0.435	182.8797	-71.3804	166.4758
79	0.031	-0.0092	0.0431	0.0154	0.0258	0.0704	-0.0894	0.1408	-0.0822	-0.0604	0.0012	-0.075	0.0226	-0.0219	-0.0152	0.2667	-0.0623	-0.0651	0.353	0.0236	0.1583	211.7343	0.0609	121.922
80	0.0121	-0.0099	-0.0165	0.0195	0.003	0.019	0.0104	0.0615	0.0043	-0.0605	0.0421	0.0016	0.005	-0.0001	0	0.3255	-0.0927	0.0248	0.318	-0.0423	0.227	245.8976	-19.2662	126.7001
81	0.0184	0.0078	-0.0121	0.0204	-0.0219	0.0067	-0.0271	-0.0109	-0.0352	-0.0261	0.0841	0.0287	-0.0195	-0.1304	0	0.3006	-0.0586	0.0508	0.7383	0.0107	0.5506	128.6555	-20.9041	211.5954
82	0.0763	-0.0105	0.0618	0.0163	-0.0147	0.0567	-0.0413	0.0406	0.087	-0.0179	0.0443	0.0228	-0.0238	-0.2546	-0.0015	0.3358	-0.0088	-0.1813	0.7295	0.0624	0.5814	129.3551	10.2805	145.3002
83	0.0183	-0.0004	-0.0066	0.0362	0.0147	-0.0001	-0.0471	-0.0695	0.0788	0.034	-0.0395	0.0619	-0.051	0.0106	-0	0.5632	0.2757	-0.1584	0.5404	-0.0208	0.7207	154.8232	69.1403	183.5536
84	0.0308	0.0122	0.0034	0.0364	0.0259	0.0154	0.0128	0.0738	0.0655	0.0426	-0.0195	0.0685	0.0259	-0.1143	0	0.3055	0.0242	-0.0851	0.5317	0.014	0.4851	154.3781	2.8892	163.1583
85	0.0143	0.0055	-0.0065	0.0057	-0.0005	0.0028	-0.0017	0.001	0.0145	0.0241	-0.0106	0.0142	0.0146	-0.0191	0	0.5096	-0.3127	0.0553	0.9518	-0.0301	1.0051	111.5682	-52.4484	188.076
86	0.0086	0.0035	0.003	0.0112	0.0029	0.0056	0.002	-0.05	-0.0124	-0.0798	-0.05	0.0556	0.0116	-0.0018	-0	0.3288	-0.0361	-0.0351	0.5884	0.0233	0.4757	156.9041	-18.704	155.9538
87	0.0109	0.0076	-0.0018	0.009	0.0033	0.0108	0.0658	0.0026	0.0448	0.0549	0.0416	-0.0415	-0.0209	0.0196	-0	0.4146	0.0687	-0.0967	0.2411	0.0224	0.4143	270.197	-28.3413	199.1143
88	0.0005	-0.0032	0.0031	0.0011	-0.0004	0.0038	0.0457	0.0109	-0.0378	0.0244	-0.0034	0.0271	-0.0134	0.0074	-0	0.425	0.0164	0.0134	0.3606	0.0185	0.2725	194.5597	-9.2772	249.0203
89	0.0091	0.0156	-0.0021	0.0121	0.0328	0.0636	-0.0053	-0.0161	0.1206	0.1133	-0.031	-0.0067	-0.0249	-0.0647	-0.0237	0.4439	0.1308	-0.1267	0.1998	-0.0438	0.4567	205.7106	59.4819	195.4171
90	0.0073	-0.0083	0.012	-0.0008	0.002	0.0171	0.0089	-0.0195	-0.0494	0.0461	0.0134	0.1154	0.0124	-0.0845	0	0.3143	-0.0156	0.1245	0.503	-0.0039	0.4473	152.892	-11.6327	234.1965
91	-0.0104	-0.0326	-0.0239	0.0552	0.045	0.0309	-0.103	0.0431	-0.0147	-0.1457	0.1067	0.1122	-0.0391	0.0324	-0.0001	0.628	-0.1271	-0.0715	0.4912	0.0109	0.5853	245.9783	39.625	259.7229
92	0.1664	-0.1737	-0.0288	0.2563	-0.0818	0.155	0.0864	0.365	-0.0329	-0.2339	-0.142	-0.1255	-0.0145	-0.0606	0.4471	0.4752	0.1782	0.076	0.6767	0.0526	0.3037	82.416	-19.0549	-52.0896
93	0.0062	-0.0011	-0.0076	0.0174	-0.0127	0.021	0.0095	-0.0045	0.0933	-0.0176	-0.0189	-0.0973	-0.0982	-0.0346	0	1.0713	0.2695	0.4536	1.163	0.4315	0.8686	126.5873	8.7898	-92.6942
