+Open data
-Basic information
Entry | Database: PDB / ID: 2ii7 | ||||||
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Title | Anabaena sensory rhodopsin transducer | ||||||
Components | Anabaena sensory rhodopsin transducer protein | ||||||
Keywords | SIGNALING PROTEIN / rhodopsin / transducer | ||||||
Function / homology | TM1070-like / Anabaena sensory rhodopsin transducer / TM1070-like superfamily / Anabaena sensory rhodopsin transducer / Hypothetical Protein Tm1070; Chain: A / Sandwich / Mainly Beta / Alr3166 protein Function and homology information | ||||||
Biological species | Anabaena sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Vogeley, L. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Crystal structure of the anabaena sensory rhodopsin transducer. Authors: Vogeley, L. / Trivedi, V.D. / Sineshchekov, O.A. / Spudich, E.N. / Spudich, J.L. / Luecke, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ii7.cif.gz | 171.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ii7.ent.gz | 137.7 KB | Display | PDB format |
PDBx/mmJSON format | 2ii7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ii7_validation.pdf.gz | 491.9 KB | Display | wwPDB validaton report |
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Full document | 2ii7_full_validation.pdf.gz | 522.5 KB | Display | |
Data in XML | 2ii7_validation.xml.gz | 32.6 KB | Display | |
Data in CIF | 2ii7_validation.cif.gz | 44.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ii/2ii7 ftp://data.pdbj.org/pub/pdb/validation_reports/ii/2ii7 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 14704.335 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Anabaena sp. (bacteria) / Plasmid: pKJ / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q8YSC3 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.72 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.2 Details: 100 mM sodium acetate, 10% (w/v) PEG 4000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Detector: CCD / Date: Feb 16, 2005 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.6→47.1 Å / Num. obs: 32009 / % possible obs: 86.5 % / Redundancy: 4.41 % / Rmerge(I) obs: 0.103 / Χ2: 0.89 / Net I/σ(I): 7.6 / Scaling rejects: 1427 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→30 Å / σ(F): 0
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Solvent computation | Bsol: 25.663 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 61.157 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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Xplor file |
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