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- PDB-5npw: Structure of human ATG5-ATG16L1(ATG5BD) complex (C2) -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 5npw
TitleStructure of human ATG5-ATG16L1(ATG5BD) complex (C2)
Components
  • Autophagy protein 5
  • Autophagy-related protein 16-1
KeywordsCELL CYCLE / autophagy / ATG16L1 / ATG5
Function / homology
Function and homology information


otolith development / regulation of autophagosome maturation / positive regulation of viral translation / C-terminal protein lipidation / regulation of cytokine production involved in immune response / Atg8-family ligase activity / response to fluoride / Atg12-Atg5-Atg16 complex / antigen processing and presentation of endogenous antigen / negative regulation of dendrite extension ...otolith development / regulation of autophagosome maturation / positive regulation of viral translation / C-terminal protein lipidation / regulation of cytokine production involved in immune response / Atg8-family ligase activity / response to fluoride / Atg12-Atg5-Atg16 complex / antigen processing and presentation of endogenous antigen / negative regulation of dendrite extension / positive regulation of mucus secretion / vacuole-isolation membrane contact site / phagophore / negative regulation of autophagic cell death / positive regulation of stress granule assembly / ubiquitin-like protein transferase activity / cellular response to nitrosative stress / negative regulation of defense response to virus / microautophagy / ventricular cardiac muscle cell development / regulation of cilium assembly / aggrephagy / transferase complex / mucus secretion / mitochondria-associated endoplasmic reticulum membrane contact site / response to fungus / dendrite arborization / xenophagy / corpus callosum development / protein localization to phagophore assembly site / negative thymic T cell selection / phagophore assembly site membrane / piecemeal microautophagy of the nucleus / negative stranded viral RNA replication / regulation of release of sequestered calcium ion into cytosol / endolysosome membrane / cellular response to nitrogen starvation / negative regulation of cardiac muscle cell apoptotic process / negative regulation of phagocytosis / regulation of reactive oxygen species metabolic process / negative regulation of type I interferon production / axonal transport / Receptor Mediated Mitophagy / Macroautophagy / response to iron(II) ion / chaperone-mediated autophagy / heart contraction / autophagosome membrane / axoneme / blood vessel remodeling / autophagosome assembly / postsynaptic modulation of chemical synaptic transmission / regulation of postsynaptic membrane neurotransmitter receptor levels / mitophagy / protein-membrane adaptor activity / sperm midpiece / cardiac muscle cell apoptotic process / negative regulation of protein ubiquitination / positive regulation of autophagy / post-translational protein modification / autophagosome / negative regulation of innate immune response / PINK1-PRKN Mediated Mitophagy / hippocampus development / Negative regulators of DDX58/IFIH1 signaling / macroautophagy / autophagy / vasodilation / Schaffer collateral - CA1 synapse / phagocytic vesicle membrane / protein transport / chromatin organization / GTPase binding / defense response to virus / postsynapse / protein ubiquitination / response to xenobiotic stimulus / axon / innate immune response / glutamatergic synapse / protein-containing complex / identical protein binding / membrane / cytoplasm / cytosol
Similarity search - Function
Autophagy protein Apg5, helix rich domain / Ubiquitin-like (UB roll) - #620 / Autophagy-related protein 5 / Autophagy protein Atg5, helix rich domain / Autophagy protein Atg5, UblA domain / : / : / : / Autophagy protein ATG5, UblB domain / Autophagy protein ATG5, alpha-helical bundle region ...Autophagy protein Apg5, helix rich domain / Ubiquitin-like (UB roll) - #620 / Autophagy-related protein 5 / Autophagy protein Atg5, helix rich domain / Autophagy protein Atg5, UblA domain / : / : / : / Autophagy protein ATG5, UblB domain / Autophagy protein ATG5, alpha-helical bundle region / Autophagy protein ATG5, UblA domain / Autophagy-related protein 16 / Autophagy-related protein 16 domain / Autophagy protein 16 (ATG16) / Serum Albumin; Chain A, Domain 1 / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / WD domain, G-beta repeat / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Roll / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Autophagy-related protein 16-1 / Autophagy protein 5
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.1 Å
AuthorsArchna, A. / Scrima, A.
CitationJournal: Acta Crystallogr F Struct Biol Commun / Year: 2017
Title: Identification, biochemical characterization and crystallization of the central region of human ATG16L1.
Authors: Archna, A. / Scrima, A.
History
DepositionApr 19, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 11, 2017Provider: repository / Type: Initial release
Revision 1.1Nov 1, 2017Group: Database references / Category: citation
Item: _citation.journal_abbrev / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Autophagy protein 5
B: Autophagy-related protein 16-1
C: Autophagy protein 5
D: Autophagy-related protein 16-1
E: Autophagy protein 5
F: Autophagy-related protein 16-1
G: Autophagy protein 5
H: Autophagy-related protein 16-1


