gap junction-mediated intercellular transport / water transport / water channel activity / structural constituent of eye lens / gap junction / lens development in camera-type eye / positive regulation of cell adhesion / visual perception / protein homotetramerization / calmodulin binding ...gap junction-mediated intercellular transport / water transport / water channel activity / structural constituent of eye lens / gap junction / lens development in camera-type eye / positive regulation of cell adhesion / visual perception / protein homotetramerization / calmodulin binding / apical plasma membrane / endoplasmic reticulum / plasma membrane Similarity search - Function
Glycerol uptake facilitator protein / Glycerol uptake facilitator protein. / Aquaporin transporter / Major intrinsic protein, conserved site / MIP family signature. / Major intrinsic protein / Major intrinsic protein / Aquaporin-like / Up-down Bundle / Mainly Alpha Similarity search - Domain/homology
Journal: Nature / Year: 2005 Title: Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Authors: Tamir Gonen / Yifan Cheng / Piotr Sliz / Yoko Hiroaki / Yoshinori Fujiyoshi / Stephen C Harrison / Thomas Walz / Abstract: Lens-specific aquaporin-0 (AQP0) functions as a specific water pore and forms the thin junctions between fibre cells. Here we describe a 1.9 A resolution structure of junctional AQP0, determined by ...Lens-specific aquaporin-0 (AQP0) functions as a specific water pore and forms the thin junctions between fibre cells. Here we describe a 1.9 A resolution structure of junctional AQP0, determined by electron crystallography of double-layered two-dimensional crystals. Comparison of junctional and non-junctional AQP0 structures shows that junction formation depends on a conformational switch in an extracellular loop, which may result from cleavage of the cytoplasmic amino and carboxy termini. In the centre of the water pathway, the closed pore in junctional AQP0 retains only three water molecules, which are too widely spaced to form hydrogen bonds with each other. Packing interactions between AQP0 tetramers in the crystalline array are mediated by lipid molecules, which assume preferred conformations. We were therefore able to build an atomic model for the lipid bilayer surrounding the AQP0 tetramers, and we describe lipid-protein interactions.
History
Deposition
Oct 3, 2005
Deposition site: RCSB / Processing site: RCSB
Revision 1.0
Dec 6, 2005
Provider: repository / Type: Initial release
Revision 1.1
May 1, 2008
Group: Version format compliance
Revision 1.2
Jul 13, 2011
Group: Derived calculations / Version format compliance
EXPERIMENT TYPE : ELECTRON DIFFRACTION DATE OF DATA COLLECTION : 01-DEC-03 TEMPERATURE (KELVIN) : ...EXPERIMENT TYPE : ELECTRON DIFFRACTION DATE OF DATA COLLECTION : 01-DEC-03 TEMPERATURE (KELVIN) : 300.0 PH : 6.00 NUMBER OF CRYSTALS USED : 286 RADIATION SOURCE : JEM3000SFF OPTICS : CRYSTALS TILTED TO MAX : 71.3 DEGREES DETECTOR TYPE : CCD DETECTOR MANUFACTURER : GATAN 2K X 2K DATA SCALING SOFTWARE : GATAN ACCELERATION VOLTAGE (KV) : 300 NUMBER OF UNIQUE REFLECTIONS : 22293 RESOLUTION RANGE HIGH (A) : 1.9 RESOLUTION RANGE LOW (A) : 20.000 OVERALL. COMPLETENESS FOR RANGE (%) : 80.0 DATA REDUNDANCY : 5.700 IN THE HIGHEST RESOLUTION SHELL. HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.90 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 2.0 COMPLETENESS FOR SHELL (%) : 82.0 DATA REDUNDANCY IN SHELL : 5.70 R MERGE FOR SHELL (I) : 0.166 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR : REPLACEMENT SOFTWARE USED: CNS STARTING MODEL: PDB ENTRY 1SOR
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