94	0.0107	0.0106	-0.0073	0.0106	-0.0099	0.0066	-0.0382	0.0127	0.063	-0.019	-0.0116	-0.112	0.0047	0.0601	0	0.7448	-0.2716	0.1636	0.8473	0.1051	1.3156	145.5144	28.4135	-25.0241
95	0.0274	-0.03	0.0164	0.0272	-0.0151	0.0471	-0.0499	0.1122	0.0036	-0.137	-0.0752	-0.042	0.0232	0.0406	-0	1.0069	0.2615	0.1444	1.1109	0.1563	0.4533	91.956	-9.0925	-88.405
96	0.014	-0.0152	-0.0016	0.0085	-0.0028	0.0359	-0.0422	0.051	-0.0543	-0.0542	-0.0607	-0.0754	0.0354	-0.1068	-0.0001	0.982	0.3128	0.0666	1.1336	0.2004	0.5282	61.0386	29.959	-91.6338
97	0.0223	0.0093	-0.0218	0.0307	0.0035	0.0311	-0.0538	0.0812	-0.0456	-0.2119	-0.0008	-0.0608	0.0639	0.0221	0	0.8935	0.106	0.0621	0.9378	-0.3304	0.677	82.9291	-80.8455	-68.2692
98	0.0235	0.019	-0.0022	0.0183	0.0142	0.0138	0.0595	0.0006	0.0922	0.0008	0.0296	-0.0163	-0.0697	0.0532	0.0001	0.9633	0.03	0.047	0.842	0.4157	1.1573	102.1517	77.9982	-61.6369
99	0.0618	0.0699	0.0238	0.0916	-0.0059	0.0426	0.1524	-0.0647	-0.0802	0.0037	-0.1057	0.1016	0.1253	-0.0436	0.0023	0.7119	-0.1762	0.0867	0.6335	-0.3428	0.8355	52.9899	-97.8247	-22.2455
100	0.0131	0.0184	0.0153	0.0232	0.0238	0.0216	0.0139	0.0607	0.0036	0.0368	-0.0584	-0.0616	0.0265	-0.0589	0.0058	0.7202	0.3413	0.4327	0.9899	0.0677	0.8283	150.385	-47.6268	-75.6575
101	0.0072	-0.006	-0.0153	0.0015	-0.0089	0.0315	0.0271	0.0299	-0.1683	0.022	-0.0611	-0.0664	0.0448	0.0708	-0.0071	0.463	0.1428	0.1054	0.3614	-0.1317	0.6194	98.9364	-88.7858	-16.441
102	0.0168	-0.0141	-0.0118	0.0209	0.0006	0.0262	-0.0698	0.0618	-0.0821	-0.197	-0.0098	0.096	-0.0078	-0.0504	-0	1.1485	0.1948	-0.0455	1.2579	-0.1762	0.4967	62.9295	-41.0376	-88.3042
103	0.0014	0.0007	-0.0048	0.0014	-0.0022	0.0104	-0.0407	0.0136	-0.033	0.0468	-0.0297	0.0197	-0.0291	-0.0293	-0.0001	0.8759	0.0965	-0.0069	1.1049	0.1195	0.5311	27.3494	33.8717	-83.5888
104	0.0263	-0.0221	-0.0311	0.0077	0.0134	0.0377	0.02	0.0256	-0.0124	-0.0179	-0.0594	-0.068	-0.005	0.0404	0	0.5464	0.1036	0.1131	0.6901	-0.1056	0.6138	112.1673	-66.7493	-35.5536
105	0.0031	0.0037	0.0062	0.0027	0.0027	0.004	-0.0331	0.0174	-0.0228	-0.021	0.0087	-0.009	0.0146	0.004	0	1.4035	-0.0179	-0.1142	1.577	-0.1218	1.4315	-5.5098	26.2159	-81.1472
106	0.0173	-0.0132	-0.0085	0.0126	0.0051	0.0038	0.0677	0.0997	-0.0094	-0.0543	-0.0585	-0.0701	0.0872	0.0439	0	0.4126	0.0985	0.1748	0.6767	0.1798	0.7527	140.2692	-29.6607	-39.6191
107	0.006	-0.009	0.0053	0.0101	-0.01	0.0046	0.007	-0.0163	-0.0187	-0.0131	0.0144	-0.034	-0.0165	0.0034	-0	0.8917	-0.0946	0.0679	0.4543	0.1906	0.9711	122.2354	40.2957	-26.538
108	0.0125	-0.0097	-0.004	0.0079	0.0047	0.0154	0.0188	0.0221	0.026	-0.0079	-0.0586	0.0198	0.0358	0.005	-0	0.9485	0.2465	0.1498	0.8284	0.2459	0.5118	29.0762	71.0164	-42.9945