Theoretical massNumber of molelcules
Total (without water)271,9118
Polymers271,9118
Non-polymers00
Water1267
1
A: Autophagy protein 5
B: Autophagy-related protein 16-1


Theoretical massNumber of molelcules
Total (without water)67,9782
Polymers67,9782
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2210 Å2
ΔGint-18 kcal/mol
Surface area14910 Å2
MethodPISA
2
C: Autophagy protein 5
D: Autophagy-related protein 16-1


Theoretical massNumber of molelcules
Total (without water)67,9782
Polymers67,9782
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2470 Å2
ΔGint-13 kcal/mol
Surface area14770 Å2
MethodPISA
3
E: Autophagy protein 5
F: Autophagy-related protein 16-1


Theoretical massNumber of molelcules
Total (without water)67,9782
Polymers67,9782
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2180 Å2
ΔGint-18 kcal/mol
Surface area14640 Å2
MethodPISA
4
G: Autophagy protein 5
H: Autophagy-related protein 16-1


Theoretical massNumber of molelcules
Total (without water)67,9782
Polymers67,9782
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2410 Å2
ΔGint-14 kcal/mol
Surface area14850 Å2
MethodPISA
Unit cell
Length a, b, c (Å)200.860, 75.990, 142.440
Angle α, β, γ (deg.)90.000, 131.410, 90.000
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resseq 4 or (resid 5 and (name...
21(chain C and ((resid 4 and (name N or name...
31(chain E and ((resid 4 and (name N or name...
41(chain G and ((resid 4 and (name N or name...
12(chain D and ((resid 10 and (name N or name...
22(chain B and ((resid 10 and (name N or name...
32(chain F and (resseq 10:14 or (resid 15 and (name...
42(chain H and ((resid 10 and (name N or name...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA411
121LYSLYSLYSLYS(chain A and (resseq 4 or (resid 5 and (name...AA512
131ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA4 - 27511 - 282
141ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA4 - 27511 - 282
151ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA4 - 27511 - 282
161ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA4 - 27511 - 282
171ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA4 - 27511 - 282
181ASPASPASPASP(chain A and (resseq 4 or (resid 5 and (name...AA4 - 27511 - 282
211ASPASPASPASP(chain C and ((resid 4 and (name N or name...CC411
221METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
231METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
241METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
251METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
261METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
271METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
281METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
291METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
2101METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
2111METMETTHRTHR(chain C and ((resid 4 and (name N or name...CC1 - 2748 - 281
311ASPASPASPASP(chain E and ((resid 4 and (name N or name...EE411
321ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
331ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
341ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
351ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
361ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
371ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
381ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
391ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
3101ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
3111ASPASPTHRTHR(chain E and ((resid 4 and (name N or name...EE3 - 27410 - 281
411ASPASPASPASP(chain G and ((resid 4 and (name N or name...GG411
421METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
431METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
441METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
451METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
461METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
471METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
481METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
491METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
4101METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
4111METMETASPASP(chain G and ((resid 4 and (name N or name...GG1 - 2758 - 282
112ARGARGARGARG(chain D and ((resid 10 and (name N or name...DD104
122GLYGLYASPASP(chain D and ((resid 10 and (name N or name...DD8 - 472 - 41
132GLYGLYASPASP(chain D and ((resid 10 and (name N or name...DD8 - 472 - 41
142GLYGLYASPASP(chain D and ((resid 10 and (name N or name...DD8 - 472 - 41
152GLYGLYASPASP(chain D and ((resid 10 and (name N or name...DD8 - 472 - 41
162GLYGLYASPASP(chain D and ((resid 10 and (name N or name...DD8 - 472 - 41
212ARGARGARGARG(chain B and ((resid 10 and (name N or name...BB104
222GLYGLYLEULEU(chain B and ((resid 10 and (name N or name...BB9 - 483 - 42
232GLYGLYLEULEU(chain B and ((resid 10 and (name N or name...BB9 - 483 - 42
242GLYGLYLEULEU(chain B and ((resid 10 and (name N or name...BB9 - 483 - 42
252GLYGLYLEULEU(chain B and ((resid 10 and (name N or name...BB9 - 483 - 42
262GLYGLYLEULEU(chain B and ((resid 10 and (name N or name...BB9 - 483 - 42
312ARGARGLYSLYS(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 144 - 8
322ARGARGARGARG(chain F and (resseq 10:14 or (resid 15 and (name...FF159
332ARGARGLEULEU(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 484 - 42
342ARGARGLEULEU(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 484 - 42
352ARGARGLEULEU(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 484 - 42
362ARGARGLEULEU(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 484 - 42
372ARGARGLEULEU(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 484 - 42
382ARGARGLEULEU(chain F and (resseq 10:14 or (resid 15 and (name...FF10 - 484 - 42
412ARGARGARGARG(chain H and ((resid 10 and (name N or name...HH104
422GLYGLYLEULEU(chain H and ((resid 10 and (name N or name...HH8 - 482 - 42
432GLYGLYLEULEU(chain H and ((resid 10 and (name N or name...HH8 - 482 - 42
442GLYGLYLEULEU(chain H and ((resid 10 and (name N or name...HH8 - 482 - 42
452GLYGLYLEULEU(chain H and ((resid 10 and (name N or name...HH8 - 482 - 42
462GLYGLYLEULEU(chain H and ((resid 10 and (name N or name...HH8 - 482 - 42

