+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1mhi | ||||||
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タイトル | THREE-DIMENSIONAL SOLUTION STRUCTURE OF AN INSULIN DIMER. A STUDY OF THE B9(ASP) MUTANT OF HUMAN INSULIN USING NUCLEAR MAGNETIC RESONANCE DISTANCE GEOMETRY AND RESTRAINED MOLECULAR DYNAMICS | ||||||
要素 | (INSULIN) x 2 | ||||||
キーワード | HORMONE | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / negative regulation of acute inflammatory response / Regulation of gene expression in beta cells / alpha-beta T cell activation / regulation of cellular amino acid metabolic process / negative regulation of respiratory burst involved in inflammatory response / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of protein secretion / regulation of protein localization to plasma membrane / fatty acid homeostasis / Signal attenuation / negative regulation of lipid catabolic process / negative regulation of gluconeogenesis / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / negative regulation of reactive oxygen species biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / nitric oxide-cGMP-mediated signaling / transport vesicle / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / neuron projection maintenance / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of glycolytic process / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / acute-phase response / Regulation of insulin secretion / negative regulation of protein catabolic process / endosome lumen / positive regulation of glucose import / positive regulation of protein secretion / negative regulation of proteolysis / positive regulation of cell differentiation / regulation of transmembrane transporter activity / insulin receptor binding / wound healing / regulation of synaptic plasticity / vasodilation / hormone activity / cognition / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / glucose metabolic process / regulation of protein localization / glucose homeostasis / insulin receptor signaling pathway / cell-cell signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of NF-kappaB transcription factor activity / positive regulation of cell growth / protease binding / secretory granule lumen / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / Golgi membrane / endoplasmic reticulum lumen / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR | ||||||
データ登録者 | Jorgensen, A.M.M. / Kristensen, S.M. / Led, J.J. / Balschmidt, P. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 1992 タイトル: Three-dimensional solution structure of an insulin dimer. A study of the B9(Asp) mutant of human insulin using nuclear magnetic resonance, distance geometry and restrained molecular dynamics. 著者: Jorgensen, A.M. / Kristensen, S.M. / Led, J.J. / Balschmidt, P. #1: ジャーナル: Magn.Reson.Chem. / 年: 1995 タイトル: A Carbon-13 NMR Study of the B9(Asp) Mutant of Human Insulin 著者: Kristensen, S.M. / Led, J.J. #2: ジャーナル: Biochemistry / 年: 1994 タイトル: Structural Details of Asp(B9) Human Insulin at Low Ph from 2D NMR Titration Studies 著者: Sorensen, M.D. / Led, J.J. #3: ジャーナル: J.Am.Chem.Soc. / 年: 1994 タイトル: A New Linear Prediction Model Method for the Determination of Slow Amide Proton Exchange Rates from a Series of One-Dimensional 1H NMR Spectra 著者: Moss, R. / Gesmar, H. / Led, J.J. #4: ジャーナル: J.Am.Chem.Soc. / 年: 1993 タイトル: Slow Amide Proton Exchange Rates from the Line Widths in a Single Two-Dimensional 1H NMR Spectrum 著者: Olsen, H.B. / Gesmar, H. / Led, J.J. #5: ジャーナル: J.Mol.Biol. / 年: 1991 タイトル: Proton Nuclear Magnetic Resonance Study of the B9(Asp) Mutant of Human Insulin. Sequential Assignment and Secondary Structure 著者: Kristensen, S.M. / Jorgensen, A.M.M. / Led, J.J. / Balschmidt, P. / Hansen, F.B. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1mhi.cif.gz | 343.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1mhi.ent.gz | 298.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1mhi.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1mhi_validation.pdf.gz | 366.2 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1mhi_full_validation.pdf.gz | 652.5 KB | 表示 | |
XML形式データ | 1mhi_validation.xml.gz | 27.5 KB | 表示 | |
CIF形式データ | 1mhi_validation.cif.gz | 42.4 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/mh/1mhi ftp://data.pdbj.org/pub/pdb/validation_reports/mh/1mhi | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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Atom site foot note | 1: THR B 27 - PRO B 28 MODEL 1 OMEGA = 41.00 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 2: THR B 27 - PRO B 28 MODEL 3 OMEGA = 214.06 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 3: THR B 27 - PRO B 28 MODEL 4 OMEGA = 213.37 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 4: THR B 27 - PRO B 28 MODEL 8 OMEGA = 216.24 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 5: THR B 27 - PRO B 28 MODEL 10 OMEGA = 241.03 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 6: THR B 27 - PRO B 28 MODEL 11 OMEGA = 217.65 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 7: THR B 27 - PRO B 28 MODEL 13 OMEGA = 233.42 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 8: CIS PROLINE - PRO B 28 MODEL 15 9: THR B 27 - PRO B 28 MODEL 19 OMEGA = 240.85 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 10: THR B 27 - PRO B 28 MODEL 20 OMEGA = 91.63 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION | |||||||||
NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 1 / 変異: S(B 9)D / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 |
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#2: タンパク質・ペプチド | 分子量: 3461.963 Da / 分子数: 1 / 変異: S(B 9)D / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 |
-実験情報
-実験
実験 | 手法: 溶液NMR |
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-試料調製
結晶化 | *PLUS 手法: other / 詳細: NMR |
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-解析
NMR software |
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NMRアンサンブル | 登録したコンフォーマーの数: 20 |