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- PDB-7spo: Crystal structure of the SARS-CoV-2 receptor binding domain in co... -

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Basic information

Entry
Database: PDB / ID: 7spo
TitleCrystal structure of the SARS-CoV-2 receptor binding domain in complex with VNAR 3B4
Components
  • Spike protein S1
  • VNAR 3B4
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / membrane / identical protein binding / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
Biological speciesSevere acute respiratory syndrome coronavirus 2
Squalus acanthias (spiny dogfish)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.92 Å
AuthorsShi, K. / Aihara, H.
Funding support United States, China, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM118047 United States
National Natural Science Foundation of China (NSFC)31470160 China
CitationJournal: Nat Commun / Year: 2021
Title: Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography.
Authors: Ubah, O.C. / Lake, E.W. / Gunaratne, G.S. / Gallant, J.P. / Fernie, M. / Robertson, A.J. / Marchant, J.S. / Bold, T.D. / Langlois, R.A. / Matchett, W.E. / Thiede, J.M. / Shi, K. / Yin, L. / ...Authors: Ubah, O.C. / Lake, E.W. / Gunaratne, G.S. / Gallant, J.P. / Fernie, M. / Robertson, A.J. / Marchant, J.S. / Bold, T.D. / Langlois, R.A. / Matchett, W.E. / Thiede, J.M. / Shi, K. / Yin, L. / Moeller, N.H. / Banerjee, S. / Ferguson, L. / Kovaleva, M. / Porter, A.J. / Aihara, H. / LeBeau, A.M. / Barelle, C.J.
History
DepositionNov 2, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 5, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Spike protein S1
B: Spike protein S1
C: VNAR 3B4
D: VNAR 3B4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)80,79813
Polymers79,2224
Non-polymers1,5769
Water4,450247
1
A: Spike protein S1
C: VNAR 3B4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3686
Polymers39,6112
Non-polymers7574
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Spike protein S1
D: VNAR 3B4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,4307
Polymers39,6112
Non-polymers8195
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)81.144, 90.740, 122.692
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 444 through 527 or resid 601))
d_2ens_1(chain "B" and (resid 444 through 527 or resid 603))
d_1ens_2(chain "C" and (resid 21 through 58 or resid 65 through 83 or resid 85 through 134))
d_2ens_2(chain "D" and (resid 21 through 58 or resid 65 through 83 or resid 85 through 134))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1LYSPROA111 - 194
d_12ens_1EDOEDOB
d_21ens_1LYSPROF111 - 194
d_22ens_1EDOEDOI
d_11ens_2SERLYSL1 - 38
d_12ens_2TRPLYSL45 - 63
d_13ens_2PHEVALL65 - 114
d_21ens_2SERLYSN1 - 38
d_22ens_2TRPLYSN44 - 62
d_23ens_2PHEVALN64 - 113

NCS ensembles :
ID
ens_1
ens_2

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Components

#1: Protein Spike protein S1


Mass: 24003.848 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#2: Protein VNAR 3B4


Mass: 15607.319 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Squalus acanthias (spiny dogfish) / Production host: Homo sapiens (human)
#3: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C2H6O2
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 247 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.85 Å3/Da / Density % sol: 56.87 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M Sodium acetate trihydrate, 20 % w/v PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
DetectorType: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Dec 15, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 1.92→81.14 Å / Num. obs: 69770 / % possible obs: 99.9 % / Redundancy: 6.7 % / Biso Wilson estimate: 47.87 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.046 / Rpim(I) all: 0.019 / Rrim(I) all: 0.049 / Net I/σ(I): 19.6 / Num. measured all: 465377
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
1.92-1.966.91.9813067744430.5290.8042.14199.9
9.21-81.146.10.02244617350.9950.010.02465.499.8

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Processing

Software
NameVersionClassification
PHENIX1.20rc3_4406refinement
Aimless0.7.4data scaling
PDB_EXTRACT3.27data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6YM0,4HGK

