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Yorodumi- PDB-1f7u: CRYSTAL STRUCTURE OF THE ARGINYL-TRNA SYNTHETASE COMPLEXED WITH T... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f7u | ||||||
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Title | CRYSTAL STRUCTURE OF THE ARGINYL-TRNA SYNTHETASE COMPLEXED WITH THE TRNA(ARG) AND L-ARG | ||||||
Components |
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Keywords | LIGASE/RNA / RNA-protein complex / aminoacylation / Arginyl-tRNA synthetase / ligase / LIGASE-RNA COMPLEX | ||||||
Function / homology | Function and homology information arginine-tRNA ligase / arginine-tRNA ligase activity / arginyl-tRNA aminoacylation / mitochondrial translation / mitochondrion / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.2 Å | ||||||
Authors | Delagoutte, B. / Moras, D. / Cavarelli, J. | ||||||
Citation | Journal: EMBO J. / Year: 2000 Title: tRNA aminoacylation by arginyl-tRNA synthetase: induced conformations during substrates binding Authors: Delagoutte, B. / Moras, D. / Cavarelli, J. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization and preliminary X-ray crystallographic analysis of yeast arginyl-tRNA synthetase-yeast tRNA complexes Authors: Delagoutte, B. / Keith, G. / Moras, D. / Cavarelli, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f7u.cif.gz | 195 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f7u.ent.gz | 147.2 KB | Display | PDB format |
PDBx/mmJSON format | 1f7u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f7/1f7u ftp://data.pdbj.org/pub/pdb/validation_reports/f7/1f7u | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | the biological assembly contains one monomer constructed from chain A and one tRNA (chain B) |
-Components
#1: RNA chain | Mass: 24573.770 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: this sequence occurs naturally in yeast Saccharomyces cerevisae |
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#2: Protein | Mass: 69617.867 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Plasmid: PTRC99 / Production host: Escherichia coli (E. coli) / References: UniProt: Q05506, arginine-tRNA ligase |
#3: Chemical | ChemComp-SO4 / |
#4: Chemical | ChemComp-ARG / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.39 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 2.0 M (NH4)2SO4, hexanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 277K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Details: Delagoutte, B., (2000) Acta Crystallogr., Sect.D, 56, 492. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.9322 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9322 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→15 Å / Num. all: 51596 / Num. obs: 51493 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7 % / Biso Wilson estimate: 14 Å2 / Rmerge(I) obs: 0.089 / Net I/σ(I): 23.4 |
Reflection shell | Resolution: 2.2→2.3 Å / Redundancy: 7 % / Rmerge(I) obs: 0.21 / Num. unique all: 6786 / % possible all: 99.3 |
-Processing
Software |
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Refinement | Resolution: 2.2→14.98 Å / Rfactor Rfree error: 0.005 / Data cutoff high rms absF: 2399277.34 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 44.3048 Å2 / ksol: 0.399864 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→14.98 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 6
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.19 / Rfactor Rwork: 0.19 | ||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 30.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.256 / % reflection Rfree: 5.2 % / Rfactor Rwork: 0.21 |