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- PDB-7oau: Nanobody C5 bound to Kent variant RBD (N501Y) -

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Basic information

Entry
Database: PDB / ID: 7oau
TitleNanobody C5 bound to Kent variant RBD (N501Y)
Components
  • C5
  • Spike protein S1
KeywordsANTIVIRAL PROTEIN / RBD / nanobody / high affinity
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
Biological speciesSevere acute respiratory syndrome coronavirus 2
Lama glama (llama)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.65 Å
AuthorsNaismith, J.H. / Mikolajek, H.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
Wellcome Trust100209/Z/12/Z) United Kingdom
Engineering and Physical Sciences Research CouncilEP/S025243/1 United Kingdom
Citation
Journal: Nat Commun / Year: 2021
Title: A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19.
Authors: Jiandong Huo / Halina Mikolajek / Audrey Le Bas / Jordan J Clark / Parul Sharma / Anja Kipar / Joshua Dormon / Chelsea Norman / Miriam Weckener / Daniel K Clare / Peter J Harrison / Julia A ...Authors: Jiandong Huo / Halina Mikolajek / Audrey Le Bas / Jordan J Clark / Parul Sharma / Anja Kipar / Joshua Dormon / Chelsea Norman / Miriam Weckener / Daniel K Clare / Peter J Harrison / Julia A Tree / Karen R Buttigieg / Francisco J Salguero / Robert Watson / Daniel Knott / Oliver Carnell / Didier Ngabo / Michael J Elmore / Susan Fotheringham / Adam Harding / Lucile Moynié / Philip N Ward / Maud Dumoux / Tessa Prince / Yper Hall / Julian A Hiscox / Andrew Owen / William James / Miles W Carroll / James P Stewart / James H Naismith / Raymond J Owens /
Abstract: SARS-CoV-2 remains a global threat to human health particularly as escape mutants emerge. There is an unmet need for effective treatments against COVID-19 for which neutralizing single domain ...SARS-CoV-2 remains a global threat to human health particularly as escape mutants emerge. There is an unmet need for effective treatments against COVID-19 for which neutralizing single domain antibodies (nanobodies) have significant potential. Their small size and stability mean that nanobodies are compatible with respiratory administration. We report four nanobodies (C5, H3, C1, F2) engineered as homotrimers with pmolar affinity for the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. Crystal structures show C5 and H3 overlap the ACE2 epitope, whilst C1 and F2 bind to a different epitope. Cryo Electron Microscopy shows C5 binding results in an all down arrangement of the Spike protein. C1, H3 and C5 all neutralize the Victoria strain, and the highly transmissible Alpha (B.1.1.7 first identified in Kent, UK) strain and C1 also neutralizes the Beta (B.1.35, first identified in South Africa). Administration of C5-trimer via the respiratory route showed potent therapeutic efficacy in the Syrian hamster model of COVID-19 and separately, effective prophylaxis. The molecule was similarly potent by intraperitoneal injection.
#1: Journal: Res Sq / Year: 2021
Title: A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19
Authors: Huo, J. / Mikolajek, H. / Le Bas, A. / Clark, J. / Sharma, P. / Kipar, A. / Dormon, J. / Norman, C. / Weckener, M. / Clare, D. / Harrison, P. / Tree, J. / Buttigieg, K. / Salguero, F. / ...Authors: Huo, J. / Mikolajek, H. / Le Bas, A. / Clark, J. / Sharma, P. / Kipar, A. / Dormon, J. / Norman, C. / Weckener, M. / Clare, D. / Harrison, P. / Tree, J. / Buttigieg, K. / Salguero, F. / Watson, R. / Knott, D. / Carnell, O. / Ngabo, D. / Elmore, M. / Fotheringham, S. / Harding, A. / Ward, P. / Moynie, L. / Dumoux, M. / Hall, Y. / Hiscox, J. / Owen, A. / James, W. / Carroll, M. / Stewart, J. / Naismith, J. / Owens, R.
History
DepositionApr 20, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 11, 2021Provider: repository / Type: Initial release
Revision 2.0Oct 6, 2021Group: Atomic model / Data collection / Database references
Category: atom_site / citation ...atom_site / citation / citation_author / database_PDB_rev / database_PDB_rev_record / diffrn_source / pdbx_database_proc
Item: _atom_site.pdbx_tls_group_id / _diffrn_source.pdbx_synchrotron_site
Revision 2.1Jan 31, 2024Group: Data collection / Derived calculations / Refinement description
Category: atom_type / chem_comp_atom ...atom_type / chem_comp_atom / chem_comp_bond / diffrn_source / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _atom_type.pdbx_N_electrons / _atom_type.pdbx_scat_Z ..._atom_type.pdbx_N_electrons / _atom_type.pdbx_scat_Z / _diffrn_source.pdbx_synchrotron_site / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
AAA: Spike protein S1
BBB: C5
EEE: Spike protein S1
FFF: C5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)75,8817
Polymers75,3464
Non-polymers5353
Water8,467470
1
AAA: Spike protein S1
BBB: C5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,9864
Polymers37,6732
Non-polymers3132
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2010 Å2
ΔGint-3 kcal/mol
Surface area15000 Å2
MethodPISA
2
EEE: Spike protein S1
FFF: C5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,8943
Polymers37,6732
Non-polymers2211
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1680 Å2
ΔGint-3 kcal/mol
Surface area15160 Å2
MethodPISA
Unit cell
Length a, b, c (Å)28.810, 153.730, 75.850
Angle α, β, γ (deg.)90.000, 100.330, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11AAA
21EEE
32BBB
42FFF