109	0.0312	-0.0055	0.0037	0.0221	0.0186	0.0118	0.0457	0.0188	0.0759	0.0014	0.0834	-0.0674	-0.0017	-0.0028	0.0011	0.9438	0.2589	0.2147	0.9764	0.5649	1.0055	88.2001	78.0928	-85.7692
110	0.018	0.0104	-0.0269	0.0109	-0.0426	0.0407	-0.0212	-0.0043	0.0321	-0.0483	0.0416	0.0258	-0.1656	-0.1501	0.0001	0.8737	0.2626	0.2723	0.8869	0.4121	0.7399	115.0615	30.9808	-95.7239
111	-0.0019	-0.0004	-0.005	0.0061	0.0034	0.0038	0.029	-0.0163	0.1285	-0.0031	0.0355	-0.0824	-0.0666	-0.0366	0	1.0261	0.1289	0.1631	0.6481	0.282	0.6513	56.645	91.1366	-41.1832
112	0.0102	-0.0214	-0.0007	0.0443	0.01	0.0082	0.0712	0.0109	0.0464	0.0063	-0.0088	-0.031	0.0498	-0.0288	0.0053	1.1217	0.2864	0.285	0.7939	0.5325	0.834	64.4695	96.1721	-73.951
113	0.0386	0.0075	0.0059	0.02	0.0107	0.0051	-0.0318	-0.085	-0.0662	0.0427	-0.0834	0.0786	0.0181	0.015	-0.0028	0.8923	0.2934	0.0195	0.9367	0.3065	0.4326	59.6135	56.5029	-99.7447
114	0.0061	-0.0002	-0.0063	-0.0011	0.0078	0.0214	-0.0161	0.0346	-0.0007	-0.0378	-0.0221	-0.0068	0.0028	-0.0212	-0	1.1142	0.1769	0.3747	1.1702	0.0985	0.7744	117.9649	-11.5208	-94.3697
115	0.0177	-0.0063	0.0022	0.0259	-0.0074	0.0166	0.021	0.0977	0.007	-0.0693	-0.0904	-0.0801	0.0604	-0.0495	-0.0042	0.7381	0.2407	0.3431	0.9035	0.0446	0.5039	114.9297	-32.8216	-73.5514
116	0.0112	0.0274	-0.0126	0.047	-0.0287	0.0238	-0.0019	0.0043	-0.0158	-0.1422	0.0409	0.0315	0.008	-0.0272	0	0.5326	0.1345	0.0352	0.755	0.0076	0.4544	64.3864	-20.8432	-45.3138
117	0.0376	0.0117	0.0196	0.0213	0.0075	0.0125	0.0535	0.0962	-0.0527	-0.0723	0.0441	0.1179	0.0203	-0.0152	0.0001	0.8112	0.0202	-0.1433	0.9243	-0.339	0.7156	44.6816	-76.5218	-68.2651
118	-0.0014	0.0006	0.0008	0.0035	-0.0021	-0.0002	-0.0008	-0.0252	0.0085	-0.0095	-0.0208	-0.0057	0.0008	0.0016	0	1.3039	0.0085	-0.0198	1.0538	0.169	1.3104	90.751	102.9211	-50.353
119	0.0107	-0.0031	-0.0029	0.0054	-0.0099	0.018	0.0041	0.0169	0.0267	-0.0225	0.0134	-0.0433	-0.007	-0.0384	-0	0.8135	-0.0472	0.1769	0.7745	0.2513	0.9067	118.424	23.604	-43.7375
120	0.0184	-0.004	-0.0231	-0.0033	0.003	0.0193	-0.0208	0.0485	-0.0103	0.0165	-0.018	-0.0797	0.0002	-0.0356	0	0.6816	0.0078	0.3135	0.9894	0.1692	0.9042	152.6726	-16.9449	-58.191
121	0.0027	-0.003	0.0011	0.0018	-0.002	-0.0003	0.0221	-0.011	0.016	0.0127	0.0198	-0.0126	0.0076	0.0086	-0	1.0195	0.1678	-0.0078	1.1402	0.2829	1.2871	118.336	57.755	-58.5051
122	0.0056	-0.0062	0.0109	0.0024	-0.0087	0.0184	-0.0008	0.0255	-0.0124	-0.0809	-0.0325	-0.0352	0.0235	0.0031	0	0.828	0.2307	0.0724	0.9019	0.2857	0.5052	49.0004	48.4651	-73.379
123	0.0278	-0.0245	0.0159	0.0116	-0.0114	0.0084	-0.0181	0.0253	-0.0167	-0.0791	0.0546	0.0383	0.0318	-0.0183	-0	0.6354	0.1722	-0.032	0.8245	-0.0078	0.5448	49.7908	-12.1171	-73.9985
