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- PDB-7seg: Crystal structure of the complex of CD16A bound by an anti-CD16A Fab -

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Basic information

Entry
Database: PDB / ID: 7seg
TitleCrystal structure of the complex of CD16A bound by an anti-CD16A Fab
Components
  • Low affinity immunoglobulin gamma Fc region receptor III-A
  • anti-CD16A Fab heavy chain
  • anti-CD16a Fab light chain
KeywordsANTITUMOR PROTEIN / antibody / fragment / CD16a / fab
Function / homology
Function and homology information


low-affinity IgG receptor activity / immune receptor activity / natural killer cell degranulation / IgG receptor activity / Fc-gamma receptor III complex / Fc-gamma receptor signaling pathway / macrophage activation / natural killer cell activation / antibody-dependent cellular cytotoxicity / IgG binding ...low-affinity IgG receptor activity / immune receptor activity / natural killer cell degranulation / IgG receptor activity / Fc-gamma receptor III complex / Fc-gamma receptor signaling pathway / macrophage activation / natural killer cell activation / antibody-dependent cellular cytotoxicity / IgG binding / natural killer cell mediated cytotoxicity / positive regulation of natural killer cell proliferation / FCGR activation / Role of phospholipids in phagocytosis / FCGR3A-mediated IL10 synthesis / FCGR3A-mediated phagocytosis / phosphatidylinositol 3-kinase/protein kinase B signal transduction / calcium-mediated signaling / Regulation of actin dynamics for phagocytic cup formation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / positive regulation of tumor necrosis factor production / cell surface receptor signaling pathway / immune response / external side of plasma membrane / extracellular space / extracellular exosome / plasma membrane
Similarity search - Function
: / Immunoglobulin domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Low affinity immunoglobulin gamma Fc region receptor III-A
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.156 Å
AuthorsKiefer, J.R. / Wallweber, H.A. / Polson, A.G.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Leukemia / Year: 2022
Title: A BCMA/CD16A bispecific innate cell engager for the treatment of multiple myeloma.
Authors: Kakiuchi-Kiyota, S. / Ross, T. / Wallweber, H.A. / Kiefer, J.R. / Schutten, M.M. / Adedeji, A.O. / Cai, H. / Hendricks, R. / Cohen, S. / Myneni, S. / Liu, L. / Fullerton, A. / Corr, N. / Yu, ...Authors: Kakiuchi-Kiyota, S. / Ross, T. / Wallweber, H.A. / Kiefer, J.R. / Schutten, M.M. / Adedeji, A.O. / Cai, H. / Hendricks, R. / Cohen, S. / Myneni, S. / Liu, L. / Fullerton, A. / Corr, N. / Yu, L. / de Almeida Nagata, D. / Zhong, S. / Leong, S.R. / Li, J. / Nakamura, R. / Sumiyoshi, T. / Li, J. / Ovacik, A.M. / Zheng, B. / Dillon, M. / Spiess, C. / Wingert, S. / Rajkovic, E. / Ellwanger, K. / Reusch, U. / Polson, A.G.
History
DepositionSep 30, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 24, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 26, 2022Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed ..._citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Apr 20, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Apr 3, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.4Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
L: anti-CD16a Fab light chain
H: anti-CD16A Fab heavy chain
B: anti-CD16a Fab light chain
A: anti-CD16A Fab heavy chain
D: Low affinity immunoglobulin gamma Fc region receptor III-A
C: Low affinity immunoglobulin gamma Fc region receptor III-A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)146,71711
Polymers145,5376
Non-polymers1,1805
Water3,567198
1
L: anti-CD16a Fab light chain
H: anti-CD16A Fab heavy chain
C: Low affinity immunoglobulin gamma Fc region receptor III-A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)73,3036
Polymers72,7683
Non-polymers5353
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: anti-CD16a Fab light chain
A: anti-CD16A Fab heavy chain
D: Low affinity immunoglobulin gamma Fc region receptor III-A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)73,4145
Polymers72,7683
Non-polymers6462
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)45.307, 220.560, 70.825
Angle α, β, γ (deg.)90.000, 108.550, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 1 types, 2 molecules DC

