Full length GbpA (protein), GbpA_full, Vibrio cholerae
Biological species
Vibrio cholerae (bacteria)
Citation
Journal: PLoS Pathog / Year: 2012 Title: The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. Authors: Edmond Wong / Gustav Vaaje-Kolstad / Avishek Ghosh / Ramon Hurtado-Guerrero / Peter V Konarev / Adel F M Ibrahim / Dmitri I Svergun / Vincent G H Eijsink / Nabendu S Chatterjee / Daan M F van Aalten / Abstract: Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N- ...Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N-acetylglucosamine binding protein (GbpA) that has been reported to mediate bacterial attachment to both marine chitin and mammalian intestinal mucin through an unknown molecular mechanism. We report structural studies that reveal that GbpA possesses an unusual, elongated, four-domain structure, with domains 1 and 4 showing structural homology to chitin binding domains. A glycan screen revealed that GbpA binds to GlcNAc oligosaccharides. Structure-guided GbpA truncation mutants show that domains 1 and 4 of GbpA interact with chitin in vitro, whereas in vivo complementation studies reveal that domain 1 is also crucial for mucin binding and intestinal colonization. Bacterial binding studies show that domains 2 and 3 bind to the V. cholerae surface. Finally, mouse virulence assays show that only the first three domains of GbpA are required for colonization. These results explain how GbpA provides structural/functional modular interactions between V. cholerae, intestinal epithelium and chitinous exoskeletons.
Contact author
Petr Konarev (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)
Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron
Detector
Name: MAR 345 Image Plate
Scan
Title: Full length GbpA / Measurement date: Oct 16, 2007 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 120 sec. / Number of frames: 2 / Unit: 1/nm /
Min
Max
Q
0.1656
4.896
Result
D max: 14.5 / Type of curve: single_conc /
Experimental
Porod
MW
60 kDa
-
Volume
-
100 nm3
P(R)
Guinier
Forward scattering, I0
167
160
Radius of gyration, Rg
4 nm
3.9 nm
+
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