6ym0

4hgk


Resolution: 1.92→72.96 Å / SU ML: 0.2496 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.1463
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2263 3510 5.04 %
Rwork0.1904 66157 -
obs0.1921 69667 99.79 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 62.22 Å2
Refinement stepCycle: LAST / Resolution: 1.92→72.96 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4900 0 104 247 5251
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01035137
X-RAY DIFFRACTIONf_angle_d0.91116964
X-RAY DIFFRACTIONf_chiral_restr0.0606763
X-RAY DIFFRACTIONf_plane_restr0.0085891
X-RAY DIFFRACTIONf_dihedral_angle_d12.64361846
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.847146118285
ens_2d_2LX-RAY DIFFRACTIONTorsion NCS0.556800428732
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.92-1.950.36831500.34992574X-RAY DIFFRACTION99.78
1.95-1.970.33861750.3152607X-RAY DIFFRACTION99.86
1.97-20.31871270.28542595X-RAY DIFFRACTION99.74
2-2.030.30221510.26342617X-RAY DIFFRACTION99.78
2.03-2.070.27051390.25862603X-RAY DIFFRACTION99.75
2.07-2.10.31071360.26962627X-RAY DIFFRACTION99.75
2.1-2.140.34221360.27762628X-RAY DIFFRACTION99.68
2.14-2.180.32041320.27382630X-RAY DIFFRACTION99.96
2.18-2.230.27491270.23482631X-RAY DIFFRACTION99.78
2.23-2.280.29681430.25292610X-RAY DIFFRACTION99.75
2.28-2.330.2681440.23032625X-RAY DIFFRACTION99.93
2.33-2.390.22361330.21832647X-RAY DIFFRACTION99.82
2.39-2.450.2521370.20382630X-RAY DIFFRACTION99.75
2.45-2.520.29891280.21142616X-RAY DIFFRACTION99.96
2.52-2.610.26021490.22442624X-RAY DIFFRACTION99.71
2.61-2.70.27331400.22882657X-RAY DIFFRACTION99.96
2.7-2.810.24791380.25652633X-RAY DIFFRACTION99.86
2.81-2.930.29431310.2392670X-RAY DIFFRACTION99.82
2.93-3.090.24891530.21322623X-RAY DIFFRACTION99.57
3.09-3.280.25341380.20312670X-RAY DIFFRACTION99.79
3.28-3.540.19991610.20232659X-RAY DIFFRACTION99.93
3.54-3.890.22131210.17212686X-RAY DIFFRACTION99.86
3.89-4.460.17341270.13372722X-RAY DIFFRACTION99.86
4.46-5.610.16771350.13572729X-RAY DIFFRACTION99.86
5.61-72.960.20181590.17722844X-RAY DIFFRACTION99.37
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.071707778910.5502270160031.961605051673.331420296553.782659681119.01254784022-0.1414435824670.1916619101380.1353229396250.278122702694-0.0143440132886-0.131366199356-0.9391729268390.4793560709640.2294551788960.664779289414-0.162572147063-0.06236845797910.3174860466760.009979554640010.4966973328977.40897280581-31.09424530545.38495140975
26.367952297980.3901043782631.669663186822.55379595403-1.526251140223.280824596790.161796421234-0.740375068818-0.08598645788090.57037498863-0.160288774958-0.048292837011-0.4861135804230.066353321817-0.004462474156450.638327758226-0.0729143220566-0.07684430513930.4138269277210.03267645480680.4315608717756.01763097399-40.744630581217.3667681747
33.917209038350.2231436821910.7229814083271.62370770862-0.6375598952643.4429563539-0.04754545821540.23085071921-0.2625131512890.05424022078580.117798090746-0.113033171087-0.1497010853130.183249507816-0.06918344118870.437062964461-0.0242390730496-0.001278365902360.277989603605-0.03159157113680.4223696837560.914878328214-40.6486730684-1.95398282896
40.3880650039560.0204723983231-1.10378730881.3313053831-3.101527095168.91596621207-0.1966997924830.631609855318-0.352358822225-0.3519143499830.18676910274-0.2538270796180.514931032619-0.0799684025183-0.01418854152780.41183169004-0.1611097740050.01697294524430.605212701482-0.1005801547340.4743111565576.33360272938-41.8006910746-19.0688623211
