- EMDB-10153: Class2A : CENP-A nucleosome in complex with CENP-C central region -
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基本情報
登録情報
データベース: EMDB / ID: EMD-10153
タイトル
Class2A : CENP-A nucleosome in complex with CENP-C central region
マップデータ
試料
CENP-A nucleosome in complex with CENP-C central domain: Nucleosomeヌクレオソーム / DNAデオキシリボ核酸 / Histone H3-like centromeric protein A / Histone H4 / Histone H2A type 2-A / Histone H2B type 1-C/E/F/G/I / (nucleic-acid核酸) x 2 / Centromere protein Cセントロメア
機能・相同性
機能・相同性情報
monopolar spindle attachment to meiosis I kinetochore / centromeric DNA binding / condensed chromosome inner kinetochore / attachment of mitotic spindle microtubules to kinetochore / kinetochore assembly / protein localization to chromosome, centromeric region / pericentric heterochromatin => GO:0005721 / condensed nuclear chromosome kinetochore / condensed chromosome, centromeric region => GO:0000779 / establishment of mitotic spindle orientation ...monopolar spindle attachment to meiosis I kinetochore / centromeric DNA binding / condensed chromosome inner kinetochore / attachment of mitotic spindle microtubules to kinetochore / kinetochore assembly / protein localization to chromosome, centromeric region / pericentric heterochromatin => GO:0005721 / condensed nuclear chromosome kinetochore / condensed chromosome, centromeric region => GO:0000779 / establishment of mitotic spindle orientation / negative regulation of megakaryocyte differentiation / pericentric heterochromatin / chromosome, centromeric region / CENP-A containing nucleosome assembly / DNA replication-independent nucleosome assembly / mitotic cytokinesis / telomere capping / chromatin silencing / telomere organization / DNA replication-dependent nucleosome assembly / nucleosome => GO:0000786 / 動原体 / innate immune response in mucosa / rDNA heterochromatin assembly / negative regulation of gene expression, epigenetic / regulation of gene silencing by miRNA / nuclear chromosome / DNA-templated transcription, initiation / chromosome segregation / regulation of megakaryocyte differentiation / nucleosome assembly / ヌクレオソーム / midbody / mitotic cell cycle / double-strand break repair via nonhomologous end joining / chromatin organization / chromosome, telomeric region => GO:0000781 / antibacterial humoral response / nuclear body / antimicrobial humoral immune response mediated by antimicrobial peptide / protein ubiquitination / protein heterodimerization activity / defense response to Gram-positive bacterium / 細胞分裂 / chromatin => GO:0000785 / protein domain specific binding / cellular protein metabolic process / chromatin binding / viral process / protein-containing complex / RNA binding / DNA binding / extracellular space / extracellular exosome / 生体膜 / extracellular region / 核質 / identical protein binding / 細胞核 / 細胞質基質
Histone H4 / Histone H2B / Histone H2A / TATA box binding protein associated factor (TAF) / Histone H2A/H2B/H3 / Histone-fold / RmlC-like cupin domain superfamily / RmlC-like jelly roll fold / Histone H4, conserved site / Mif2/CENP-C cupin domain ...Histone H4 / Histone H2B / Histone H2A / TATA box binding protein associated factor (TAF) / Histone H2A/H2B/H3 / Histone-fold / RmlC-like cupin domain superfamily / RmlC-like jelly roll fold / Histone H4, conserved site / Mif2/CENP-C cupin domain / Kinetochore assembly subunit CENP-C, N-terminal domain / Histone H3/CENP-A / Centromere protein C/Mif2/cnp3 / CENP-C, middle DNMT3B-binding domain / Histone H2A, C-terminal domain / Histone H2A conserved site / CENP-T/Histone H4, histone fold