NCS ensembles :
ID
1
2

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Components

#1: Protein
Autophagy protein 5 / APG5-like / Apoptosis-specific protein


Mass: 33187.992 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ATG5, APG5L, ASP / Plasmid: pET15b-derived / Production host: Escherichia coli (E. coli) / References: UniProt: Q9H1Y0
#2: Protein
Autophagy-related protein 16-1 / APG16-like 1


Mass: 34789.879 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ATG16L1, APG16L, UNQ9393/PRO34307 / Plasmid: pCOLA-derived / Production host: Escherichia coli (E. coli) / References: UniProt: Q676U5
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.4 M KCl and 18 % PEG 3350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Sep 8, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.1→48.316 Å / Num. obs: 29428 / % possible obs: 99.1 % / Observed criterion σ(I): -3 / Redundancy: 3.698 % / Biso Wilson estimate: 62.2 Å2 / CC1/2: 0.992 / Rmerge(I) obs: 0.126 / Rrim(I) all: 0.147 / Χ2: 0.962 / Net I/σ(I): 9.48
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rrim(I) all% possible all
3.1-3.23.8410.7452.0826430.6870.86599.3
3.2-3.33.7980.5043.0423660.8260.58699.3
3.3-43.5760.2285.9106140.9560.26899.2
4-63.8260.09412.9896460.990.10999.1
6-103.5050.0616.3232300.9960.0798.9
10-48.3163.7710.03727.669290.9970.04396.7

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
XSCALEdata scaling
PHASERphasing
PHENIX1.10.1_2155refinement
PDB_EXTRACT3.22data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4GDK

