+Open data
-Basic information
Entry | Database: PDB / ID: 7mrs | ||||||
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Title | Zebrafish CNTN4 APPb complex | ||||||
Components |
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Keywords | CELL ADHESION / Ig superfamily / amyloid precursor protein | ||||||
Function / homology | Function and homology information convergent extension / regulation of Notch signaling pathway / cell-cell adhesion mediator activity / vasculature development / axon extension / regulation of neuron differentiation / Golgi-associated vesicle / transition metal ion binding / axonogenesis / central nervous system development ...convergent extension / regulation of Notch signaling pathway / cell-cell adhesion mediator activity / vasculature development / axon extension / regulation of neuron differentiation / Golgi-associated vesicle / transition metal ion binding / axonogenesis / central nervous system development / axon guidance / brain development / cell-cell adhesion / neuron projection development / heparin binding / membrane => GO:0016020 / axon / plasma membrane Similarity search - Function | ||||||
Biological species | Danio rerio (zebrafish) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.93 Å | ||||||
Authors | Bouyain, S. / Karuppan, S.J. | ||||||
Funding support | United States, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2021 Title: Members of the vertebrate contactin and amyloid precursor protein families interact through a conserved interface. Authors: Karuppan, S.J. / Vogt, A. / Fischer, Z. / Ladutska, A. / Swiastyn, J. / McGraw, H.F. / Bouyain, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7mrs.cif.gz | 180.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7mrs.ent.gz | 125.3 KB | Display | PDB format |
PDBx/mmJSON format | 7mrs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7mrs_validation.pdf.gz | 436 KB | Display | wwPDB validaton report |
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Full document | 7mrs_full_validation.pdf.gz | 438.9 KB | Display | |
Data in XML | 7mrs_validation.xml.gz | 14.6 KB | Display | |
Data in CIF | 7mrs_validation.cif.gz | 19.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mr/7mrs ftp://data.pdbj.org/pub/pdb/validation_reports/mr/7mrs | HTTPS FTP |
-Related structure data
Related structure data | 7mqyC 7mrkC 7mrmC 7mrnC 7mroC 7mrqC 3ktmS 5e4sS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 33217.207 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Gene: cntn4, zgc:153573 / Plasmid: pET28 / Production host: Escherichia coli (E. coli) / References: UniProt: Q08C52 |
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#2: Protein | Mass: 21126.209 Da / Num. of mol.: 1 / Fragment: E1 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Gene: appb / Plasmid: pET32 / Production host: Escherichia coli (E. coli) / References: UniProt: B0CM02 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.96 % |
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Crystal grow | Temperature: 277 K / Method: batch mode / Details: 75 mM NaCl, 10 mM Na-HEPES pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Feb 10, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.93→89.77 Å / Num. obs: 13383 / % possible obs: 100 % / Redundancy: 10.4 % / Biso Wilson estimate: 58.35 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.165 / Rpim(I) all: 0.053 / Rrim(I) all: 0.174 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 2.93→3.1 Å / Redundancy: 10 % / Rmerge(I) obs: 1.163 / Mean I/σ(I) obs: 2 / Num. unique obs: 2111 / CC1/2: 0.697 / Rpim(I) all: 0.384 / Rrim(I) all: 1.227 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3KTM, 5E4S Resolution: 2.93→44.09 Å / SU ML: 0.3882 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 32.1231 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 71.82 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.93→44.09 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION
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