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- PDB-3lfg: Crystal structure of HPr-C-His from Thermoanaerobacter tengcongensis -
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Open data
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Basic information
Entry | Database: PDB / ID: 3lfg | ||||||
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Title | Crystal structure of HPr-C-His from Thermoanaerobacter tengcongensis | ||||||
![]() | Phosphotransferase system, HPr-related proteins | ||||||
![]() | TRANSFERASE / HPr fruB phosphotransfer PTS / Kinase / Phosphotransferase system | ||||||
Function / homology | ![]() Phosphotransferase system, HPr histidine phosphorylation site / PTS HPR domain histidine phosphorylation site signature. / HPr-like / Histidine-containing Protein; Chain: A; / Phosphocarrier protein HPr-like / HPr-like superfamily / PTS HPr component phosphorylation site / PTS HPR domain profile. / 2-Layer Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fu, T.M. / Su, X.D. | ||||||
![]() | ![]() Title: Dimerization of HPr by reversible domain swapping Authors: Fu, T.M. / Su, X.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 115.8 KB | Display | ![]() |
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PDB format | ![]() | 91.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 440.9 KB | Display | ![]() |
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Full document | ![]() | 441.5 KB | Display | |
Data in XML | ![]() | 14.5 KB | Display | |
Data in CIF | ![]() | 20.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10691.167 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: MB4T / Gene: FruB, TTE1820 / Plasmid: pET21b / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 30% PEG 4000, 0.1M NaAc pH 4.6, 0.2M ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jun 2, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 1.58→50 Å / Num. all: 37529 / Num. obs: 37192 / % possible obs: 99.1 % / Redundancy: 7.6 % / Biso Wilson estimate: 17.36 Å2 / Rsym value: 0.116 |
Reflection shell | Resolution: 1.58→1.61 Å / Redundancy: 4.7 % / Num. unique all: 1683 / % possible all: 91.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 65.793 Å2 / ksol: 0.383 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 62.92 Å2 / Biso mean: 19.972 Å2 / Biso min: 8.63 Å2
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Refinement step | Cycle: LAST / Resolution: 1.58→28.16 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 12
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Refinement TLS params. | Method: refined / Origin x: 11.16 Å / Origin y: -10.8294 Å / Origin z: 11.0281 Å
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Refinement TLS group |
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