#3: Protein Low affinity immunoglobulin gamma Fc region receptor III-A / CD16a antigen / Fc-gamma RIII-alpha / Fc-gamma RIII / Fc-gamma RIIIa / FcRIII / FcRIIIa / FcR-10 / ...CD16a antigen / Fc-gamma RIII-alpha / Fc-gamma RIII / Fc-gamma RIIIa / FcRIII / FcRIIIa / FcR-10 / IgG Fc receptor III-2


Mass: 20456.789 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FCGR3A, CD16A, FCG3, FCGR3, IGFR3 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Tni Pro / References: UniProt: P08637

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Antibody , 2 types, 4 molecules LBHA

#1: Antibody anti-CD16a Fab light chain


Mass: 25662.602 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) / Strain (production host): 69F8
#2: Antibody anti-CD16A Fab heavy chain


Mass: 26649.020 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) / Strain (production host): 69F8

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Sugars , 2 types, 4 molecules

#4: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#6: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 199 molecules

#5: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 198 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.31 Å3/Da / Density % sol: 46.64 %
Crystal growTemperature: 292 K / Method: vapor diffusion, hanging drop / pH: 5.5
Details: 0.1M Bis-Tris pH5.5, 0.2M MgCl2, 0.01M Betaine HCl, 25% PEG3350

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Data collection

DiffractionMean temperature: 93 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.97946 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 24, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97946 Å / Relative weight: 1
Reflection twinOperator: -h,-k,h+l / Fraction: 0.33
ReflectionResolution: 2.15→35 Å / Num. obs: 64779 / % possible obs: 92.8 % / Redundancy: 2.9 % / Rmerge(I) obs: 0.098 / Rpim(I) all: 0.065 / Rrim(I) all: 0.119 / Χ2: 0.956 / Net I/σ(I): 6.1 / Num. measured all: 184828
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
2.15-2.191.70.55222090.4350.4790.7340.46264.1
2.19-2.231.80.58126200.4520.4790.7570.51275.6
2.23-2.2720.54629970.5090.4420.7070.52785
2.27-2.322.20.53429810.5460.4210.6840.50986.5
2.32-2.372.50.50733190.6010.3780.6360.47694.8
2.37-2.422.70.43933810.7220.3120.5410.51897
2.42-2.482.80.40233900.7660.2780.4910.53497.5
2.48-2.552.90.35733830.8210.2440.4340.52897.8
2.55-2.622.90.31133870.8530.2120.3780.58997
2.62-2.712.90.26833880.890.1830.3260.62996.8
2.71-2.812.80.21932630.9160.1520.2680.75492.8
2.81-2.9230.18933440.9360.1280.2290.82596.7
2.92-3.053.10.16533820.9470.1090.1980.88896.7
3.05-3.213.10.13533560.9640.090.1631.09796.2
3.21-3.413.20.11433260.9730.0750.1371.22796.3
3.41-3.683.10.09433090.9790.0630.1141.46593.8
3.68-4.043.40.08434090.9840.0530.11.42198.6
4.04-4.633.40.06934710.9880.0440.0821.43498.6
4.63-5.833.30.06433890.990.0410.0771.31596.4
5.83-353.40.06834750.9910.0440.0811.51398.2

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Processing

Software
NameVersionClassification
SCALEPACKdata scaling
PHENIX1.16_3549refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 14EJ

14ej


Resolution: 2.156→33.885 Å / Cross valid method: THROUGHOUT / σ(F): 75.31 / Phase error: 35.78 / Stereochemistry target values: TWIN_LSQ_F
RfactorNum. reflection% reflection
Rfree0.2339 3341 5.16 %
Rwork0.1894 61391 -
obs0.1938 64736 91.8 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 163.51 Å2 / Biso mean: 48.4284 Å2 / Biso min: 23.82 Å2
Refinement stepCycle: final / Resolution: 2.156→33.885 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9068 0 76 198 9342
Biso mean--59.3 38.62 -
Num. residues----1186
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 20

