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Yorodumi- PDB-2kc6: NMR solution structure of the pheromone En-1 of Euplotes nobilii ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kc6 | ||||||
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Title | NMR solution structure of the pheromone En-1 of Euplotes nobilii at -1.5 C | ||||||
Components | Mating pheromone En-1 | ||||||
Keywords | SIGNALING PROTEIN / Pheromone / Secreted | ||||||
Function / homology | Pheromone ER-1 - #40 / Pheromone ER-1 / pheromone activity / Up-down Bundle / extracellular region / Mainly Alpha / Mating pheromone En-1 Function and homology information | ||||||
Biological species | Euplotes nobilii (eukaryote) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, molecular dynamics | ||||||
Authors | Pedrini, B. / Alimenti, C. / Vallesi, A. / Luporini, P. / Wuthrich, K. | ||||||
Citation | Journal: Iubmb Life / Year: 2009 Title: Molecular cold-adaptation: Comparative analysis of two homologous families of psychrophilic and mesophilic signal proteins of the protozoan ciliate, Euplotes. Authors: Alimenti, C. / Vallesi, A. / Pedrini, B. / Wuthrich, K. / Luporini, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kc6.cif.gz | 271.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kc6.ent.gz | 236.8 KB | Display | PDB format |
PDBx/mmJSON format | 2kc6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kc6_validation.pdf.gz | 343 KB | Display | wwPDB validaton report |
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Full document | 2kc6_full_validation.pdf.gz | 428 KB | Display | |
Data in XML | 2kc6_validation.xml.gz | 14.9 KB | Display | |
Data in CIF | 2kc6_validation.cif.gz | 27.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kc/2kc6 ftp://data.pdbj.org/pub/pdb/validation_reports/kc/2kc6 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5630.084 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Euplotes nobilii (eukaryote) / Strain: AC-1 / References: UniProt: P83441 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.0 mM En-1-1, 20 mM sodium phosphate-2, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | |||||||||
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, molecular dynamics / Software ordinal: 1 / Details: Structure calculation, Energy minimization | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 80 / Conformers submitted total number: 20 |