+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 7enc | ||||||
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タイトル | TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED) | ||||||
要素 |
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キーワード | TRANSCRIPTION / mediator / preinitiation complex / transcription initiation | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / spermine transport / negative regulation of MHC class I biosynthetic process / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / MMXD complex / core TFIIH complex portion of holo TFIIH complex / SAGA complex assembly ...positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / spermine transport / negative regulation of MHC class I biosynthetic process / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / MMXD complex / core TFIIH complex portion of holo TFIIH complex / SAGA complex assembly / lateral mesodermal cell differentiation / androgen biosynthetic process / positive regulation of G0 to G1 transition / DNA-templated transcription open complex formation / allantois development / mammary gland branching involved in thelarche / retinal pigment epithelium development / pre-snoRNP complex / Cytosolic iron-sulfur cluster assembly / nucleotide-excision repair, DNA duplex unwinding / TFIIH-class transcription factor complex binding / G0 to G1 transition / thyroid hormone receptor signaling pathway / negative regulation of protein autoubiquitination / central nervous system myelin formation / positive regulation of core promoter binding / transcription factor TFTC complex / RNA polymerase II core complex assembly / core mediator complex / positive regulation of mitotic recombination / meiotic sister chromatid cohesion / negative regulation of MHC class II biosynthetic process / hair follicle maturation / RNA polymerase transcription factor SL1 complex / ventricular system development / snRNA transcription by RNA polymerase II / hair cell differentiation / regulation of cell cycle G1/S phase transition / RNA polymerase I general transcription initiation factor activity / regulation of vitamin D receptor signaling pathway / nucleotide-excision repair factor 3 complex / transcription factor TFIIE complex / phosphatase activator activity / nucleotide-excision repair, preincision complex assembly / SLIK (SAGA-like) complex / CAK-ERCC2 complex / RNA polymerase III general transcription initiation factor activity / UV protection / transcription factor TFIIK complex / embryonic cleavage / transcription open complex formation at RNA polymerase II promoter / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / RNA polymerase I core promoter sequence-specific DNA binding / TFIIF-class transcription factor complex binding / hepatocyte differentiation / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / transcriptional start site selection at RNA polymerase II promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / ventricular trabecula myocardium morphogenesis / positive regulation of response to cytokine stimulus / thyroid hormone generation / transcription factor TFIIF complex / DNA 5'-3' helicase / mediator complex / nuclear retinoic acid receptor binding / maintenance of protein location in nucleus / RNA Polymerase III Abortive And Retractive Initiation / adult heart development / positive regulation of androgen receptor activity / positive regulation of keratinocyte differentiation / Generic Transcription Pathway / transcription factor TFIIA complex 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Sus scrofa (ブタ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.13 Å | ||||||
データ登録者 | Chen, X. / Qi, Y. / Wang, X. / Wu, Z. / Yin, X. / Li, J. / Liu, W. / Xu, Y. | ||||||
引用 | ジャーナル: Science / 年: 2021 タイトル: Structures of the human Mediator and Mediator-bound preinitiation complex. 著者: Xizi Chen / Xiaotong Yin / Jiabei Li / Zihan Wu / Yilun Qi / Xinxin Wang / Weida Liu / Yanhui Xu / 要旨: The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26- ...The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26-subunit Mediator stimulates transcription and cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the Pol II C-terminal domain (CTD). We determined the structures of human Mediator in the Tail module-extended (at near-atomic resolution) and Tail-bent conformations and structures of TFIID-based PIC-Mediator (76 polypeptides, ~4.1 megadaltons) in four distinct conformations. PIC-Mediator assembly induces concerted reorganization (Head-tilting and Middle-down) of Mediator and creates a Head-Middle sandwich, which stabilizes two CTD segments and brings CTD to CDK7 for phosphorylation; this suggests a CTD-gating mechanism favorable for phosphorylation. The TFIID-based PIC architecture modulates Mediator organization and TFIIH stabilization, underscoring the importance of TFIID in orchestrating PIC-Mediator assembly. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7enc.cif.gz | 4 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7enc.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 7enc.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7enc_validation.pdf.gz | 1.5 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7enc_full_validation.pdf.gz | 1.7 MB | 表示 | |
XML形式データ | 7enc_validation.xml.gz | 420.4 KB | 表示 | |
CIF形式データ | 7enc_validation.cif.gz | 709.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/en/7enc ftp://data.pdbj.org/pub/pdb/validation_reports/en/7enc | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
+Mediator of RNA polymerase II transcription subunit ... , 23種, 23分子 amdfgrtjknqsuvzcebloiwx
+Isoform 2 of Mediator of RNA polymerase II transcription subunit ... , 2種, 2分子 hp
+タンパク質 , 10種, 10分子 089EADPDQPAPJPKPL
+General transcription factor IIH subunit ... , 5種, 5分子 12345
+General transcription and DNA repair factor IIH helicase subunit ... , 2種, 2分子 67
+Transcription initiation factor ... , 16種, 22分子 EBDADBDDDdDEDeDFDfDGDHDIDiDJDjDLDlDcDkDmDOBA
+General transcription factor IIF subunit ... , 2種, 2分子 FBFA
+DNA鎖 , 2種, 2分子 XY
+DNA-directed RNA ... , 8種, 8分子 PBPCPDPEPFPGPHPI
+非ポリマー , 3種, 18分子
+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED) タイプ: COMPLEX / Entity ID: #1-#70 / 由来: MULTIPLE SOURCES |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
由来(組換発現) | 生物種: Homo sapiens (ヒト) |
緩衝液 | pH: 7.9 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 50 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
-解析
CTF補正 | タイプ: NONE |
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3次元再構成 | 解像度: 4.13 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 67732 / 対称性のタイプ: POINT |