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.1638-2.20110.2875830.29321800188353
2.2011-2.24110.28771440.28162550269473
2.2411-2.28410.3051450.27332905305082
2.2841-2.33070.31551430.27642834297783
2.3307-2.38120.28911910.27763168335989
2.3812-2.43650.24481750.26873174334992
2.4365-2.49740.26441820.25933201338392
2.4974-2.56470.27611650.24913231339693
2.5647-2.640.32041630.25413252341592
2.64-2.7250.32071730.24843210338392
2.725-2.82210.29051480.24273131327989
2.8221-2.93480.28751790.23853129330891
2.9348-3.06790.26281680.23063232340092
3.0679-3.22890.27031700.21463166333691
3.2289-3.43020.27951360.19763197333392
3.4302-3.69350.2161740.18043145331989
3.6935-4.06210.21381600.16223228338893
4.0621-4.64310.15831480.12983306345494
4.6431-5.82410.21381500.13683246339692
5.8241-19.57250.19541670.1573286345393
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.01581.82641.32792.9481.05170.6657-0.0195-0.05120.11140.1133-0.16940.40380.0956-0.25750.00170.24680.0203-0.00050.6367-0.06940.4083-4.66416.135141.57
22.0196-0.39330.46182.5907-0.02251.2027-0.04840.0066-0.07390.5628-0.0147-0.07720.2666-0.0904-0.00010.4084-0.0177-0.02960.5842-0.01770.31314.885-18.001135.885
30.74850.533-0.11871.30820.70920.8086-0.00230.0532-0.0635-0.017-0.0739-0.2640.04970.1169-00.2120.0387-0.02290.5549-0.03360.312818.91919.801142.692
40.8387-0.01960.32621.5584-0.35731.2848-0.0594-0.0443-0.18320.72270.0983-0.4189-0.0311-0.21440.02390.22790.02510.01980.5831-0.04810.319313.99122.89150.664
50.6851-0.38040.07061.37070.99550.95890.04440.0461-0.15080.5037-0.1207-0.1638-0.15280.085200.33310.0094-0.08210.5171-0.10380.366720.72118.086151.271
60.50570.31430.21340.493-0.22160.5033-0.05670.2066-0.02920.0124-0.04250.1441-0.1316-0.0134-0.00010.25490.02-0.05870.4557-0.02180.254111.9620.776142.893
70.79820.5379-0.75961.35440.25781.2779-0.06880.3446-0.04470.1610.1267-0.11330.24310.1813-0.00030.2530.033-0.02520.5064-0.03140.33915.814-7.957131.78
80.7563-0.2089-0.19441.331-0.84121.0770.06020.1776-0.4137-0.08390.064-0.37360.3315-0.10990.00040.23690.013-0.02330.5848-0.07890.371616.386-9.664123.605
90.3615-0.917-0.38582.70941.19550.601-0.1529-0.53690.3243-0.7818-0.16880.7022-0.3545-0.0165-0.28910.13130.0678-0.00480.7622-0.05920.5168-8.054-11.20397.36
10-0.01320.0121-0.0110.14590.12340.04580.26650.4642-0.2761-0.3487-0.6370.69260.0682-0.682-0.00170.43660.0605-0.1340.713-0.11960.4299-9.128-18.93390.852
111.5362-0.5605-0.77131.9911-0.80491.22690.0094-0.18520.0837-0.0549-0.03430.54140.1537-0.36160.00010.28060.0036-0.05740.5255-0.0440.4337-3.933-21.279101.79
120.3272-0.1875-0.46840.63990.16210.50740.0645-0.21430.19610.17390.0510.15230.38630.08140.00450.2451-0.0237-0.00810.6835-0.10290.3732-2.715-14.861102.912
130.8491-0.01150.32570.32720.02580.06680.11380.1119-0.1428-0.5322-0.52260.3899-0.2787-0.7782-0.01170.3091-0.0226-0.01940.5414-0.09210.3844-3.044-9.06198.794
