[English] 日本語
Yorodumi
- PDB-7mrs: Zebrafish CNTN4 APPb complex -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 7mrs
TitleZebrafish CNTN4 APPb complex
Components
  • Amyloid-beta A4 protein
  • Contactin-4
KeywordsCELL ADHESION / Ig superfamily / amyloid precursor protein
Function / homology
Function and homology information


convergent extension / regulation of Notch signaling pathway / cell-cell adhesion mediator activity / vasculature development / axon extension / regulation of neuron differentiation / Golgi-associated vesicle / transition metal ion binding / axonogenesis / central nervous system development ...convergent extension / regulation of Notch signaling pathway / cell-cell adhesion mediator activity / vasculature development / axon extension / regulation of neuron differentiation / Golgi-associated vesicle / transition metal ion binding / axonogenesis / central nervous system development / axon guidance / brain development / cell-cell adhesion / neuron projection development / heparin binding / membrane => GO:0016020 / axon / plasma membrane
Similarity search - Function
Amyloid-beta precursor protein / Amyloidogenic glycoprotein, copper-binding / Amyloidogenic glycoprotein, copper-binding domain conserved site / Amyloidogenic glycoprotein, copper-binding domain superfamily / Copper-binding of amyloid precursor, CuBD / Amyloid precursor protein (APP) copper-binding (CuBD) domain signature. / Amyloidogenic glycoprotein, amyloid-beta peptide superfamily / Beta-amyloid peptide (beta-APP) / Amyloidogenic glycoprotein, amyloid-beta peptide / Amyloid precursor protein (APP) E1 domain profile. ...Amyloid-beta precursor protein / Amyloidogenic glycoprotein, copper-binding / Amyloidogenic glycoprotein, copper-binding domain conserved site / Amyloidogenic glycoprotein, copper-binding domain superfamily / Copper-binding of amyloid precursor, CuBD / Amyloid precursor protein (APP) copper-binding (CuBD) domain signature. / Amyloidogenic glycoprotein, amyloid-beta peptide superfamily / Beta-amyloid peptide (beta-APP) / Amyloidogenic glycoprotein, amyloid-beta peptide / Amyloid precursor protein (APP) E1 domain profile. / Amyloid precursor protein (APP) E2 domain profile. / Amyloidogenic glycoprotein, extracellular / Amyloidogenic glycoprotein, heparin-binding / Amyloidogenic glycoprotein, E2 domain / E2 domain superfamily / Amyloidogenic glycoprotein, heparin-binding domain superfamily / Amyloid A4 N-terminal heparin-binding / E2 domain of amyloid precursor protein / amyloid A4 / Amyloidogenic glycoprotein / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Amyloid-beta A4 protein / Contactin-4 precursor
Similarity search - Component
Biological speciesDanio rerio (zebrafish)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.93 Å
AuthorsBouyain, S. / Karuppan, S.J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)1R15NS108371-01 United States
CitationJournal: J.Biol.Chem. / Year: 2021
Title: Members of the vertebrate contactin and amyloid precursor protein families interact through a conserved interface.
Authors: Karuppan, S.J. / Vogt, A. / Fischer, Z. / Ladutska, A. / Swiastyn, J. / McGraw, H.F. / Bouyain, S.
History
DepositionMay 9, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 5, 2022Provider: repository / Type: Initial release
Revision 1.1Feb 9, 2022Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: Contactin-4
B: Amyloid-beta A4 protein


Theoretical massNumber of molelcules
Total (without water)54,3432
Polymers54,3432
Non-polymers00
Water30617
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: surface plasmon resonance
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1210 Å2
ΔGint-8 kcal/mol
Surface area19280 Å2
MethodPISA
Unit cell
Length a, b, c (Å)74.980, 88.190, 89.770
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

-
Components

#1: Protein Contactin-4 / Contactin 4


Mass: 33217.207 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Danio rerio (zebrafish) / Gene: cntn4, zgc:153573 / Plasmid: pET28 / Production host: Escherichia coli (E. coli) / References: UniProt: Q08C52
#2: Protein Amyloid-beta A4 protein


Mass: 21126.209 Da / Num. of mol.: 1 / Fragment: E1 domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Danio rerio (zebrafish) / Gene: appb / Plasmid: pET32 / Production host: Escherichia coli (E. coli) / References: UniProt: B0CM02
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 17 / Source method: isolated from a natural source / Formula: H2O

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 2.73 Å3/Da / Density % sol: 54.96 %
Crystal growTemperature: 277 K / Method: batch mode / Details: 75 mM NaCl, 10 mM Na-HEPES pH 7.5