124	0.0039	-0.0001	-0.0005	-0.0039	-0.0015	-0.0021	-0.0419	0.0074	0.0177	0.015	-0.0238	0.0531	-0.0147	0.0097	-0	0.9201	0.0599	-0.0415	1.2065	0.0046	1.0028	2.5887	43.6869	-60.6159
125	0.0099	-0.0039	-0.0091	0.0128	-0.0178	0.0384	-0.0216	0.0409	0.0535	-0.0933	0.0533	-0.1311	-0.0234	0.0635	0.0001	1.1258	0.336	0.3664	1.3014	0.5655	1.0275	106.7741	58.4895	-117.8562
126	0.0059	0.0116	0.0025	0.0163	0.0023	0.0142	0.0043	-0.0407	0.1154	0.1199	-0.0828	0.091	-0.0362	0.0483	0	1.3307	-0.2047	0.4379	0.8186	-0.1804	0.6932	171.283	1.689	326.8551
127	0.0428	-0.028	-0.0496	0.0489	0.041	0.0369	-0.0181	-0.0632	0.137	0.0257	-0.1138	0.1494	-0.0628	-0.1365	-0	0.7811	0.2564	0.19	0.8469	-0.1532	0.7824	139.2468	25.6679	264.9158
128	0.063	-0.0089	0.0122	0.0371	0.0167	0.0431	0.0751	-0.1251	0.0251	0.1118	-0.0704	-0.0374	-0.0075	-0.029	0	1.1791	-0.185	0.0219	0.6738	0.0088	0.4465	203.3596	-16.9646	314.1507
129	0.0193	-0.0269	0.0213	0.0408	-0.0428	0.0263	-0.1673	-0.1048	-0.0649	0.065	-0.0674	-0.0627	0.0673	0.1126	-0.0001	0.9662	0.0603	0.0451	0.8789	-0.0345	0.8492	234.7846	23.2476	315.2678
130	0.0401	0.0124	-0.0278	0.0259	0.0218	0.0539	-0.0367	-0.1045	-0.1478	0.2625	-0.0226	0.1409	0.1052	-0.0434	-0.0047	1.1875	-0.0729	0.0366	0.4416	0.1929	0.6152	203.8354	-87.9184	289.1612
131	0.0425	-0.035	0.0049	0.0172	-0.0029	0.0023	0.0366	0.0715	0.1854	0.0117	0.0756	0.0337	-0.0011	-0.1489	0	0.6867	-0.1652	0.0624	0.8336	0.1668	1.3264	190.411	70.3225	289.224
132	0.0538	-0.0112	0.0169	0.139	0.0901	0.0833	0.0878	0.0844	-0.1566	0.1056	-0.0371	-0.0728	0.0947	-0.113	0.0005	0.7681	0.101	-0.0149	0.3403	0.0108	0.7276	223.6163	-103.5496	235.8054
133	0.011	0.0116	0.0076	0.0078	-0.001	0.0233	0.0318	-0.0481	-0.0857	0.0291	-0.0278	0.0316	-0.0149	0.0066	-0	0.9783	-0.2824	0.3133	0.9943	0.0998	0.7163	141.7401	-52.8502	312.926
134	0.0358	-0.0295	0.0067	0.0224	-0.0159	0.0355	0.0388	0.0228	-0.1568	0.0173	-0.012	0.1264	0.1305	-0.1027	0	0.6399	-0.1809	-0.0263	0.3353	-0.0418	0.7453	176.8859	-92.2184	241.7191
135	0.0051	-0.0028	0.0062	0.0227	-0.0105	0.0247	-0.0278	-0.0941	-0.038	0.2456	0.06	-0.0501	0.03	0.0273	-0	1.2718	-0.0152	-0.2276	0.7365	0.0907	0.5502	229.8867	-50.1507	306.2507
136	-0.0068	-0.0009	0.0152	0.0263	-0.0246	0.0197	0.0092	-0.0125	-0.0918	0.0722	0.0358	0.1622	-0.0113	-0.0047	0	0.7134	-0.1992	0.1477	0.5057	0.0335	0.7178	170.0882	-71.4261	264.1025
137	0.0018	-0.0034	0.0006	0	-0.0005	-0.001	0.0257	-0.0079	0.0055	0.0575	0.0194	-0.0183	0.0075	0.0174	0	1.8841	0.159	0.0761	2.049	0.092	2.1183	299.8162	19.8356	296.5946