142.5578-0.4403-0.05592.30140.56921.2234-0.0450.2730.2716-0.33240.1401-0.1172-0.2646-0.12060.00030.382-0.01130.00040.6067-0.01590.35195.24617.919105.731
152.01920.10510.24362.13560.70340.428-0.0116-0.0157-0.0253-0.2460.0518-0.18460.00990.0212-00.31320.0090.01340.486-0.02850.279318.391-19.8694.052
160.1426-0.3626-0.02710.7372-0.2020.18870.10420.0582-0.0598-0.09970.1369-0.17250.11340.1350.00790.2903-0.03010.05560.4947-0.00120.335310.269-23.68198.812
170.1993-0.03610.08230.1930.30250.4521-0.15770.14340.2122-0.18930.1507-0.4266-0.1580.2105-0.00630.23220.02910.03810.5428-0.04030.454523.683-0.75698.693
180.07490.34620.05660.9178-0.08051.7318-0.0716-0.1420.0336-0.15430.0657-0.0515-0.48650.32150.00890.2659-0.02990.01880.5324-0.03880.398415.2669.729113.143
191.18450.26910.9392.6201-1.14741.4418-0.1141-0.12020.02920.17870.1094-0.0941-0.2382-0.05460.00010.29-0.0036-0.01620.5072-0.06360.323515.0148.251114.999
200.3787-0.03140.35761.2562-0.60310.6151-0.1127-0.41350.2756-0.12850.07450.0827-0.08670.011-0.00010.42380.0608-0.06280.7088-0.04170.402111.63-46.79998.864
210.43740.26240.08860.8002-0.77590.9776-0.0395-1.18250.17960.431-0.07360.5866-0.75860.35020.01160.4804-0.00870.00270.957-0.00950.580812.855-49.13112.481
220.1939-0.23-0.10250.40730.11140.2745-0.0143-0.7546-0.51070.0356-0.1158-0.02010.06810.46910.00030.37820.068-0.03210.6635-0.01060.475517.773-53.492106.296
230.37940.24780.10860.5586-0.12450.1336-0.2046-0.6402-0.41840.5620.42320.30370.53490.262-0.00090.56420.1260.02140.70260.02260.50468.542-53.913108.28
240.1861-0.20580.19510.1869-0.17030.1436-0.1067-0.0842-0.15880.1334-0.2011-0.24060.18520.40950.00060.39450.0696-0.07880.6509-0.03430.455913.658-50.52489.06
250.17270.1165-0.08840.0904-0.05050.02890.4279-0.454-0.5999-0.1746-0.4110.3523-0.0292-0.45060.00330.5295-0.049-0.06620.71770.15360.6231-2.348-42.46492.281
260.0697-0.1536-0.06010.6399-0.12450.25130.51440.3658-0.3842-0.5713-0.2288-0.0393-0.21480.54690.00030.6251-0.0155-0.03460.6329-0.05940.376914.077-41.52382.519
270.4773-0.54840.37720.656-0.330.60330.18240.3216-0.6149-0.1689-0.17890.1929-0.12970.16710.00190.49540.034-0.08060.7385-0.09730.37375.092-36.13581.78
280.1608-0.20850.10110.95860.19410.20850.3699-0.01440.1356-0.24630.0027-0.4590.26860.42210.00020.65640.1119-0.10990.7148-0.12870.370513.4-45.81977.511
290.00820.0741-0.04381.7419-1.15770.75090.1352-0.078-0.09340.6064-0.05390.89280.1393-0.75050.1120.8316-0.0305-0.29990.5612-0.2780.689-4.401-46.46287.531
300.5655-0.0607-0.2445-0.0435-0.14620.35590.37540.11030.7066-0.1611-0.2526-0.13220.3553-0.5787-0.00130.4202-0.00980.00120.73740.01590.416211.81347.264142.196
310.56730.0098-0.25680.3364-0.0220.0737-0.54080.6729-0.0912-0.73250.28360.8284-0.3541-0.2127-0.01130.8263-0.0657-0.18050.80020.13540.495413.4350.026129.734
320.22010.1597-0.02320.097-0.05810.0925-0.31490.102-0.0803-0.5015-0.38530.3125-0.45150.275-0.01680.6626-0.0014-0.04180.85180.03660.458318.79354.438136.903
