electron transfer activity / iron ion binding / heme binding 類似検索 - 分子機能
Cytochrome c, class IE / Cytochrome C oxidase, cbb3-type, subunit III / Cytochrome c-like domain / Cytochrome Bc1 Complex; Chain D, domain 2 / Cytochrome c family profile. / Cytochrome c-like domain / Cytochrome c-like domain superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
HEME C / Mono-heme c-type cytochrome ScyA 類似検索 - 構成要素
生物種
Shewanella putrefaciens (シェワネラ・プトレファシエンス)
手法
溶液NMR / simulated annealing in the dihedral angle space in dyana in 15000 steps; 30 structures with the lowest target function out of a total number of 250 generated structures constitute the REM family - subject to restrained energy minimization in AMBER
SEQUENCE Recombinant protein, without the signaling peptide. The first three amino-acids (Ala Asp ...SEQUENCE Recombinant protein, without the signaling peptide. The first three amino-acids (Ala Asp Leu) have been added for protein expression purposes.
内容: 1.5 mM Cytochrome c: 100 mM phosphate buffer; reduced with dithyonite 溶媒系: 90% H2O/10% D2O
試料状態
pH: 7.0 / 圧: ambient / 温度: 298 K
結晶化
*PLUS
手法: その他 / 詳細: NMR
-
NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker AVANCE
Bruker
AVANCE
800
1
Bruker AVANCE
Bruker
AVANCE
700
2
Bruker AVANCE
Bruker
AVANCE
600
3
-
解析
NMR software
名称
バージョン
開発者
分類
DYANA
1.5
Guntert, P., Mumenthaler, C., Wuthrich, K.
構造決定
Amber
6
Case
精密化
精密化
手法: simulated annealing in the dihedral angle space in dyana in 15000 steps; 30 structures with the lowest target function out of a total number of 250 generated structures constitute the REM ...手法: simulated annealing in the dihedral angle space in dyana in 15000 steps; 30 structures with the lowest target function out of a total number of 250 generated structures constitute the REM family - subject to restrained energy minimization in AMBER ソフトェア番号: 1
NMRアンサンブル
コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 250 / 登録したコンフォーマーの数: 30