138	0.0146	0.001	-0.0158	0.0288	0.0011	0.0126	0.0193	-0.0857	-0.0497	0.0196	-0.0514	0.0988	-0.0165	-0.0656	-0	0.6439	-0.1517	0.2687	0.7575	-0.0187	0.6687	144.7637	-33.1998	275.9953
139	-0.003	-0.0067	0.007	0.0083	0.0087	0.028	-0.0854	0.027	0.0703	-0.0235	-0.0057	-0.0301	0.0126	0.0524	-0	0.8424	0.1327	0.0709	0.5362	-0.0982	0.6607	162.3502	37.4882	262.0731
140	0.0094	0.0058	-0.0048	0.0003	0.0054	0.0246	-0.0862	0.0352	0.1298	-0.0622	-0.0805	-0.0409	0.0625	0.0256	-0	0.95	-0.2431	0.2256	0.9587	-0.0777	0.9764	259.8258	60.0838	259.722
141	0.0296	0.0096	-0.0152	0.0334	-0.0288	0.019	-0.0068	-0.0217	0.1506	0.0035	0.0703	0.0279	0.0496	-0.0532	-0	0.8581	-0.0555	0.2035	0.585	-0.2643	1.0625	207.8405	71.3574	311.0711
142	0.0163	-0.009	-0.0272	0.0158	0.0305	0.0341	-0.0847	0.0215	0.0091	0.0872	-0.0366	0.0567	-0.1031	0.0409	-0	1.0522	-0.0812	0.2347	0.8822	-0.2595	0.7638	183.5893	25.793	327.941
143	0.0054	0.0144	-0.0165	0.0203	-0.0206	0.0243	-0.029	0.1175	0.1119	-0.044	0.0548	0	0.0182	-0.0367	0	0.9203	-0.378	0.0047	0.9273	0.2106	1.1478	232.5517	81.2433	261.5084
144	0.0075	0.0105	-0.0144	0.0138	-0.0056	0.0103	0.0017	-0.0141	0.1515	0.0323	0.0676	-0.0088	-0.0243	0.0193	0	0.9128	-0.1826	0.0437	0.6372	-0.1812	1.2438	229.7469	88.1652	294.8951
145	0.0056	0.0007	0.0014	0.0042	-0.0028	0.0048	-0.0182	-0.0179	0.0129	0.011	-0.0327	-0.0163	-0.0184	0.001	-0	1.0728	0.249	0.0759	1.1354	0.116	1.1629	266.8792	27.4315	302.8828
146	0.0014	-0.0134	0.0078	0.024	-0.0129	0.0041	-0.0806	-0.0228	-0.0781	-0.0761	-0.0573	-0.0384	0.0702	0.0077	0	0.8984	-0.0897	-0.0887	0.7642	-0.166	0.6949	238.4526	50.5723	322.472
147	0.007	-0.0055	-0.0041	0.0038	0.0041	0.0111	0.0749	-0.0589	0.0212	0.0598	-0.0221	0.0116	0.0026	-0.0338	0	1.1483	-0.1212	0.2576	0.9212	-0.0251	0.4787	178.9198	-18.799	325.439
148	0.0106	0.0116	0.0007	0.0104	-0.0101	0.0196	-0.021	-0.0694	-0.0436	0.1126	-0.0296	-0.0095	0.0326	-0.0061	0	0.9732	-0.1273	0.2617	0.6696	0.0427	0.4514	176.5292	-38.8515	303.4877
149	0.0136	-0.0076	0.0064	0.0406	0.0251	0.0309	-0.0326	-0.0151	0.0339	0.1897	0.0071	-0.0514	-0.0134	0.0009	-0	0.614	-0.0814	-0.0158	0.4448	-0.0165	0.4451	220.5073	-27.4195	265.6986
150	0.0289	-0.0334	-0.0011	0.0201	0.0048	0.0063	0.0103	0.0027	-0.1299	0.2113	-0.0065	-0.1299	0.043	0.0772	0	0.9386	0.1392	-0.2683	0.517	0.1238	0.6939	241.3676	-85.3361	281.284
151	0.0048	0.0041	-0	0.0042	-0.0009	-0.0008	0.0086	0.0181	-0.0324	-0.0118	-0.0108	0.0127	-0.0334	-0.0283	0	0.9846	-0.0442	0.1014	0.8933	0.2296	1.3537	200.4937	94.4201	275.1287
152	0.0021	-0.0042	0.0077	0.0057	0.0038	0.0261	0.0175	-0.0309	0.0339	0.0277	-0.01	0.0212	-0.0195	-0.0343	-0	0.9146	0.0555	0.183	0.6783	-0.1439	0.5265	169.4966	18.93	277.5593