-
Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: RAYONIX MX300-HS / Detector: CCD / Date: Feb 10, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.93→89.77 Å / Num. obs: 13383 / % possible obs: 100 % / Redundancy: 10.4 % / Biso Wilson estimate: 58.35 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.165 / Rpim(I) all: 0.053 / Rrim(I) all: 0.174 / Net I/σ(I): 9.8
Reflection shellResolution: 2.93→3.1 Å / Redundancy: 10 % / Rmerge(I) obs: 1.163 / Mean I/σ(I) obs: 2 / Num. unique obs: 2111 / CC1/2: 0.697 / Rpim(I) all: 0.384 / Rrim(I) all: 1.227 / % possible all: 100

-
Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
MOSFLMdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3KTM, 5E4S
Resolution: 2.93→44.09 Å / SU ML: 0.3882 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 32.1231
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2828 1281 10.03 %
Rwork0.2399 11496 -
obs0.2441 12777 95.81 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 71.82 Å2
Refinement stepCycle: LAST / Resolution: 2.93→44.09 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2814 0 0 17 2831
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0032890
X-RAY DIFFRACTIONf_angle_d0.58663938
X-RAY DIFFRACTIONf_chiral_restr0.0498434
X-RAY DIFFRACTIONf_plane_restr0.0049510
X-RAY DIFFRACTIONf_dihedral_angle_d9.58641053
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.93-3.040.39751290.38491134X-RAY DIFFRACTION87.22
3.04-3.180.36331380.33751183X-RAY DIFFRACTION91.1
3.18-3.350.33291370.28121233X-RAY DIFFRACTION94.55
3.35-3.560.36871340.27241265X-RAY DIFFRACTION94.78
3.56-3.830.30841430.24221285X-RAY DIFFRACTION98.08
3.84-4.220.2641450.22651287X-RAY DIFFRACTION97.15
4.22-4.830.23431500.19361323X-RAY DIFFRACTION98.99
4.83-6.080.23261470.19671358X-RAY DIFFRACTION99.87
6.08-44.090.25751580.23161428X-RAY DIFFRACTION99.87
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
13.499800915572.89307939608-1.368332271973.58080357948-1.045905626871.71676489420.314141176183-0.1494001390560.2696580447680.622953694023-0.1858927752890.358134434087-0.220623824415-0.121601938456-0.1517150797590.4631594095350.004085776019130.1253227382130.284707106737-0.03592812979550.74089236834568.259547889155.512615368136.6891021392
20.6832273277070.805193231602-0.06001067180284.618783158820.7568037363132.096778838430.274577472338-0.3132574905240.1876775920030.103304103496-0.219185202280.0686638092936-0.2599394386180.0533574261147-0.01607928784490.253701088504-0.003913953862380.04980248401020.28794054254-0.001335567328890.71211436839595.945694343976.404466385919.8363886458
34.822971134720.329619606235-0.08548582881864.843508378733.110544473975.975675397070.0425216797905-0.6641887695990.01017935786680.1293136316660.2052081489040.084561986328-0.5731411746810.190714081272-0.2763646528830.533998371638-0.06950586249860.2411768451730.498522885483-0.06927695852420.9970794342108.190548084101.85675341539.9050852615
44.24163057021-0.518947254870.2569478056695.42415770159-1.21561126083.27412668907-0.281956475456-0.5214318284670.0041887895588-0.396934932684-0.365697326654-0.3187820627380.09187052280260.0279082660120.627069839510.3948986651510.1321778673170.2227834688090.3294185712150.05286043947081.3863006289696.623240376662.231734727915.0125777983
54.61057814673-0.9113346934782.389172462650.68745923241.185673580416.742991858010.252967460764-0.117384591143-1.736282311180.111271539780.0384769716538-0.07317782591451.9402233103-0.11492239967-0.2796718586180.6011205528090.05494386577580.002222830101290.3898869261580.06245655227911.57729013847100.38299621451.93902069216.4643534972
61.84068264614-1.227192710270.9560921593053.72579176222-1.086711975186.589296736820.0940079327460.206833081149-0.3017013966430.0643081883623-0.407585043208-0.682441256161.033012536211.085860887290.2736528601370.490667836990.281889032105-0.03594054330440.3596615721090.01080681732231.48315499874101.45803206755.603796149918.1127551549
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 598 through 737 )AA598 - 7371 - 140
22chain 'A' and (resid 738 through 812 )AA738 - 812141 - 215
33chain 'A' and (resid 813 through 901 )AA813 - 901216 - 298
44chain 'B' and (resid 124 through 141 )BB124 - 1411 - 18
55chain 'B' and (resid 142 through 173 )BB142 - 17319 - 50
66chain 'B' and (resid 174 through 192 )BB174 - 19251 - 68

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242):Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more