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111ASNASNPROPROAAAA334 - 5275 - 198
211ASNASNPROPROEEEC334 - 5275 - 198
322GLNGLNSERSERBBBB1 - 1203 - 122
422GLNGLNSERSERFFFD1 - 1203 - 122

NCS ensembles :
IDDetails
1Local NCS retraints between domains: 1 2
2Local NCS retraints between domains: 3 4

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Components

#1: Protein Spike protein S1


Mass: 23765.652 Da / Num. of mol.: 2 / Mutation: N501Y
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#2: Antibody C5


Mass: 13907.522 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli (E. coli)
#3: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#4: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 470 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.19 Å3/Da / Density % sol: 43.92 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M Ammonium fluoride and 20 % Peg 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.9999 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 21, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9999 Å / Relative weight: 1
ReflectionResolution: 1.65→39 Å / Num. obs: 77705 / % possible obs: 100 % / Redundancy: 6.6 % / CC1/2: 1 / Rpim(I) all: 0.044 / Net I/σ(I): 10
Reflection shellResolution: 1.65→1.69 Å / Redundancy: 6 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 5819 / CC1/2: 0.6 / Rpim(I) all: 0.56 / % possible all: 100

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Processing

Software
NameVersionClassification
REFMAC5.8.0267refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7OAO

