+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDAB4 |
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試料 | Truncated GbpA
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生物種 | Vibrio cholerae (コレラ菌) |
引用 | ジャーナル: PLoS Pathog / 年: 2012 タイトル: The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. 著者: Edmond Wong / Gustav Vaaje-Kolstad / Avishek Ghosh / Ramon Hurtado-Guerrero / Peter V Konarev / Adel F M Ibrahim / Dmitri I Svergun / Vincent G H Eijsink / Nabendu S Chatterjee / Daan M F van Aalten / 要旨: Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N- ...Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N-acetylglucosamine binding protein (GbpA) that has been reported to mediate bacterial attachment to both marine chitin and mammalian intestinal mucin through an unknown molecular mechanism. We report structural studies that reveal that GbpA possesses an unusual, elongated, four-domain structure, with domains 1 and 4 showing structural homology to chitin binding domains. A glycan screen revealed that GbpA binds to GlcNAc oligosaccharides. Structure-guided GbpA truncation mutants show that domains 1 and 4 of GbpA interact with chitin in vitro, whereas in vivo complementation studies reveal that domain 1 is also crucial for mucin binding and intestinal colonization. Bacterial binding studies show that domains 2 and 3 bind to the V. cholerae surface. Finally, mouse virulence assays show that only the first three domains of GbpA are required for colonization. These results explain how GbpA provides structural/functional modular interactions between V. cholerae, intestinal epithelium and chitinous exoskeletons. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #53 | タイプ: dummy / ソフトウェア: Gasbor / カイ2乗値: 2.6569 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #54 | タイプ: atomic / ソフトウェア: Sasref / カイ2乗値: 3.794704 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: Truncated GbpA / Sample MW: 44 kDa / 試料濃度: 2 mg/ml |
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バッファ | 名称: 25 mM Tris/HCl / pH: 7.5 / 組成: NaCl 150.000 mM |
要素 #51 | 名称: GbpA_truncated / タイプ: protein / 記述: Truncated GbpA / 分子量: 44 / 分子数: 1 / 由来: Vibrio cholerae 配列: HGYVSAVENG VAEGRVTLCK FAANGTGEKN THCGAIQYEP QSVEGPDGFP VTGPRDGKIA SAESALAAAL DEQTADRWVK RPIQAGPQTF EWTFTANHVT KDWKYYITKP NWNPNQPLSR DAFDLNPFCV VEGNMVQPPK RVSHECIVPE REGYQVILAV WDVGDTAASF ...配列: HGYVSAVENG VAEGRVTLCK FAANGTGEKN THCGAIQYEP QSVEGPDGFP VTGPRDGKIA SAESALAAAL DEQTADRWVK RPIQAGPQTF EWTFTANHVT KDWKYYITKP NWNPNQPLSR DAFDLNPFCV VEGNMVQPPK RVSHECIVPE REGYQVILAV WDVGDTAASF YNVIDVKFDG NGPVLPDWNP AGQIIPSMDL SIGDTVYTRV FDNDGENPAY RTELKIDSET LTKANQWSYA LATKINQTQK QQRAGQLNGD QFVPVYGTNP IYLKEGSGLK SVEIGYQIEA PQPEYSLTVS GLAKEYEIGE QPIQLDLTLE AQGEMSAELT VYNHHQKPLA SWSQAMTDGE LKSITLELSE AKAGHHMLVS RIKDRDGNLQ DQQTLDFMLV EPQTPPTPGD YDFVFPNGLK EYV |
-実験情報
ビーム | 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron | |||||||||||||||||||||||||||
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検出器 | 名称: MAR 345 Image Plate | |||||||||||||||||||||||||||
スキャン | タイトル: Truncated GbpA / 測定日: 2007年10月16日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 120 sec. / フレーム数: 1 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 512 /
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結果 | カーブのタイプ: single_conc /
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