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Yorodumi- PDB-1vpu: NMR SOLUTION STRUCTURE OF THE HIV-1 VPU CYTOPLASMIC DOMAIN, 9 STR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1vpu | ||||||
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Title | NMR SOLUTION STRUCTURE OF THE HIV-1 VPU CYTOPLASMIC DOMAIN, 9 STRUCTURES | ||||||
Components | VPU PROTEIN | ||||||
Keywords | AIDS / HIV / VPU | ||||||
Function / homology | Function and homology information receptor catabolic process / CD4 receptor binding / host cell membrane / viral release from host cell / monoatomic cation channel activity / suppression by virus of host tetherin activity / lipid binding / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / membrane Similarity search - Function | ||||||
Biological species | Human immunodeficiency virus 1 | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS | ||||||
Authors | Willbold, D. / Hoffmann, S. / Rosch, P. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 1997 Title: Secondary structure and tertiary fold of the human immunodeficiency virus protein U (Vpu) cytoplasmic domain in solution. Authors: Willbold, D. / Hoffmann, S. / Rosch, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1vpu.cif.gz | 125.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1vpu.ent.gz | 107 KB | Display | PDB format |
PDBx/mmJSON format | 1vpu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vp/1vpu ftp://data.pdbj.org/pub/pdb/validation_reports/vp/1vpu | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 5111.440 Da / Num. of mol.: 1 / Fragment: CYTOPLASMIC DOMAIN RESIDUES 39 - 81 / Mutation: R37L, K38Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human immunodeficiency virus 1 / Genus: Lentivirus / Strain: SF162 ISOLATE / Cell line: BL21 / Gene: SYNTHETIC GENE / Plasmid: PET16B (NOVAGEN) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P19554 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: REPRESENTATIVE MODEL NUMBER: 3 |
-Sample preparation
Sample conditions | pH: 7.0 / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS / Software ordinal: 1 | ||||||||
NMR ensemble | Conformer selection criteria: ENERGY, AGREEMENT WITH EXPERIMENTAL DATA Conformers calculated total number: 25 / Conformers submitted total number: 9 |