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- PDB-3le3: Crystal structure of apoHPr monomer from Thermoanaerobacter tengc... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3le3 | ||||||
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Title | Crystal structure of apoHPr monomer from Thermoanaerobacter tengcongensis | ||||||
![]() | Phosphotransferase system, HPr-related proteins | ||||||
![]() | TRANSFERASE / HPr PTS phosphotransferase / Kinase / Phosphotransferase system | ||||||
Function / homology | ![]() Phosphotransferase system, HPr histidine phosphorylation site / PTS HPR domain histidine phosphorylation site signature. / HPr-like / Histidine-containing Protein; Chain: A; / Phosphocarrier protein HPr-like / HPr-like superfamily / PTS HPr component phosphorylation site / PTS HPR domain profile. / 2-Layer Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fu, T.M. / Su, X.D. | ||||||
![]() | ![]() Title: Dimerization of HPr by reversible domain swapping Authors: Fu, T.M. / Su, X.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.5 KB | Display | ![]() |
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PDB format | ![]() | 49.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 425 KB | Display | ![]() |
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Full document | ![]() | 425.2 KB | Display | |
Data in XML | ![]() | 14.2 KB | Display | |
Data in CIF | ![]() | 20.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9620.015 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: MB4T / Gene: FruB, TTE1820 / Plasmid: pET21b / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.82 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.05M MgCl2, 0.1M HEPES pH7.5, 30% PEG MME 550, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Nov 26, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9797 Å / Relative weight: 1 |
Reflection | Resolution: 1.86→50 Å / Num. all: 23136 / Num. obs: 22049 / % possible obs: 95.3 % / Redundancy: 7.2 % / Biso Wilson estimate: 21.98 Å2 / Rsym value: 0.034 |
Reflection shell | Resolution: 1.86→1.89 Å / Redundancy: 5.8 % / Num. unique all: 1045 / % possible all: 91.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.562 Å2 / ksol: 0.325 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.922 Å2
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Refinement step | Cycle: LAST / Resolution: 1.861→30.275 Å
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Refine LS restraints |
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LS refinement shell |
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