7oao


Resolution: 1.65→38.462 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.957 / SU B: 4.776 / SU ML: 0.078 / Cross valid method: FREE R-VALUE / ESU R: 0.096 / ESU R Free: 0.091
Details: Hydrogens have been added in their riding positions
RfactorNum. reflection% reflection
Rfree0.1992 3776 4.861 %
Rwork0.1754 73903 -
all0.177 --
obs-77679 99.967 %
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.3 Å / Solvent model: MASK BULK SOLVENT
Displacement parametersBiso mean: 29.12 Å2
Baniso -1Baniso -2Baniso -3
1-0.02 Å20 Å20.707 Å2
2---2.163 Å20 Å2
3---1.768 Å2
Refinement stepCycle: LAST / Resolution: 1.65→38.462 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4892 0 34 470 5396
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0070.0125183
X-RAY DIFFRACTIONr_bond_other_d0.0010.0184667
X-RAY DIFFRACTIONr_angle_refined_deg1.3371.6547089
X-RAY DIFFRACTIONr_angle_other_deg1.3231.58110739
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.9135664
X-RAY DIFFRACTIONr_dihedral_angle_2_deg31.74922.052268
X-RAY DIFFRACTIONr_dihedral_angle_3_deg11.5615775
X-RAY DIFFRACTIONr_dihedral_angle_4_deg16.2871530
X-RAY DIFFRACTIONr_chiral_restr0.060.2670
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.026048
X-RAY DIFFRACTIONr_gen_planes_other0.0010.021328
X-RAY DIFFRACTIONr_nbd_refined0.180.2790
X-RAY DIFFRACTIONr_symmetry_nbd_other0.1730.24327
X-RAY DIFFRACTIONr_nbtor_refined0.1680.22510
X-RAY DIFFRACTIONr_symmetry_nbtor_other0.0730.22576
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1520.2353
X-RAY DIFFRACTIONr_symmetry_nbd_refined0.1590.223
X-RAY DIFFRACTIONr_nbd_other0.1590.292
X-RAY DIFFRACTIONr_symmetry_xyhbond_nbd_refined0.1360.241
X-RAY DIFFRACTIONr_mcbond_it1.3141.6582564
X-RAY DIFFRACTIONr_mcbond_other1.3141.6572563
X-RAY DIFFRACTIONr_mcangle_it1.8932.483210
X-RAY DIFFRACTIONr_mcangle_other1.8922.4813211
X-RAY DIFFRACTIONr_scbond_it2.1791.9472619
X-RAY DIFFRACTIONr_scbond_other2.1791.9482620
X-RAY DIFFRACTIONr_scangle_it3.3562.8233862
X-RAY DIFFRACTIONr_scangle_other3.3552.8243863
X-RAY DIFFRACTIONr_lrange_it5.96320.6025680
X-RAY DIFFRACTIONr_lrange_other5.96120.5795678
X-RAY DIFFRACTIONr_ncsr_local_group_10.0560.056303
X-RAY DIFFRACTIONr_ncsr_local_group_20.0640.053667
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)Weight position
11AAAX-RAY DIFFRACTIONLocal ncs0.055970.0501
12EEEX-RAY DIFFRACTIONLocal ncs0.055970.0501
23BBBX-RAY DIFFRACTIONLocal ncs0.064150.0501
24FFFX-RAY DIFFRACTIONLocal ncs0.064150.0501
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.65-1.6930.3292740.3165545X-RAY DIFFRACTION99.9485
1.693-1.7390.2872890.2865182X-RAY DIFFRACTION99.9817
1.739-1.790.2792860.265189X-RAY DIFFRACTION99.9817
1.79-1.8450.2272420.2295015X-RAY DIFFRACTION100
1.845-1.9050.2072420.2054908X-RAY DIFFRACTION99.9806
1.905-1.9720.2162200.1854674X-RAY DIFFRACTION100
1.972-2.0460.2062180.1744631X-RAY DIFFRACTION99.9794
2.046-2.130.1792190.1694310X-RAY DIFFRACTION100
2.13-2.2240.2022110.1594282X-RAY DIFFRACTION99.9778
2.224-2.3330.1841950.1533939X-RAY DIFFRACTION99.9758
2.333-2.4590.2041730.1593863X-RAY DIFFRACTION100
2.459-2.6080.182290.1613615X-RAY DIFFRACTION99.974
2.608-2.7880.1911560.1523364X-RAY DIFFRACTION100
2.788-3.0110.1891600.1583157X-RAY DIFFRACTION100
3.011-3.2980.1871510.1732934X-RAY DIFFRACTION100
3.298-3.6860.2011350.1712623X-RAY DIFFRACTION99.9275
3.686-4.2550.1921270.1582321X-RAY DIFFRACTION99.9184
4.255-5.2070.1491170.1481942X-RAY DIFFRACTION99.8061
5.207-7.3470.224850.1971547X-RAY DIFFRACTION99.9388
7.347-38.4620.239470.196837X-RAY DIFFRACTION99.2144