Histone H3-like centromeric protein A / Histone H4 / Histone H2B type 1-C/E/F/G/I / Centromere protein C / Histone H2A type 2-A
ジャーナル: EMBO Rep / 年: 2019 タイトル: CENP-C unwraps the human CENP-A nucleosome through the H2A C-terminal tail. 著者: Ahmad Ali-Ahmad / Silvija Bilokapić / Ingmar B Schäfer / Mario Halić / Nikolina Sekulić / 要旨: Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. CENP-C is ...Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. CENP-C is considered to be a central organizer of the CCAN. We provide new molecular insights into the structure of human CENP-A nucleosomes, in isolation and in complex with the CENP-C central region (CENP-C ), the main CENP-A binding module of human CENP-C. We establish that the short αN helix of CENP-A promotes DNA flexibility at the nucleosome ends, independently of the sequence it wraps. Furthermore, we show that, in vitro, two regions of human CENP-C (CENP-C and CENP-C ) both bind exclusively to the CENP-A nucleosome. We find CENP-C to bind with high affinity due to an extended hydrophobic area made up of CENP-A and CENP-A . Importantly, we identify two key conformational changes within the CENP-A nucleosome upon CENP-C binding. First, the loose DNA wrapping of CENP-A nucleosomes is further exacerbated, through destabilization of the H2A C-terminal tail. Second, CENP-C rigidifies the N-terminal tail of H4 in the conformation favoring H4 monomethylation, essential for a functional centromere.
全体 CENP-A nucleosome in complex with CENP-C central domain
全体
名称: CENP-A nucleosome in complex with CENP-C central domain 構成要素数: 10
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構成要素 #1: タンパク質, CENP-A nucleosome in complex with CENP-C central domain
タンパク質
名称: CENP-A nucleosome in complex with CENP-C central domain 組換発現: No
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構成要素 #2: タンパク質, Nucleosome
タンパク質
名称: Nucleosomeヌクレオソーム / 組換発現: No
由来
生物種: Homo sapiens (ヒト)
由来(合成)
発現系: Escherichia coli (大腸菌)
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構成要素 #3: タンパク質, DNA
タンパク質
名称: DNAデオキシリボ核酸 / 組換発現: No
由来
生物種: synthetic construct (人工物)
由来(合成)
発現系: Escherichia coli (大腸菌)
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構成要素 #4: タンパク質, Histone H3-like centromeric protein A
タンパク質
名称: Histone H3-like centromeric protein A / 個数: 2 / 組換発現: No
分子量
理論値: 16.02363 kDa
由来
生物種: Homo sapiens (ヒト)
由来(合成)
発現系: Escherichia coli (大腸菌)
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構成要素 #5: タンパク質, Histone H4
タンパク質
名称: Histone H4 / 個数: 2 / 組換発現: No
分子量
理論値: 11.394426 kDa
由来
生物種: Homo sapiens (ヒト)
由来(合成)
発現系: Escherichia coli (大腸菌)
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構成要素 #6: タンパク質, Histone H2A type 2-A
タンパク質
名称: Histone H2A type 2-A / 個数: 2 / 組換発現: No
分子量
理論値: 14.125549 kDa
由来
生物種: Homo sapiens (ヒト)
由来(合成)
発現系: Escherichia coli (大腸菌)
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構成要素 #7: タンパク質, Histone H2B type 1-C/E/F/G/I
タンパク質
名称: Histone H2B type 1-C/E/F/G/I / 個数: 2 / 組換発現: No
分子量
理論値: 13.937213 kDa
由来
生物種: Homo sapiens (ヒト)
由来(合成)
発現系: Escherichia coli (大腸菌)
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構成要素 #8: nucleic-acid, DNA (145-MER)
核酸
名称: DNA (145-MER) / クラス: DNA / Structure: OTHER / 合成: No 配列: (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT) (DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT) ...配列:
名称: DNA (145-MER) / クラス: DNA / Structure: OTHER / 合成: No 配列: (DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DA)(DT) (DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA) (DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA) (DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG) (DT)(DA)(DA)(DT)(DC)(DC)(DC) ...配列: