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Yorodumi- PDB-1ta4: Crystal Structure Of Aspartate-Semialdehyde Dehydrogenase From Ha... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ta4 | ||||||
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Title | Crystal Structure Of Aspartate-Semialdehyde Dehydrogenase From Haemophilus Influenzae with a Bound Arsenate | ||||||
Components | Aspartate-semialdehyde dehydrogenase | ||||||
Keywords | OXIDOREDUCTASE / Aspartate-Semialdehyde Dehydrogenase / Arsenate / Aspartate Biosynthetic Pathway / Haemophilus Influenzae | ||||||
Function / homology | Function and homology information aspartate-semialdehyde dehydrogenase / aspartate-semialdehyde dehydrogenase activity / 'de novo' L-methionine biosynthetic process / threonine biosynthetic process / diaminopimelate biosynthetic process / isoleucine biosynthetic process / lysine biosynthetic process via diaminopimelate / NAD binding / NADP binding / protein dimerization activity Similarity search - Function | ||||||
Biological species | Haemophilus influenzae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.28 Å | ||||||
Authors | Viola, R.E. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2004 Title: Structural basis for discrimination between oxyanion substrates or inhibitors in aspartate-beta-semialdehyde dehydrogenase. Authors: Faehnle, C.R. / Blanco, J. / Viola, R.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ta4.cif.gz | 81.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ta4.ent.gz | 61.6 KB | Display | PDB format |
PDBx/mmJSON format | 1ta4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ta4_validation.pdf.gz | 437.3 KB | Display | wwPDB validaton report |
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Full document | 1ta4_full_validation.pdf.gz | 445.2 KB | Display | |
Data in XML | 1ta4_validation.xml.gz | 16.2 KB | Display | |
Data in CIF | 1ta4_validation.cif.gz | 22 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ta/1ta4 ftp://data.pdbj.org/pub/pdb/validation_reports/ta/1ta4 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 40583.734 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Haemophilus influenzae (bacteria) / Gene: ASD, HI0646 / Production host: Escherichia coli (E. coli) References: UniProt: P44801, aspartate-semialdehyde dehydrogenase | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, ammonium acetate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.28→50 Å / Num. obs: 18047 / % possible obs: 95.2 % / Observed criterion σ(F): 3.9 / Observed criterion σ(I): 3.9 |
Reflection shell | Resolution: 2.28→2.37 Å / % possible all: 97.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.28→50 Å
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Refinement step | Cycle: LAST / Resolution: 2.28→50 Å
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