153	0.0096	0.0003	-0.0133	0.0024	0.0029	0.015	-0.0066	-0.0281	-0.0095	0.0929	-0.0082	0.0618	-0.0063	0.0147	-0	0.7408	-0.0408	0.3808	0.8616	0.037	0.6715	137.0276	-20.9952	297.0972
154	0.0025	0.0005	-0.0029	-0.0005	-0	-0.0001	-0.0028	-0.0066	0.003	-0.0194	-0.0048	0.0338	-0.0188	-0.0188	0	0.9818	-0.2067	0.1724	1.1451	0.1377	1.2979	173.6389	50.3112	289.8421
155	0.0032	0.0011	0.0044	0.0069	0.0023	0.0043	0.007	0.0092	0.0054	0.0442	-0.0378	0.0348	0.026	0.0366	0	0.8624	-0.0019	0.0391	0.7605	-0.1948	0.8693	244.4464	40.5855	294.5468
156	0.0105	0.0142	0.0086	0.022	0.0181	0.0102	0.0179	0.0361	0.0405	0.0262	0.0204	-0.0288	-0.0028	0.0291	-0	0.7684	-0.1414	-0.1609	0.6559	0.0211	0.6421	241.3308	-20.6022	291.2169
157	0.0001	0.0006	-0.001	-0	-0.0021	0.0005	-0.0104	-0.0139	-0.0003	0.004	-0.0047	0.0121	0.0108	-0.009	0	1.6159	0.0659	0.0758	1.8145	0.1037	1.7421	296.6937	39.5992	280.2902
158	0.1826	-0.0142	0.0098	0.2515	0.0875	0.1119	0.0618	-0.0997	-0.0225	0.2065	-0.0541	0.0109	0.0606	-0.0114	-0.0006	0.6643	-0.1028	0.0536	0.4214	0.0318	0.3809	203.5695	-25.3755	276.0681

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 1	1	3 - 3396
2	X-RAY DIFFRACTION	2	chain 3	3	1 - 121
3	X-RAY DIFFRACTION	3	chain 4	4	1 - 158
4	X-RAY DIFFRACTION	4	chain j	j	2 - 253
5	X-RAY DIFFRACTION	5	chain k	k	2 - 387
6	X-RAY DIFFRACTION	6	chain l	l	2 - 362
7	X-RAY DIFFRACTION	7	chain m	m	2 - 297
8	X-RAY DIFFRACTION	8	chain n	n	2 - 176
9	X-RAY DIFFRACTION	9	chain o	o	23 - 244
10	X-RAY DIFFRACTION	10	chain p	p	24 - 256
11	X-RAY DIFFRACTION	11	chain q	q	1 - 191
12	X-RAY DIFFRACTION	12	chain r	r	2 - 221
13	X-RAY DIFFRACTION	13	chain s	s	6 - 174
14	X-RAY DIFFRACTION	14	chain t	t	2 - 194
15	X-RAY DIFFRACTION	15	chain u	u	3 - 138
16	X-RAY DIFFRACTION	16	chain v	v	2 - 204
17	X-RAY DIFFRACTION	17	chain w	w	3 - 199
18	X-RAY DIFFRACTION	18	chain x	x	2 - 184
19	X-RAY DIFFRACTION	19	chain y	y	2 - 186
20	X-RAY DIFFRACTION	20	chain z	z	2 - 189
21	X-RAY DIFFRACTION	21	chain 0	0	1 - 172
22	X-RAY DIFFRACTION	22	chain 2	2	2 - 160
23	X-RAY DIFFRACTION	23	chain 5	5	9 - 108
24	X-RAY DIFFRACTION	24	chain 6	6	2 - 137
25	X-RAY DIFFRACTION	25	chain 7	7	1 - 98
26	X-RAY DIFFRACTION	26	chain 8	8	22 - 142
27	X-RAY DIFFRACTION	27	chain 9	9	2 - 127
28	X-RAY DIFFRACTION	28	chain AA	AA	2 - 136
29	X-RAY DIFFRACTION	29	chain AB	AB	2 - 149
30	X-RAY DIFFRACTION	30	chain AC	AC	2 - 59
31	X-RAY DIFFRACTION	31	chain AD	AD	9 - 105
32	X-RAY DIFFRACTION	32	chain AE	AE	4 - 112
33	X-RAY DIFFRACTION	33	chain AF	AF	2 - 128
34	X-RAY DIFFRACTION	34	chain AG	AG	2 - 107
35	X-RAY DIFFRACTION	35	chain AH	AH	2 - 113
36	X-RAY DIFFRACTION	36	chain AI	AI	2 - 120
37	X-RAY DIFFRACTION	37	chain AJ	AJ	2 - 100
38	X-RAY DIFFRACTION	38	chain AK	AK	2 - 100
39	X-RAY DIFFRACTION	39	chain AL	AL	2 - 78
