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Basic information
Entry | Database: PDB / ID: 7efo | ||||||||||||
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Title | LptB2FG-LPS from Klebsiella pneumoniae in nanodiscs | ||||||||||||
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![]() | MEMBRANE PROTEIN / ABC transporter | ||||||||||||
Function / homology | ![]() ATPase-coupled transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex / transmembrane transport / hydrolase activity / ATP binding / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.85 Å | ||||||||||||
![]() | Luo, Q. / Shi, H. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structures of LptBFG and LptBFGC from Klebsiella pneumoniae in complex with lipopolysaccharide. Authors: Qingshan Luo / Huigang Shi / Xueqing Xu / ![]() Abstract: Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven ...Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven lipopolysaccharide transport proteins (LptA-G). LptBFG, an type VI ATP-binding cassette (ABC) transporter, forms a stable complex with LptC, extracts LPS from the IM and powers LPS transport to the OM. Here we report the cryo-EM structures of LptBFG and LptBFGC from Klebsiella pneumoniae in complex with LPS. The KpLptBFG-LPS structure provides detailed interactions between LPS and the transporter, while the KpLptBFGC-LPS structure may represent an intermediate state that the transmembrane helix of LptC has not been fully inserted into the transmembrane domains of LptBFG. | ||||||||||||
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-Related structure data
Related structure data | ![]() 31088MC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Assembly
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Components
#1: Protein | Mass: 26879.768 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: lptB_3, lptB / Production host: ![]() ![]() #2: Protein | | Mass: 40431.551 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: lptF / Production host: ![]() ![]() #3: Protein | | Mass: 39662.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: lptG / Production host: ![]() ![]() #4: Chemical | ChemComp-JSG / ( | |
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