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.84530.05061.218518.92043.46162.3672-0.0263-0.0179-0.00240.42180.0568-0.27440.0210.0001-0.03050.23110.0112-0.00780.1540.0140.136111.423-1.139-25.53
21.04411.0786-0.24026.5546-2.19661.87680.0389-0.1718-0.15410.5111-0.0841-0.44010.15250.06490.04520.17370.0161-0.03760.08820.00310.086213.0598.775-31.466
32.3148-1.3651-0.75984.9801-0.00780.9402-0.19530.0388-0.21270.39550.0590.54090.2448-0.10010.13630.229-0.05050.05390.1439-0.01430.28281.474-1.572-35.579
41.48881.0192-0.52213.1578-1.0981.3008-0.0320.0251-0.102-0.00750.03950.12920.0594-0.0802-0.00750.02620.00750.00280.0566-0.0150.02738.51121.349-40.127
53.9086-4.4325-3.72296.59543.58696.2282-0.1684-0.06460.0129-0.0008-0.0694-0.06290.14-0.07680.23770.1586-0.0176-0.01940.0837-0.00270.204412.978-6.982-39.52
62.283-0.60840.30311.77540.18550.11360.06850.11280.08050.052-0.08060.147-0.0231-0.01850.01210.1320.00220.03510.09130.00030.06610.14445.478-36.332
70.8369-1.30730.31839.8954-0.94450.3504-0.022-0.0350.15740.01450.0138-0.2263-0.01940.0380.00820.1221-0.00530.02430.093-0.02050.08497.72254.344-31.47
80.9918-1.1759-0.20732.46880.71860.4421-0.0059-0.12530.05230.230.0248-0.0079-0.02730.0063-0.0190.1689-0.02260.03340.1035-0.02790.03594.25346.555-29.154
96.0567-4.1822-4.00924.21132.7643.83260.0859-0.1260.1302-0.0575-0.0674-0.0041-0.14010.0288-0.01850.1133-0.02390.04170.0467-0.02070.07671.23154.51-35.253
102.7966-4.1621.31311.4563-1.47963.5881-0.1648-0.1810.09870.39070.16710.4051-0.1941-0.1982-0.00230.1550.00920.06680.145-0.0360.232911.317-11.64712.44
110.6999-0.14390.21133.54910.09070.5483-0.0250.00660.29240.1433-0.0501-0.0614-0.15320.07030.07510.0996-0.01030.00210.070.0090.154515.54-8.0522.956
121.71060.94640.68642.54590.8711.3626-0.05440.14790.1167-0.15690.05490.1198-0.03860.0858-0.00050.028-0.0020.00460.03840.01050.022514.154-23.729-2.838
132.90462.6612.13383.05242.27932.68160.08720.1592-0.11850.08570.0643-0.12270.15620.1011-0.15150.04720.02550.00270.06660.00040.013511.776-36.735-3.627
141.699-3.51081.06998.1834-1.88340.78970.05890.0279-0.0099-0.2043-0.12140.19420.0144-0.00190.06250.14950.00010.00260.08620.0080.293310.044-3.163-0.407
152.5359-0.4957-0.51593.56-0.05840.11590.10940.0845-0.26350.0739-0.1632-0.3146-0.0105-0.00460.05380.1723-0.0008-0.05850.1004-0.0230.125721.628-52.1160.133
162.08490.2817-0.494112.98584.37753.5387-0.1084-0.0529-0.1930.2333-0.02560.4357-0.0763-0.12980.1340.1291-0.0002-0.02140.08530.02010.109314.863-64.6614.929
171.4621-1.40510.09744.0083-0.2780.15610.0523-0.1837-0.1720.492-0.0220.00390.0904-0.0229-0.03030.2962-0.0565-0.07220.15070.03450.096916.825-52.8286.03
1811.373-10.017.390414.1367-5.93438.4650.13240.3381-0.18920.2501-0.4261-0.61790.16510.64470.29370.1171-0.0013-0.00980.19090.15420.3426.417-64.1893.287
Refinement TLS group
IDRefine-IDRefine TLS-IDSelectionAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1ALLAAA334 - 340
2X-RAY DIFFRACTION2ALLAAA341 - 362
3X-RAY DIFFRACTION3ALLAAA363 - 395
4X-RAY DIFFRACTION4ALLAAA396 - 513
5X-RAY DIFFRACTION5ALLAAA514 - 527
6X-RAY DIFFRACTION6ALLBBB1 - 36
7X-RAY DIFFRACTION7ALLBBB37 - 52
8X-RAY DIFFRACTION8ALLBBB53 - 106
9X-RAY DIFFRACTION9ALLBBB107 - 122
10X-RAY DIFFRACTION10ALLEEE334 - 346
11X-RAY DIFFRACTION11ALLEEE347 - 395
12X-RAY DIFFRACTION12ALLEEE396 - 470
13X-RAY DIFFRACTION13ALLEEE471 - 509
14X-RAY DIFFRACTION14ALLEEE510 - 527
15X-RAY DIFFRACTION15ALLFFF1 - 37
16X-RAY DIFFRACTION16ALLFFF38 - 49
17X-RAY DIFFRACTION17ALLFFF50 - 111
18X-RAY DIFFRACTION18ALLFFF112 - 121

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