40	X-RAY DIFFRACTION	40	chain AM	AM	2 - 51
41	X-RAY DIFFRACTION	41	chain AN	AN	77 - 500
42	X-RAY DIFFRACTION	42	chain AO	AO	1 - 25
43	X-RAY DIFFRACTION	43	chain AP	AP	2 - 501
44	X-RAY DIFFRACTION	44	chain AQ	AQ	2 - 501
45	X-RAY DIFFRACTION	45	chain o1	o1	1 - 1515
46	X-RAY DIFFRACTION	46	chain i	i	9 - 176
47	X-RAY DIFFRACTION	47	chain AR	AR	3 - 3396
48	X-RAY DIFFRACTION	48	chain AS	AS	1 - 121
49	X-RAY DIFFRACTION	49	chain AT	AT	1 - 158
50	X-RAY DIFFRACTION	50	chain CD	CD	2 - 253
51	X-RAY DIFFRACTION	51	chain CE	CE	2 - 387
52	X-RAY DIFFRACTION	52	chain CF	CF	2 - 362
53	X-RAY DIFFRACTION	53	chain CG	CG	2 - 297
54	X-RAY DIFFRACTION	54	chain CH	CH	2 - 176
55	X-RAY DIFFRACTION	55	chain CI	CI	23 - 244
56	X-RAY DIFFRACTION	56	chain CJ	CJ	24 - 256
57	X-RAY DIFFRACTION	57	chain CK	CK	1 - 191
58	X-RAY DIFFRACTION	58	chain CL	CL	2 - 221
59	X-RAY DIFFRACTION	59	chain CM	CM	6 - 174
60	X-RAY DIFFRACTION	60	chain CN	CN	2 - 194
61	X-RAY DIFFRACTION	61	chain CO	CO	3 - 138
62	X-RAY DIFFRACTION	62	chain CP	CP	2 - 204
63	X-RAY DIFFRACTION	63	chain CQ	CQ	3 - 199
64	X-RAY DIFFRACTION	64	chain CR	CR	2 - 184
65	X-RAY DIFFRACTION	65	chain CS	CS	2 - 186
66	X-RAY DIFFRACTION	66	chain CT	CT	2 - 189
67	X-RAY DIFFRACTION	67	chain CU	CU	1 - 172
68	X-RAY DIFFRACTION	68	chain CV	CV	2 - 160
69	X-RAY DIFFRACTION	69	chain CW	CW	9 - 108
70	X-RAY DIFFRACTION	70	chain CX	CX	2 - 137
71	X-RAY DIFFRACTION	71	chain CY	CY	1 - 98
72	X-RAY DIFFRACTION	72	chain CZ	CZ	22 - 142
73	X-RAY DIFFRACTION	73	chain DA	DA	2 - 127
74	X-RAY DIFFRACTION	74	chain DB	DB	2 - 136
75	X-RAY DIFFRACTION	75	chain DC	DC	2 - 149
76	X-RAY DIFFRACTION	76	chain DD	DD	2 - 59
77	X-RAY DIFFRACTION	77	chain DE	DE	9 - 105
78	X-RAY DIFFRACTION	78	chain DF	DF	4 - 112
79	X-RAY DIFFRACTION	79	chain DG	DG	2 - 128
80	X-RAY DIFFRACTION	80	chain DH	DH	2 - 107
81	X-RAY DIFFRACTION	81	chain DI	DI	2 - 113
82	X-RAY DIFFRACTION	82	chain DJ	DJ	2 - 120
83	X-RAY DIFFRACTION	83	chain DK	DK	2 - 100
84	X-RAY DIFFRACTION	84	chain DL	DL	2 - 100
85	X-RAY DIFFRACTION	85	chain DM	DM	2 - 78
86	X-RAY DIFFRACTION	86	chain DN	DN	2 - 51
87	X-RAY DIFFRACTION	87	chain DO	DO	77 - 500
88	X-RAY DIFFRACTION	88	chain DP	DP	1 - 25
89	X-RAY DIFFRACTION	89	chain DQ	DQ	2 - 501
90	X-RAY DIFFRACTION	90	chain DR	DR	2 - 501
91	X-RAY DIFFRACTION	91	chain sM	sM	23 - 174
92	X-RAY DIFFRACTION	92	chain A	A	1 - 1798
93	X-RAY DIFFRACTION	93	chain B	B	2 - 207
94	X-RAY DIFFRACTION	94	chain C	C	20 - 233
95	X-RAY DIFFRACTION	95	chain D	D	34 - 250
96	X-RAY DIFFRACTION	96	chain E	E	3 - 225
97	X-RAY DIFFRACTION	97	chain F	F	2 - 261
98	X-RAY DIFFRACTION	98	chain G	G	20 - 225
99	X-RAY DIFFRACTION	99	chain H	H	1 - 226
