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Open data
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Basic information
Entry | Database: PDB / ID: 1usk | ||||||
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Title | L-leucine-binding protein with leucine bound | ||||||
![]() | LEUCINE-SPECIFIC BINDING PROTEIN | ||||||
![]() | TRANSPORT PROTEIN / LEUCINE-BINDING PROTEIN / PROTEIN STRUCTURE / ABC TRANSPORT SYSTEMS | ||||||
Function / homology | ![]() branched-chain amino acid transport / L-leucine binding / phenylalanine transport / L-leucine transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Magnusson, U. / Salopek-Sondi, B. / Luck, L.A. / Mowbray, S.L. | ||||||
![]() | ![]() Title: X-Ray Structures of the Leucine-Binding Protein Illustrate Conformational Changes and the Basis of Ligand Specificity Authors: Magnusson, U. / Salopek-Sondi, B. / Luck, L.A. / Mowbray, S.L. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 259.2 KB | Display | ![]() |
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PDB format | ![]() | 210.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 410.2 KB | Display | ![]() |
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Full document | ![]() | 414.4 KB | Display | |
Data in XML | ![]() | 34.2 KB | Display | |
Data in CIF | ![]() | 54.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1usgC ![]() 1usiSC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37079.699 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-LEU / #3: Water | ChemComp-HOH / | Compound details | A COMPONENT OF THE LEUCINE-SPECIFIC TRANSPORT SYSTEM, WHICH IS ONE OF TWO PERIPLASMIC BINDING ...A COMPONENT OF THE LEUCINE-SPECIFIC TRANSPORT SYSTEM, WHICH IS ONE OF TWO PERIPLASMI | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.1 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5.6 / Details: PEG 4000, SODIUM CITRATE, PH 5.6, 2-PROPANOL | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Mar 15, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2→38 Å / Num. obs: 72985 / % possible obs: 79.1 % / Redundancy: 2.8 % / Rmerge(I) obs: 0.164 / Net I/σ(I): 4.6 |
Reflection shell | Resolution: 2→2.03 Å / Redundancy: 1.1 % / Rmerge(I) obs: 0.431 / Mean I/σ(I) obs: 1.4 / % possible all: 22.7 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 38 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.164 |
Reflection shell | *PLUS % possible obs: 22.7 % / Redundancy: 1.1 % / Rmerge(I) obs: 0.431 / Mean I/σ(I) obs: 1.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1USI Resolution: 2→72.55 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.907 / SU B: 7.129 / SU ML: 0.177 / Cross valid method: THROUGHOUT / ESU R: 0.295 / ESU R Free: 0.217 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.77 Å2
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Refinement step | Cycle: LAST / Resolution: 2→72.55 Å
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Refine LS restraints |
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