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Open data
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Basic information
Entry | Database: PDB / ID: 7mrs | ||||||
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Title | Zebrafish CNTN4 APPb complex | ||||||
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![]() | CELL ADHESION / Ig superfamily / amyloid precursor protein | ||||||
Function / homology | ![]() convergent extension / regulation of Notch signaling pathway / cell-cell adhesion mediator activity / vasculature development / axon extension / regulation of neuron differentiation / Golgi-associated vesicle / transition metal ion binding / axonogenesis / central nervous system development ...convergent extension / regulation of Notch signaling pathway / cell-cell adhesion mediator activity / vasculature development / axon extension / regulation of neuron differentiation / Golgi-associated vesicle / transition metal ion binding / axonogenesis / central nervous system development / axon guidance / brain development / cell-cell adhesion / neuron projection development / heparin binding / membrane => GO:0016020 / axon / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bouyain, S. / Karuppan, S.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Members of the vertebrate contactin and amyloid precursor protein families interact through a conserved interface. Authors: Karuppan, S.J. / Vogt, A. / Fischer, Z. / Ladutska, A. / Swiastyn, J. / McGraw, H.F. / Bouyain, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 180.9 KB | Display | ![]() |
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PDB format | ![]() | 125.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436 KB | Display | ![]() |
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Full document | ![]() | 438.9 KB | Display | |
Data in XML | ![]() | 14.6 KB | Display | |
Data in CIF | ![]() | 19.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7mqyC ![]() 7mrkC ![]() 7mrmC ![]() 7mrnC ![]() 7mroC ![]() 7mrqC ![]() 3ktmS ![]() 5e4sS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33217.207 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 21126.209 Da / Num. of mol.: 1 / Fragment: E1 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.96 % |
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Crystal grow | Temperature: 277 K / Method: batch mode / Details: 75 mM NaCl, 10 mM Na-HEPES pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Feb 10, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.93→89.77 Å / Num. obs: 13383 / % possible obs: 100 % / Redundancy: 10.4 % / Biso Wilson estimate: 58.35 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.165 / Rpim(I) all: 0.053 / Rrim(I) all: 0.174 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 2.93→3.1 Å / Redundancy: 10 % / Rmerge(I) obs: 1.163 / Mean I/σ(I) obs: 2 / Num. unique obs: 2111 / CC1/2: 0.697 / Rpim(I) all: 0.384 / Rrim(I) all: 1.227 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3KTM, 5E4S Resolution: 2.93→44.09 Å / SU ML: 0.3882 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 32.1231 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 71.82 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.93→44.09 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION
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