53.67736606413-1.176805816181.976160550190.773394271283-0.6843810500892.562262222340.00962646175655-0.1112028370950.01446720278580.242800301442-0.0574026034412-0.0951523344979-0.4164351802930.1052546259970.08419231428970.547479164574-0.0772183495627-0.03548545761880.3332008601930.05432639516370.5030725292023.57565807309-38.34647148277.01806327951
63.138954957440.9736697456783.336404005552.61914072630.2842944626413.64937813285-0.122548210110.2052708385510.0818624629721-0.0487804174249-0.2382841748250.578306746004-0.369785629935-1.013390558920.448469926880.3102496814920.022440194399-0.03197337194250.8361525177-0.1044221338490.464183276232.66284874889-24.1902882234-34.1572860714
71.718256405620.521913447816-0.8645173132874.95424290812.020346079684.94310670520.03189731656790.24760759519-0.0866935993191-0.552405173847-0.1509758523930.0135332202684-0.0128366406015-0.8179354645690.133241793070.3540069361570.0517460978136-0.0238704595330.602831579546-0.01888385428650.36996962449211.8751644431-25.3605001016-40.5603246267
83.379038293110.740533916306-1.407026694165.11046395110.4147800058927.440825067270.126288049533-0.2168086365710.09029685471260.545309644697-0.268609025171-0.7648563301770.09646485672670.597368033196-0.1570909785790.379651673650.0219733073172-0.1008975059870.4697375981320.08026013020070.55671668947421.2424035085-22.1692455936-25.0210410914
90.518383679371-0.178384169116-0.5133604591884.062070491160.7870596494493.642480973640.1663788465390.1332946836890.06020153702650.0617124400789-0.3148318017040.0300655894403-0.0726116871771-0.7531970604940.1416064642190.279719485323-0.00327255202239-0.005064450955570.5825029366590.01731810792430.40422114841611.2569149399-18.9301005132-26.409923639
103.06241711208-1.06816481995-4.111919354328.885273109376.339390620538.51592828133-0.231195608438-0.262343123084-0.6803195302480.543513354823-0.2156627173420.1672655902370.748620843018-0.3737691922670.4323015537120.474818800544-0.0478043050984-0.07607592444530.5057673314940.01989748662510.45465363878714.5731462511-30.3935820326-21.2541350329
117.3429890738-0.3718758891714.184194068672.334360747142.143812878715.25290907590.227346437838-0.139151116321-0.3508789426650.664668193331-0.209415163462-0.08709909694340.689251536557-0.399070023794-0.03091996204230.656932504573-0.212013650002-0.01392137474790.4537119461140.01013625261410.41887008650513.8399725102-21.3193414131-4.80271729183
120.7732517960260.308379507074-0.4339518841717.719290229384.271720102023.82649430843-0.01753835349240.273276596948-0.0153451444502-0.591214654125-0.1777049700560.0435375251897-0.272478136187-0.613456559430.2097980522680.3762013652630.05864244530374.2835203566E-50.6520372750780.005307029770010.40053283358710.470258791-22.4213845795-35.5780229038
138.65071397653-0.934955629191-1.955139952383.113828675151.260253907132.281136983490.2438310912860.656922876785-1.04721348337-0.119766478933-0.357000602144-0.1193984793620.316262940326-0.2317670685930.1009332744060.478524862902-0.0936864069678-0.05347679736650.3975142133990.04004802201760.532393696421-17.9087162398-51.425095271610.9948931061
146.05954971205-6.542066592415.662930850477.0051669153-6.103291445285.308613179690.341500983545-0.4064380414380.5772504720580.271314051981-0.573565464636-0.272729693729-0.4419749396390.3215195385090.5900845208190.504366834485-0.0610904047392-0.03251168607010.4476634812620.09483446842980.518776595803-15.463392588-43.270348382517.5728296063
