+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDAP5 |
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試料 | Native complex CytC_Adr
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生物種 | Escherichia coli (大腸菌) |
引用 | ジャーナル: J Am Chem Soc / 年: 2008 タイトル: Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy. 著者: Xingfu Xu / Wolfgang Reinle / Frank Hannemann / Peter V Konarev / Dmitri I Svergun / Rita Bernhardt / Marcellus Ubbink / 要旨: In the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the ...In the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the encounter state can represent a significant population of the complex. The redox proteins adrenodoxin (Adx) and cytochrome c (C c) associate to form such a weak and short-lived complex, which is nevertheless active in electron transfer. To study the conformational freedom within the protein complex, the native complex has been compared to a cross-linked counterpart by using solution scattering and NMR spectroscopy. Oligomerization behavior of the native complex in solution revealed by small-angle X-ray scattering indicates a stochastic nature of complex formation. For the cross-linked complex, interprotein paramagnetic effects are observed, whereas for the native complex, extensive averaging occurs, consistent with multiple orientations of the proteins within the complex. Simulations show that C c samples about half of the surface area of adrenodoxin. It is concluded that the complex of Adx/C c is entirely dynamic and can be considered as a pure encounter complex. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #133 | タイプ: dummy / ソフトウェア: Dammin / ダミー原子の半径: 1.90 A / カイ2乗値: 24.000201 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #134 | タイプ: mix / ソフトウェア: Sasref / ダミー原子の半径: 1.90 A / カイ2乗値: 3.8809 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: Native complex CytC_Adr / Sample MW: 44 kDa / 試料濃度: 2.40-24.00 / Entity id: 110 / 111 |
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バッファ | 名称: HEPES / 濃度: 20.00 mM / pH: 7.4 / 組成: DTT 2.000 mM |
要素 #110 | 名称: Cyt_C_dimer / タイプ: protein / 記述: Cytochrome C dimer / 分子量: 11 / 分子数: 2 / 由来: Escherichia coli 配列: TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE |
要素 #111 | 名称: Adrenodoxin dimer / タイプ: protein / 記述: Adrenodoxin dimer / 分子量: 11 / 分子数: 2 / 由来: Escherichia coli 配列: KITVHFINRD GETLTTKGKI GDSLLDVVVQ NNLDIDGFGA CEGTLACSTC HLIFEQHIFE KLEEAITDEE NDMLDLAYGL TDDRSRLGCQ ICLTKAMDNM TVRVP |
-実験情報
ビーム | 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotronシンクロトロン | |||||||||||||||||||||
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検出器 | 名称: MAR 345 Image Plate | |||||||||||||||||||||
スキャン | タイトル: Native complex (Cyt_C_Adr) / 測定日: 2006年6月15日 / 保管温度: 15 °C / セル温度: 15 °C / 照射時間: 120 sec. / フレーム数: 2 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 512 /
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結果 | D max: 9.5 / カーブのタイプ: merged
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