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- PDB-7lhk: High-Resolution Crystal Structure of a Lipin/Pah Phosphatidic Aci... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7lhk | ||||||||||||
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Title | High-Resolution Crystal Structure of a Lipin/Pah Phosphatidic Acid Phosphatase | ||||||||||||
![]() | Nuclear elongation and deformation protein | ||||||||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Domain/homology | ||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Khayyo, V.I. / Airola, M.V. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: High-resolution structure of phosphatidic acid phosphohydrolase from Tetrahymena thermophila Authors: Khayyo, V.I. / Airola, M.V. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 308 KB | Display | ![]() |
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PDB format | ![]() | 202.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6tzyS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 35758.023 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: SB210 / Gene: TTHERM_00215970 / Plasmid: ppSUMO Production host: ![]() ![]() ![]() References: UniProt: I7MFJ3 #2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.82 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Tt Pah2 in a ratio of 2:1 with well solution of 0.1M sodium cacodylate pH 6.5, 1 M NaCl, 10% glycerol, 30% PEG 600 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 23, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.95→55.25 Å / Num. obs: 720604 / % possible obs: 99.54 % / Redundancy: 12.6 % / Biso Wilson estimate: 30.57 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.08466 / Rpim(I) all: 0.02437 / Rrim(I) all: 0.08818 / Net I/σ(I): 16.77 |
Reflection shell | Resolution: 1.95→2.02 Å / Redundancy: 7.9 % / Rmerge(I) obs: 0.686 / Mean I/σ(I) obs: 2.87 / Num. unique obs: 5410 / CC1/2: 0.907 / CC star: 0.975 / Rpim(I) all: 0.2381 / Rrim(I) all: 0.686 / % possible all: 95.83 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 6TZY Resolution: 1.95→55.25 Å / SU ML: 0.1972 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 20.6774 / Stereochemistry target values: CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.45 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→55.25 Å
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Refine LS restraints |
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LS refinement shell |
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