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Yorodumi- PDB-2m4z: Analysis of the structural and molecular basis of voltage-sensiti... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m4z | ||||||
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Title | Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV (-TRTX-Hh2a). | ||||||
Components | Mu-theraphotoxin-Hh2a | ||||||
Keywords | TOXIN / venom toxin | ||||||
Function / homology | Function and homology information host cell presynaptic membrane / ion channel inhibitor activity / sodium channel regulator activity / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Haplopelma schmidti (Chinese earth tiger) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Gibbs, A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2013 Title: Analysis of the Structural and Molecular Basis of Voltage-sensitive Sodium Channel Inhibition by the Spider Toxin Huwentoxin-IV ( mu-TRTX-Hh2a). Authors: Minassian, N.A. / Gibbs, A. / Shih, A.Y. / Liu, Y. / Neff, R.A. / Sutton, S.W. / Mirzadegan, T. / Connor, J. / Fellows, R. / Husovsky, M. / Nelson, S. / Hunter, M.J. / Flinspach, M. / Wickenden, A.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m4z.cif.gz | 215.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m4z.ent.gz | 179 KB | Display | PDB format |
PDBx/mmJSON format | 2m4z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m4z_validation.pdf.gz | 431.7 KB | Display | wwPDB validaton report |
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Full document | 2m4z_full_validation.pdf.gz | 584.2 KB | Display | |
Data in XML | 2m4z_validation.xml.gz | 23.8 KB | Display | |
Data in CIF | 2m4z_validation.cif.gz | 29 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m4/2m4z ftp://data.pdbj.org/pub/pdb/validation_reports/m4/2m4z | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4005.732 Da / Num. of mol.: 1 / Fragment: unp residues 53-87 / Mutation: A82W Source method: isolated from a genetically manipulated source Source: (gene. exp.) Haplopelma schmidti (Chinese earth tiger) Plasmid: pCDNA / Production host: Homo Sapiens (human) / References: UniProt: P83303 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: HWTX-IV(W30A) | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2 mM entity, 20 mM sodium phosphate, 150 uM sodium azide, 100 uM [U-2H] EDTA, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | pH: 6.7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 950 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 1000 / Conformers submitted total number: 20 |