330.35510.4026-0.47760.2882-0.28990.4672-0.17760.29560.387-0.02610.17990.233-0.7885-0.62440.00310.4698-0.0109-0.09220.78230.08430.439410.87152.346141.147
340.1881-0.12630.14560.072-0.07890.0486-0.0584-0.01140.6684-0.4722-0.35160.4322-0.3716-0.849800.4780.1145-0.0380.7627-0.15260.5875-1.76542.438148.819
350.39410.7921-0.43241.82-0.17090.94840.0377-0.4623-0.05450.1994-0.156-0.0021-0.1509-0.0052-0.00140.3734-0.0288-0.00350.6535-0.11380.32599.05738.674159.023
360.63840.6198-0.64492.1456-0.48970.6920.0521-0.31690.43910.1334-0.364-0.0095-0.2733-0.2411-0.00040.48270.02880.02810.8004-0.14910.4297.29746.045160.366
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN L AND RESID 1:112 )L1 - 112
2X-RAY DIFFRACTION2( CHAIN L AND RESID 113:211 )L113 - 211
3X-RAY DIFFRACTION3( CHAIN H AND RESID 5:44 )H5 - 44
4X-RAY DIFFRACTION4( CHAIN H AND RESID 45:63 )H45 - 63
5X-RAY DIFFRACTION5( CHAIN H AND RESID 64:91 )H64 - 91
6X-RAY DIFFRACTION6( CHAIN H AND RESID 92:118 )H92 - 118
7X-RAY DIFFRACTION7( CHAIN H AND RESID 119:182 )H119 - 182
8X-RAY DIFFRACTION8( CHAIN H AND RESID 183:213 )H183 - 213
9X-RAY DIFFRACTION9( CHAIN B AND RESID 1:17 )B1 - 17
10X-RAY DIFFRACTION10( CHAIN B AND RESID 18:29 )B18 - 29
11X-RAY DIFFRACTION11( CHAIN B AND RESID 30:74 )B30 - 74
12X-RAY DIFFRACTION12( CHAIN B AND RESID 75:90 )B75 - 90
13X-RAY DIFFRACTION13( CHAIN B AND RESID 91:112 )B91 - 112
14X-RAY DIFFRACTION14( CHAIN B AND RESID 113:211 )B113 - 211
15X-RAY DIFFRACTION15( CHAIN A AND RESID 5:91 )A5 - 91
16X-RAY DIFFRACTION16( CHAIN A AND RESID 92:113 )A92 - 113
17X-RAY DIFFRACTION17( CHAIN A AND RESID 114:126 )A114 - 126
18X-RAY DIFFRACTION18( CHAIN A AND RESID 127:164 )A127 - 164
19X-RAY DIFFRACTION19( CHAIN A AND RESID 165:213 )A165 - 213
20X-RAY DIFFRACTION20( CHAIN D AND RESID 3:21 )D3 - 21
21X-RAY DIFFRACTION21( CHAIN D AND RESID 22:41 )D22 - 41
22X-RAY DIFFRACTION22( CHAIN D AND RESID 42:59 )D42 - 59
23X-RAY DIFFRACTION23( CHAIN D AND RESID 60:78 )D60 - 78
24X-RAY DIFFRACTION24( CHAIN D AND RESID 79:92 )D79 - 92
25X-RAY DIFFRACTION25( CHAIN D AND RESID 93:102 )D93 - 102
26X-RAY DIFFRACTION26( CHAIN D AND RESID 103:124 )D103 - 124
27X-RAY DIFFRACTION27( CHAIN D AND RESID 125:146 )D125 - 146
28X-RAY DIFFRACTION28( CHAIN D AND RESID 147:164 )D147 - 164
29X-RAY DIFFRACTION29( CHAIN D AND RESID 165:172 )D165 - 172
30X-RAY DIFFRACTION30( CHAIN C AND RESID 2:21 )C2 - 21
31X-RAY DIFFRACTION31( CHAIN C AND RESID 22:41 )C22 - 41
32X-RAY DIFFRACTION32( CHAIN C AND RESID 42:63 )C42 - 63
33X-RAY DIFFRACTION33( CHAIN C AND RESID 64:91 )C64 - 91
34X-RAY DIFFRACTION34( CHAIN C AND RESID 92:102 )C92 - 102
35X-RAY DIFFRACTION35( CHAIN C AND RESID 103:146 )C103 - 146
36X-RAY DIFFRACTION36( CHAIN C AND RESID 147:172 )C147 - 172

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