4gdk


Resolution: 3.1→48.316 Å / SU ML: 0.5 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 28.52
RfactorNum. reflection% reflection
Rfree0.2639 1472 5 %
Rwork0.2175 --
obs0.2198 29416 99.6 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 206.4 Å2 / Biso mean: 68.1332 Å2 / Biso min: 4.46 Å2
Refinement stepCycle: final / Resolution: 3.1→48.316 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9945 0 0 7 9952
Biso mean---18.24 -
Num. residues----1221
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00310225
X-RAY DIFFRACTIONf_angle_d0.50413895
X-RAY DIFFRACTIONf_chiral_restr0.0391499
X-RAY DIFFRACTIONf_plane_restr0.0031783
X-RAY DIFFRACTIONf_dihedral_angle_d8.9636104
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A5734X-RAY DIFFRACTION5.965TORSIONAL
12C5734X-RAY DIFFRACTION5.965TORSIONAL
13E5734X-RAY DIFFRACTION5.965TORSIONAL
14G5734X-RAY DIFFRACTION5.965TORSIONAL
21D812X-RAY DIFFRACTION5.965TORSIONAL
22B812X-RAY DIFFRACTION5.965TORSIONAL
23F812X-RAY DIFFRACTION5.965TORSIONAL
24H812X-RAY DIFFRACTION5.965TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 11 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
3.1001-3.20020.37091330.357525132646
3.2002-3.31450.34311330.294425352668
3.3145-3.44720.33451330.286925332666
3.4472-3.6040.29821320.254825022634
3.604-3.7940.28491340.223725372671
3.794-4.03160.26251320.207525162648
4.0316-4.34270.24171340.190125392673
4.3427-4.77940.20441330.165125302663
4.7794-5.47010.23621340.182925552689
5.4701-6.88860.26771350.229225522687
6.8886-48.32190.24261390.197426322771
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.92740.12670.86731.72770.72664.7994-0.0657-0.01270.1704-0.11820.1835-0.1911-0.4669-0.021-0.12130.37770.0630.03950.4420.03410.4205-61.8908-5.8694118.2184
23.9635-1.48271.17952.8216-0.96814.8322-0.07870.108-0.2933-0.18860.15350.05240.28290.025-0.05840.48640.0060.10110.4712-0.02030.3993-61.8931-26.26110.7073
32.6275-0.09190.00097.52763.60325.2194-0.5016-0.2914-0.08770.46830.12170.79830.3745-0.460.4280.3982-0.06690.0060.57020.03010.5086-71.6716-16.9182129.4802
41.57490.0753-0.3912.1651.93016.88650.1085-0.09640.14340.0384-0.1834-0.0685-0.05230.14680.03160.3669-0.02830.0210.43080.02570.4242-48.9649-16.161588.1377
52.6259-0.80030.25383.7756-0.19652.04830.0188-0.15080.0443-0.07610.0011-0.14770.02620.1701-0.0070.3768-0.0057-0.04110.3707-0.03560.3651-41.9863-31.616872.5577
61.5104-2.2164-2.74325.5825.08175.3982-0.14410.05290.0074-0.15410.06160.4332-0.1439-0.12580.06190.5358-0.0086-0.14710.55110.09120.4721-59.4183-17.010173.2412
73.5685-1.302-0.48210.6140.762.61530.0827-0.2048-0.58220.0111-0.1297-0.230.61750.57570.37220.5987-0.0843-0.10760.3720.15680.3394-80.7059-17.522385.4974
84.42021.95211.13965.8574-1.2023.2531-0.00970.43310.63570.11730.1380.4541-0.3254-0.0546-0.10630.4331-0.04570.06430.47840.09060.4616-80.019-2.228574.339
93.69950.14860.22823.79362.29294.84360.0285-0.2036-0.23870.1066-0.30730.27860.0848-0.29030.27280.4387-0.00690.04640.48770.01480.5099-94.9717-23.132578.8527
106.06624.9134-0.30054.0993-0.30923.43330.40570.2546-2.01051.28250.0302-1.12010.42680.2841-0.40360.72960.1277-0.0460.5362-0.01330.8826-77.8185-30.397183.3097
113.8412-2.2462-0.43632.43671.20016.7899-0.5345-0.6060.16471.01091.1215-0.5248-0.1279-0.1391-0.53660.41930.08190.06940.5938-0.00570.4705-67.6853-5.47888.561
128.4896-2.11582.36013.1897-0.40472.84730.1138-0.49890.45060.2014-0.20.1486-0.1433-0.55820.11220.5104-0.0375-0.00830.5188-0.00110.4143-75.447-12.4204152.5049
1330.4228-0.51013.1809-0.34423.34230.15-0.1357-0.1490.2109-0.2443-0.32770.12160.11940.07570.3701-0.0551-0.03290.37140.10110.4743-60.1964-25.8383162.2421
143.4961-1.50732.91984.8782-1.23366.04810.41970.4979-0.6977-0.61890.1835-0.92060.11330.1629-0.47980.50960.02380.09870.34520.08610.8098-52.2309-23.571145.4839
156.1462-0.46882.18172.4466-2.77966.26870.2325-0.88110.21890.3324-0.23210.176-0.68651.14190.07140.46990.00980.00570.5815-0.02570.5161-68.9196-0.5406140.8652
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 136 )A4 - 136
2X-RAY DIFFRACTION2chain 'A' and (resid 137 through 275 )A137 - 275
3X-RAY DIFFRACTION3chain 'B' and (resid 9 through 48 )B9 - 48
4X-RAY DIFFRACTION4chain 'C' and (resid 1 through 117 )C1 - 117
5X-RAY DIFFRACTION5chain 'C' and (resid 118 through 274 )C118 - 274
6X-RAY DIFFRACTION6chain 'D' and (resid 8 through 47 )D8 - 47
7X-RAY DIFFRACTION7chain 'E' and (resid 3 through 22 )E3 - 22
8X-RAY DIFFRACTION8chain 'E' and (resid 23 through 117 )E23 - 117
9X-RAY DIFFRACTION9chain 'E' and (resid 118 through 274 )E118 - 274
10X-RAY DIFFRACTION10chain 'F' and (resid 10 through 28 )F10 - 28
11X-RAY DIFFRACTION11chain 'F' and (resid 29 through 48 )F29 - 48
12X-RAY DIFFRACTION12chain 'G' and (resid 1 through 117 )G1 - 117
13X-RAY DIFFRACTION13chain 'G' and (resid 118 through 275 )G118 - 275
14X-RAY DIFFRACTION14chain 'H' and (resid 8 through 28 )H8 - 28
15X-RAY DIFFRACTION15chain 'H' and (resid 29 through 48 )H29 - 48

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