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-基本情報
登録情報 | データベース: SASBDB / ID: SASDD55 |
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試料 | Type II toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA
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機能・相同性 | 機能・相同性情報 |
生物種 | Burkholderia pseudomallei (類鼻疽菌) |
引用 | ジャーナル: J Biol Chem / 年: 2018 タイトル: The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. 著者: Ashley J Winter / Christopher Williams / Michail N Isupov / Hannah Crocker / Mariya Gromova / Philip Marsh / Oliver J Wilkinson / Mark S Dillingham / Nicholas J Harmer / Richard W Titball / Matthew P Crump / 要旨: Toxin-antitoxin (TA) systems are present in many bacteria and play important roles in bacterial growth, physiology, and pathogenicity. Those that are best studied are the type II TA systems, in which ...Toxin-antitoxin (TA) systems are present in many bacteria and play important roles in bacterial growth, physiology, and pathogenicity. Those that are best studied are the type II TA systems, in which both toxins and antitoxins are proteins. The HicAB system is one of the prototypic TA systems, found in many bacterial species. Complex interactions between the protein toxin (HicA), the protein antitoxin (HicB), and the DNA upstream of the encoding genes regulate the activity of this system, but few structural details are available about how HicA destabilizes the HicB-DNA complex. Here, we determined the X-ray structures of HicB and the HicAB complex to 1.8 and 2.5 Å resolution, respectively, and characterized their DNA interactions. This revealed that HicB forms a tetramer and HicA and HicB form a heterooctameric complex that involves structural reorganization of the C-terminal (DNA-binding) region of HicB. Our observations indicated that HicA has a profound impact on binding of HicB to DNA sequences upstream of in a stoichiometric-dependent way. At low ratios of HicA:HicB, there was no effect on DNA binding, but at higher ratios, the affinity for DNA declined cooperatively, driving dissociation of the HicA:HicB:DNA complex. These results reveal the structural mechanisms by which HicA de-represses the HicB-DNA complex. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #1881 | タイプ: atomic / カイ2乗値: 2.87828639506 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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-試料
試料 | 名称: Type II toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA 試料濃度: 5 mg/ml / Entity id: 1010 / 1011 |
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バッファ | 名称: 25 mM Tris 150 mM NaCl / pH: 7.5 |
要素 #1010 | 名称: HicB / タイプ: protein 記述: Type II toxin-antitoxin system HicB family antitoxin 分子量: 15.739 / 分子数: 4 / 由来: Burkholderia pseudomallei / 参照: UniProt: Q63NA5 配列: MMEFPIAVHK DDGSVYGVTV PDIPGVHSWG ETIDDAIKNT REAIVGHVET LIELGEDVEF TCSTVEELVA KPEYAGAVWA LVSVDLSQLD SKPERINVSI PRFVLHKIDA YVASRHETRS GFLARAALEA LNEGKKHHHH HH |
要素 #1011 | 名称: HicA / タイプ: protein / 記述: Addiction module toxin, HicA / 分子量: 7.052 / 分子数: 1 / 由来: Burkholderia pseudomallei / 参照: UniProt: Q63NA6 配列: GIDPFTNSSK LIRMLEEDGW RLVRVTGSAH HFKHPKKPGL VTVPHPKKDL PIGTVKSIQK SAGL |
-実験情報
ビーム | 設備名称: Diamond Light Source B21 / 地域: Oxfordshire / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.014 mm | |||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | |||||||||||||||||||||||||||||||||
スキャン | タイトル: Type II toxin-antitoxin system HicB family antitoxin, HicB, bound to addiction module toxin, HicA 測定日: 2016年7月27日 / 保管温度: 4 °C / セル温度: 25 °C / 照射時間: 3 sec. / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 1036 /
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結果 | カーブのタイプ: sec コメント: Analysis of the data involved ScÅtter (www.bioisis.net/). The resultant curve was fit with the crystal structure of HicB, PDB Code: 6G26.
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