154.44748828221-3.0913895274-0.3397940774773.711057067590.9162599402532.39713535402-0.0141464803164-0.158529936461-0.08072850776340.0187314173526-0.0682412199268-0.3341694669210.114457428225-0.04260856458620.09789635143960.457623998901-0.126512259477-0.04151684089360.440250471420.1142318907350.500583868993-18.4935474246-46.590493643517.9906857424
163.23379057275-0.60565961821-0.9858919912442.070861779961.546591755751.011511996490.179823141099-0.121161642-0.8206885899930.316421669686-0.175920606599-0.146150330760.203267404784-0.108930514116-0.00673343842970.504684363592-0.0808318069131-0.03331644121190.4611027090290.2058593628440.524703861852-13.6389778708-48.482128317117.059653944
172.48059070746-2.29266504313-0.1128311075884.48328065451-0.2218709063291.252543885460.4670738822680.189006525064-0.590944303315-0.196088112166-0.346410373631-0.2398383857830.244238243979-0.191300245857-0.145149723980.472077173136-0.032178124759-0.02976214995420.4293732153360.06310387028310.400442963364-11.8832221534-48.308530541510.3031437943
183.891753580811.763153218720.05867266433264.70853029924-5.201980608516.65386401832-0.769911528479-0.531322711670.1092846109281.214805694850.517124575268-0.453781520071-1.28831935396-0.1365524762130.1262390454780.7571345867890.109414772001-0.1254891203250.37599519767-0.0628568622230.44875314328122.9674886013-3.09468197361-43.3449207714
198.011565529661.916842664611.119943748327.35637469693-3.869427338918.04051145251-0.417309930555-1.1115530823-0.378287502471-0.3402451317460.4766429053570.07908780069920.14375017678-0.707016976472-0.1917136611750.471649800960.003877400339880.02280464025970.4543464292750.04138685510450.47805093218619.8507038067-1.88272405238-48.8506470037
202.916779678472.34410117421-1.211326971194.7542474635-4.981213023645.29621341557-0.531311848174-0.212129926643-0.1944138878790.6970198732750.965127000306-0.272212978289-0.711886086127-0.266639075492-0.2884909910470.5793012329680.09438788164810.0201387530250.511167830776-0.02539525193260.36385865670221.6552111957-8.64907047784-44.3984357225
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 334 through 358 )AA334 - 3581 - 25
22chain 'A' and (resid 359 through 393 )AA359 - 39326 - 60
33chain 'A' and (resid 394 through 459 )AA394 - 45961 - 126
44chain 'A' and (resid 460 through 494 )AA460 - 494127 - 161
55chain 'A' and (resid 495 through 529 )AA495 - 529162 - 196
66chain 'B' and (resid 334 through 353 )BF334 - 3531 - 20
77chain 'B' and (resid 354 through 409 )BF354 - 40921 - 76
88chain 'B' and (resid 410 through 421 )BF410 - 42177 - 88
99chain 'B' and (resid 422 through 459 )BF422 - 45989 - 126
1010chain 'B' and (resid 460 through 469 )BF460 - 469127 - 136
1111chain 'B' and (resid 470 through 494 )BF470 - 494137 - 161
1212chain 'B' and (resid 495 through 528 )BF495 - 528162 - 195
1313chain 'C' and (resid 21 through 51 )CL21 - 511 - 31
1414chain 'C' and (resid 52 through 58 )CL52 - 5832 - 38
1515chain 'C' and (resid 59 through 89 )CL59 - 8939 - 69
1616chain 'C' and (resid 90 through 115 )CL90 - 11570 - 95
1717chain 'C' and (resid 116 through 138 )CL116 - 13896 - 118
1818chain 'D' and (resid 21 through 58 )DN21 - 581 - 38
1919chain 'D' and (resid 59 through 89 )DN59 - 8939 - 68
2020chain 'D' and (resid 90 through 136 )DN90 - 13669 - 115

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