100	X-RAY DIFFRACTION	100	chain I	I	4 - 187
101	X-RAY DIFFRACTION	101	chain J	J	2 - 200
102	X-RAY DIFFRACTION	102	chain K	K	2 - 186
103	X-RAY DIFFRACTION	103	chain L	L	1 - 96
104	X-RAY DIFFRACTION	104	chain M	M	2 - 156
105	X-RAY DIFFRACTION	105	chain N	N	20 - 143
106	X-RAY DIFFRACTION	106	chain O	O	2 - 151
107	X-RAY DIFFRACTION	107	chain P	P	11 - 137
108	X-RAY DIFFRACTION	108	chain Q	Q	8 - 131
109	X-RAY DIFFRACTION	109	chain R	R	3 - 143
110	X-RAY DIFFRACTION	110	chain S	S	2 - 126
111	X-RAY DIFFRACTION	111	chain T	T	2 - 146
112	X-RAY DIFFRACTION	112	chain U	U	2 - 144
113	X-RAY DIFFRACTION	113	chain V	V	15 - 121
114	X-RAY DIFFRACTION	114	chain W	W	1 - 87
115	X-RAY DIFFRACTION	115	chain X	X	2 - 130
116	X-RAY DIFFRACTION	116	chain Y	Y	2 - 145
117	X-RAY DIFFRACTION	117	chain Z	Z	2 - 135
118	X-RAY DIFFRACTION	118	chain a	a	36 - 105
119	X-RAY DIFFRACTION	119	chain b	b	2 - 500
120	X-RAY DIFFRACTION	120	chain c	c	2 - 101
121	X-RAY DIFFRACTION	121	chain d	d	5 - 67
122	X-RAY DIFFRACTION	122	chain e	e	4 - 99
123	X-RAY DIFFRACTION	123	chain f	f	2 - 61
124	X-RAY DIFFRACTION	124	chain g	g	82 - 501
125	X-RAY DIFFRACTION	125	chain h	h	2 - 319
126	X-RAY DIFFRACTION	126	chain s0	s0	2 - 207
127	X-RAY DIFFRACTION	127	chain s1	s1	20 - 235
128	X-RAY DIFFRACTION	128	chain s2	s2	34 - 250
129	X-RAY DIFFRACTION	129	chain s3	s3	3 - 225
130	X-RAY DIFFRACTION	130	chain s4	s4	2 - 261
131	X-RAY DIFFRACTION	131	chain s5	s5	20 - 225
132	X-RAY DIFFRACTION	132	chain s6	s6	1 - 218
133	X-RAY DIFFRACTION	133	chain s7	s7	2 - 187
134	X-RAY DIFFRACTION	134	chain s8	s8	2 - 200
135	X-RAY DIFFRACTION	135	chain s9	s9	2 - 186
136	X-RAY DIFFRACTION	136	chain c1	c1	2 - 147
137	X-RAY DIFFRACTION	137	chain c2	c2	20 - 143
138	X-RAY DIFFRACTION	138	chain c3	c3	2 - 151
139	X-RAY DIFFRACTION	139	chain c4	c4	10 - 137
140	X-RAY DIFFRACTION	140	chain c5	c5	4 - 138
141	X-RAY DIFFRACTION	141	chain c6	c6	2 - 143
142	X-RAY DIFFRACTION	142	chain c7	c7	2 - 122
143	X-RAY DIFFRACTION	143	chain c8	c8	2 - 146
144	X-RAY DIFFRACTION	144	chain c9	c9	2 - 144
145	X-RAY DIFFRACTION	145	chain c0	c0	1 - 98
146	X-RAY DIFFRACTION	146	chain d0	d0	12 - 121
147	X-RAY DIFFRACTION	147	chain d1	d1	1 - 87
148	X-RAY DIFFRACTION	148	chain d2	d2	2 - 130
149	X-RAY DIFFRACTION	149	chain d3	d3	2 - 145
150	X-RAY DIFFRACTION	150	chain d4	d4	2 - 135
151	X-RAY DIFFRACTION	151	chain d5	d5	37 - 105
152	X-RAY DIFFRACTION	152	chain d6	d6	2 - 500
153	X-RAY DIFFRACTION	153	chain d7	d7	2 - 1
154	X-RAY DIFFRACTION	154	chain d8	d8	5 - 67
155	X-RAY DIFFRACTION	155	chain d9	d9	4 - 99
156	X-RAY DIFFRACTION	156	chain e0	e0	2 - 63
157	X-RAY DIFFRACTION	157	chain e1	e1	102 - 501
158	X-RAY DIFFRACTION	158	chain sR	sR	1 - 1800