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TitleDynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy.
Journal, issue, pagesJ Am Chem Soc, Vol. 130, Issue 20, Page 6395-6403, Year 2008
Publish dateMay 21, 2008
AuthorsXingfu Xu / Wolfgang Reinle / Frank Hannemann / Peter V Konarev / Dmitri I Svergun / Rita Bernhardt / Marcellus Ubbink /
PubMed AbstractIn the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the ...In the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the encounter state can represent a significant population of the complex. The redox proteins adrenodoxin (Adx) and cytochrome c (C c) associate to form such a weak and short-lived complex, which is nevertheless active in electron transfer. To study the conformational freedom within the protein complex, the native complex has been compared to a cross-linked counterpart by using solution scattering and NMR spectroscopy. Oligomerization behavior of the native complex in solution revealed by small-angle X-ray scattering indicates a stochastic nature of complex formation. For the cross-linked complex, interprotein paramagnetic effects are observed, whereas for the native complex, extensive averaging occurs, consistent with multiple orientations of the proteins within the complex. Simulations show that C c samples about half of the surface area of adrenodoxin. It is concluded that the complex of Adx/C c is entirely dynamic and can be considered as a pure encounter complex.
External linksJ Am Chem Soc / PubMed:18439013
MethodsSAS (X-ray synchrotron) / NMR (solution)
Structure data

SASDAN5:
Cross-linked complex CytC_Adr (Cytochrome C, Cyt_C + Adrenodoxin)
Method: SAXS/SANS

SASDAP5:
Native complex CytC_Adr (Cytochrome C dimer, Cyt_C_dimer + Adrenodoxin dimer)
Method: SAXS/SANS

PDB-2jqr:
Solution model of crosslinked complex of cytochrome c and adrenodoxin
Method: SOLUTION NMR

Chemicals

ChemComp-HEC:
HEME C

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Source
  • Escherichia coli (E. coli)
  • saccharomyces cerevisiae (brewer's yeast)
  • bos taurus (cattle)
KeywordsELECTRON TRANSPORT / Cytochrome c / Adrenodoxin / Crosslinked complex / 2Fe2S Ferredoxin / Pseudocontact shift / Paramagnetic relaxation enhancement / encounter complex

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