+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1jca | ||||||
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タイトル | Non-standard Design of Unstable Insulin Analogues with Enhanced Activity | ||||||
要素 |
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キーワード | HORMONE/GROWTH FACTOR / A8-Lysine human insulin / insulin receptor (インスリン受容体) / HORMONE-GROWTH FACTOR COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of dendritic spine maintenance / alpha-beta T cell activation / negative regulation of acute inflammatory response / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / fatty acid homeostasis / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / positive regulation of lipid biosynthetic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / positive regulation of nitric oxide mediated signal transduction / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of lipid catabolic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / negative regulation of reactive oxygen species biosynthetic process / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / Regulation of insulin secretion / positive regulation of nitric-oxide synthase activity / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of protein secretion / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of glucose import / negative regulation of proteolysis / regulation of synaptic plasticity / wound healing / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of neuron projection development / hormone activity / 認識 / Golgi lumen / vasodilation / positive regulation of protein localization to nucleus / glucose metabolic process / regulation of protein localization / glucose homeostasis / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
手法 | X線回折 / フーリエ合成 / 解像度: 2.5 Å | ||||||
データ登録者 | Weiss, M.A. / Wan, Z. / Zhao, M. / Chu, Y.-C. / Nakagawa, S.H. / Burke, G.T. / Jia, W. / Hellmich, R. / Katsoyannis, P.G. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 2002 タイトル: Non-standard insulin design: structure-activity relationships at the periphery of the insulin receptor. 著者: Weiss, M.A. / Wan, Z. / Zhao, M. / Chu, Y.C. / Nakagawa, S.H. / Burke, G.T. / Jia, W. / Hellmich, R. / Katsoyannis, P.G. #1: ジャーナル: BIOPHYS.CHEM. / 年: 1994 タイトル: A Proposed Interaction Model of Insulin Molecules with its Receptor 著者: Liang, D.C. / Chang, W.R. / Wan, Z.L. / Vijayan, N.M. #2: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 2000 タイトル: Diabetes-associated Mutations in a beta-Cell Transcription Factor Destabilize an Antiparallel "mini-zipper" in a Dimerization Interface 著者: Hua, Q.X. / Zhao, M. / Narayana, N. / Nakagawa, S.H. / Jia, W. / Weiss, M.A. #3: ジャーナル: J.Mol.Biol. / 年: 1998 タイトル: The Relationship Between Insulin Bioactivity and Structure in the NH2-terminal A-chain Helix 著者: Olsen, H.B. / Ludvigsen, S. / Kaarsholm, N.C. #4: ジャーナル: J.Mol.Biol. / 年: 1996 タイトル: Mapping the Functional Surface of Insulin by Design: Structure and Function of Novel A-chain Analogue 著者: Hua, Q.X. / Hu, S.Q. / Frank, B.H. / Jia, W. / Chu, Y.C. / Wang, S.H. / Burke, G.T. / Katsoyannis, P.G. / Weiss, M.A. | ||||||
履歴 |
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Remark 400 | COMPOUND The crystallographic asymmetric unit of insulin consists of two insulin monomers each ...COMPOUND The crystallographic asymmetric unit of insulin consists of two insulin monomers each consisting of two heterochains. The entry presents coordinates for monomer 1 (chain indicators A and B) and monomer 2 (chain indicators C and D). There are two zinc ions per insulin hexamer located on the three-fold axis. The conformations of two monomers are different as the result of a change in conformation of the first eight residues of the B-chain. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1jca.cif.gz | 33.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1jca.ent.gz | 23.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1jca.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jc/1jca ftp://data.pdbj.org/pub/pdb/validation_reports/jc/1jca | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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2 |
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3 |
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単位格子 |
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Components on special symmetry positions |
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詳細 | The biological assembly is a hexamer generated from the dimer in the asymmetric unit by the operations: -y, x-y, z and -x+y, -x, z. |
-要素
#1: タンパク質・ペプチド | 分子量: 2411.774 Da / 分子数: 2 / 変異: T8K / 由来タイプ: 合成 詳細: This peptide was chemically synthesized. The sequence of the peptide is naturally found in Homo sapiens (humans). 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 2 / 由来タイプ: 合成 詳細: This peptide was chemically synthesized. The sequence of the peptide is naturally found in Homo sapiens (humans). 参照: UniProt: P01308 #3: 化合物 | #4: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.03 Å3/Da / 溶媒含有率: 39.43 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: Tris, sodium citrate, acetone, phenol, pH 7.0, VAPOR DIFFUSION, HANGING DROP Temp details: ambient | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 7.8 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 290 K |
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放射光源 | 由来: 回転陽極 / タイプ: RIGAKU RU200 / 波長: 1.5418 Å |
検出器 | タイプ: RIGAKU RAXIS II / 検出器: IMAGE PLATE / 日付: 2000年1月1日 / 詳細: mirrors |
放射 | モノクロメーター: MIRROR / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.5→10 Å / Num. all: 3369 / Num. obs: 3169 / % possible obs: 99.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / 冗長度: 2 % / Rmerge(I) obs: 0.071 |
反射 シェル | 解像度: 2.5→2.59 Å / Rmerge(I) obs: 0.16 / % possible all: 97.5 |
反射 | *PLUS Rmerge(I) obs: 0.076 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: フーリエ合成 開始モデル: PDB ENTRY 1TRZ 解像度: 2.5→10 Å / σ(F): 2 / σ(I): 1
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精密化ステップ | サイクル: LAST / 解像度: 2.5→10 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: X-PLOR / バージョン: 3.1 / 分類: refinement | |||||||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.5 Å / 最低解像度: 10 Å / σ(F): 2 / Rfactor obs: 0.204 | |||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||
原子変位パラメータ | *PLUS | |||||||||||||||||||||||||
拘束条件 | *PLUS
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